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91.
BACKGROUND AND AIMS: Physiological and architectural plant models have originally been developed for different purposes and therefore have little in common, thus making combined applications difficult. There is, however, an increasing demand for crop models that simulate the genetic and resource-dependent variability of plant geometry and architecture, because man is increasingly able to transform plant production systems through combined genetic and environmental engineering. MODEL: GREENLAB is presented, a mathematical plant model that simulates interactions between plant structure and function. Dual-scale automaton is used to simulate plant organogenesis from germination to maturity on the basis of organogenetic growth cycles that have constant thermal time. Plant fresh biomass production is computed from transpiration, assuming transpiration efficiency to be constant and atmospheric demand to be the driving force, under non-limiting water supply. The fresh biomass is then distributed among expanding organs according to their relative demand. Demand for organ growth is estimated from allometric relationships (e.g. leaf surface to weight ratios) and kinetics of potential growth rate for each organ type. These are obtained through parameter optimization against empirical, morphological data sets by running the model in inverted mode. Potential growth rates are then used as estimates of relative sink strength in the model. These and other 'hidden' plant parameters are calibrated using the non-linear, least-square method. KEY RESULTS AND CONCLUSIONS: The model reproduced accurately the dynamics of plant growth, architecture and geometry of various annual and woody plants, enabling 3D visualization. It was also able to simulate the variability of leaf size on the plant and compensatory growth following pruning, as a result of internal competition for resources. The potential of the model's underlying concepts to predict the plant's phenotypic plasticity is discussed. 相似文献
92.
玉米及马齿苋叶片SOD活性诱导研究 总被引:1,自引:0,他引:1
以玉米幼苗及马齿苋作材料,通过甘露醇、H2O2、臭氧和强光等胁迫后,用NBT光化还原抑制法测定叶中SOD活性的变化。臭氧和强光能诱导玉米叶片SOD活性增加。0.5mol/L甘露醇处理玉米叶片12h,SOD活性上升,至48h后下降;在该甘露醇溶液中另外加入10^-2mol/L H2O2;处理12h后SOD活性基本不变。对玉米叶片单独用10^-2mol/L H2O2诱导,30min内SOD活性上升到最高值,随着处理时间的延长又逐渐下降。用耐强光、耐干旱的野生马齿苋作为材料,与玉米相比,其叶片SOD基础活性低于后者;若予以正午强光结合渗透胁迫2h,其叶中SOD活性增幅超过玉米,从种间比较的角度旁证了SOD在抗逆性中的作用。推测植物中存在比活性氧更为直接的物理诱导机制。 相似文献
93.
<正>Reactive oxygen species arise(ROS)in the mitochondria as byproducts of respiration and oxidase activity and have important roles in many physiological and pathophysiological conditions.The current literature indicate that excessive levels of ROS can cause oxidative stress and that lots of evidences link ROS and oxidative stress to the pathogenesis of type 2 diabetes mellitus(T2DM)and development of complications.Several studies have shown elevated extraand intracellular glucose concentrations result in oxidative stress both in animal models of diabetes and in diabetic patients[1].And ROS can contribute to the development and progression of diabetes and related complications by directly damaging DNA,proteins,and lipids or indirectly activating a number of cellular stress-sensitive pathways to induce damage to tissues such as isletβcells[2]. 相似文献
94.
考察5种萃取体系(A:正己烷,B:正己烷/乙醇,C:正己烷/异丙醇,D:氯仿/甲醇,E:氯仿/乙醇)对小球藻(Chlorella phyrenoidosa)油脂的提取效果及藻渣成分的影响。实验结果表明:不同的萃取体系下,油脂得率为D(12.27%)、E(8.87%)、C(7.71%)、B(6.80%)、A(3.91%),藻渣蛋白含量为A(52.60%)、E(46.23%)、B(40.19%)、C(39.52%)、D(32.52%),藻渣碳水化合物含量为A(23.28%)、E(16.15%)、B(13.24%)、D(13.50%)、C(9.06%);藻渣色素含量为A(1.75%)、E(1.29%)、B(1.14%)、C(0.96%)、D(0.58%);藻渣灰分含量为D(3.63%)、E(2.94%)、C(2.23%)、B(2.25%)、A(1.48%)。综合考虑微藻生物柴油的生产及藻渣的可利用性,V(氯仿)/V(乙醇)=1是一种油脂萃取效果较好,藻渣营养成分损失较小的小球藻油脂萃取体系。 相似文献
95.
96.
从中国发病鸡中分离的鸡减蛋综合征病毒(EggDropSyndromVirus,EDSV)弱毒株(AA-2),其基因组全长约为33kb。用限制性内切酶HindⅢ水解EDSV全基因组,构建了以pBluescriptⅡ(KS+)为载体的右末端片段的克隆(约4.2kb),对其进行了序列测定和结构分析。该片段全长4183个碱基对(bp),位于基因组右末端87.3m.u.-100m.u.。结果显示,该片段与哺乳动物腺病毒右末端E4区结构不同,与禽Ⅰ型腺病毒代表株CELO右末端片段亦无同源性。本文为深入了解EDSV基因组结构特点,EDSV与其他腺病毒基因结构与功能的进化关系和EDSV载体的构建奠定了分子生物学基础 相似文献
97.
【目的】研究pH信号通路(Pal)在重寄生真菌盾壳霉与寄主核盘菌互作过程中的作用。【方法】从盾壳霉全基因组信息中分析获得了6个Pal相关基因CmpalA、CmpalB、CmpalC、CmpalF、CmpalH和CmpalI的全编码序列和氨基酸序列,通过PEG介导的原生质转化技术获得了CmpalA、CmpalB、CmpalC、CmpalF和CmpalH等5个基因的敲除突变体,分析这些敲除突变体与野生型在菌落培养性状、重寄生能力、降解草酸能力、产生抗真菌物质能力等方面的差异。【结果】与野生型相比,在pH 6–8的条件下,5个Pal相关基因敲除突变体的菌丝生长受到显著抑制,这说明缺失Pal相关基因使盾壳霉对高pH值环境更加敏感。菌核重寄生试验发现5个Pal相关基因敲除突变体的重寄生能力均显著低于野生型。qRT-PCR试验结果表明,敲除Pal相关基因之后导致重寄生相关酶基因Cmch1、Cmg1和Cmsp1的表达量显著降低,而且pH信号通路下游的CmpacC基因的表达量也显著降低。Pal相关基因敲除突变体在pH 6条件下对草酸盐的降解能力显著高于野生型,同时这5个突变体在pH 8条件下产生抗真菌物质能力也显著高于野生型。【结论】pH信号通路相关基因的缺失影响盾壳霉对环境pH的响应。pH信号通路在盾壳霉与核盘菌互作中发挥重要作用,不仅影响盾壳霉的重寄生作用,而且还影响盾壳霉的草酸降解作用和抗真菌作用。 相似文献
98.
Primulina petrocosmeoides Bo Pan & Fang Wen sp. nov. (Gesneriaceae) from Guangxi, China, is described and illustrated. This new species is similar to P. weii Mich. Möller & A. Weber, but differs from it in leaf blade ovate to elliptical, 1.0 × 0.8 to 2.5 × 2.0 cm, leaf base broadly cuneate, cymes 5–16, 2–6‐flowered, bracts narrowly lanceolate, calyx lobes lanceolate, 4.0–6.5 mm long, corolla bluish purple, 1.2–1.5 cm long, pubescent outside but glabrous inside, filaments purple, pubescent, staminodes 3, stigma trapezoid with its apex lobed to the middle and with dense short papillae. 相似文献
99.
Insulation and wiring specificity of BceR‐like response regulators and their target promoters in Bacillus subtilis 下载免费PDF全文
Chong Fang Anna Nagy‐Staroń Martin Grafe Ralf Heermann Kirsten Jung Susanne Gebhard Thorsten Mascher 《Molecular microbiology》2017,104(1):16-31
BceRS and PsdRS are paralogous two‐component systems in Bacillus subtilis controlling the response to antimicrobial peptides. In the presence of extracellular bacitracin and nisin, respectively, the two response regulators (RRs) bind their target promoters, PbceA or PpsdA, resulting in a strong up‐regulation of target gene expression and ultimately antibiotic resistance. Despite high sequence similarity between the RRs BceR and PsdR and their known binding sites, no cross‐regulation has been observed between them. We therefore investigated the specificity determinants of PbceA and PpsdA that ensure the insulation of these two paralogous pathways at the RR–promoter interface. In vivo and in vitro analyses demonstrate that the regulatory regions within these two promoters contain three important elements: in addition to the known (main) binding site, we identified a linker region and a secondary binding site that are crucial for functionality. Initial binding to the high‐affinity, low‐specificity main binding site is a prerequisite for the subsequent highly specific binding of a second RR dimer to the low‐affinity secondary binding site. In addition to this hierarchical cooperative binding, discrimination requires a competition of the two RRs for their respective binding site mediated by only slight differences in binding affinities. 相似文献
100.
Gao Y Yue W Zhang P Li L Xie X Yuan H Chen L Liu D Yan F Pei X 《Biochemical and biophysical research communications》2005,335(2):343-350
spindlin1, a novel human gene recently isolated by our laboratory, is highly homologous to mouse spindlin gene. In this study, we cloned cDNA full-length of this novel gene and send it to GenBank database as spindlin1 (Homo sapiens spindlin1) with Accession No. AF317228. In order to investigate the function of spindlin1, we studied further the subcellular localization of Spindlin1 protein and the effects of spindlin1 overexpression in NIH3T3 cells. The results showed that the fusion protein pEGFP-N1-spindlin1 was located in the nucleus and the C-terminal is correlated with nuclear localization of Spindlin1 protein. NIH3T3 cells which could stably express spindlin1 as a result of RT-PCR analysis compared with the control cells displayed a complete morphological change; made cell growth faster; and increased the percentage of cells in G2/M and S phase. Furthermore, overexpressed spindlin1 cells formed colonies in soft agar in vitro and formed tumors in nude mice. Our findings provide direct evidence that spindlin1 gene may contribute to tumorigenesis. 相似文献