一例智力低下患者7q~ 标记染色体的来源鉴定

以人类染色体显微切割、PCR技术构建的现有人类染色体特异性和染色体区带特异性探针池作为绘画探针,采用正向染色体绘画技术,结合染色体筛查方法,查明了一例7q~ 标记染色体患者的染色体附加片段来源于3q26→3qter。确定该患者的核型为46,XX,-7, der(7)t(7;3)(7pter→7q32::3q26→3qter)。应用这个策略,能够快速有效地鉴定标记染色体的来源。     

Triton X－100对紫膜蛋白的效应

本文采用同步辐射小角Ｘ射线散射方法研究了用非离子表面活性剂ＴｒｉｔｏｎＸ—１００处理后的嗜盐菌紫膜及其视紫红质蛋白结构的变化。实验结果表明,用不同浓度的ＴｒｉｔｏｎＸ—１００处理紫膜碎片时,紫膜及其蛋白所处的状态有着很大变化。     

壳聚糖固定化纤维素酶的研究

以蟹壳为原料提取壳聚糖,用戊二醛作交联剂,将纤维素酶固定于壳聚糖上．同时探讨了一定量干壳聚糖载体与交联剂浓度、给酶量等关系的最适固定化酶条件,并对固定化酶的热稳定性、操作稳定性、米氏常数、最适温度、离子强度的影响及使用半衰期等理化性质进行了探讨．     

小麦形成大穗的生态学基础

研究了不同光、温条件下３个小麦品种的生育特征、幼穗分化、幼穗生长和穗部性状,探讨了促进穗大粒多、提高小麦单产的可行途径．结果表明,因播种期的不同而导致的小麦生长期间光、温条件的不同,使之各生育期出现的时间及其持续的天数有了较大的差异．小麦幼穗分化的历期天数多与平均气温和平均日照长度呈显著或极显著负相关．不同的积温是幼穗生长量和每穗总小穗数产生差异的重要原因．在条件适宜的麦区,可望通过引种适宜品种并适当提早播种期,以促进德大粒多、实现单产的明显提高．     

献血员抗－HCV检测报告

对１５８７名献血员进行了抗－ＨＣＶ检测,总阳性率５．６０％,在不同职业中农民较高,占６．７２％,３０岁以上为１２．７６％。我站自改进采浆工艺,严格使用一次性消毒器具后,未发现ＨＣＶ交叉感染。职业献血者抗－ＨＣＶ阳性率高达３５．４８％,易造成输血后ＨＣＶ感染。对ＨＣＶ感染ＯＤ值阳性３０人又进行４－６个月的观察复测,其中１６人ＯＤ值下降,６人上升,认为ＯＤ值的变化与自限性ＨＣＶ感染有关。     

A NEW SPECIES OF GENUS GRYPOCENTRUS RUTHE (HYMENOPTERA: ICHNEUMONIDAE)

Abstract  One new species of Grypocentrus , namely G. kasparyani , collected in Shenyang, China, is described.     

发情期小灵猫行为的研究

本文对笼养小灵猫发情期的繁殖行为进行了观察。结果表明：小灵猫一年有两次发情期,绝大部分的小灵猫在春季（２－４月）发情,少数个体在秋季（８月底至９月）出现第２次发情。发情期雄性小灵猫频繁地发出求偶叫声和增加擦香次数。雄猫的擦香行为和求偶叫声可促使雌猫的发情,发情期一系列性行为对雌雄交配的成功与否有重要的意义。     

The DnaJ domain of polyomavirus large T antigen is required to regulate Rb family tumor suppressor function.

Tumor suppressors of the retinoblastoma susceptibility gene family regulate cell growth and differentiation. Polyomavirus large T antigens (large T) bind Rb family members and block their function. Mutations of large T sequences conserved with the DnaJ family affect large T binding to a cellular DnaK, heat shock protein 70. The same mutations abolish large T activation of E2F-containing promoters and Rb binding-dependent large T activation of cell cycle progression. Cotransfection of a cellular DnaJ domain blocks wild-type large T action, showing that the connection between the chaperone system and tumor suppressors is direct. Although they are inactive in assays dependent on Rb family binding, mutants in the J region retain the ability to associate with pRb, p107, and p130. This suggests that binding of Rb family members by large T is not sufficient for their inactivation and that a functional J domain is required as well. This work connects the DnaJ and DnaK molecular chaperones to regulation of tumor suppressors by polyomavirus large T.     

Effects of acute hyperoxic exposure on solute fluxes across the blood-gas barrier in rat lungs

Zheng, Lu P., Rui Sheng Du, and Barbara E. Goodman.Effects of acute hyperoxic exposure on solute fluxes across the blood-gas barrier in rat lungs. J. Appl.Physiol. 82(1): 240-247, 1997.We investigatedeffects of acute hyperoxia on solute transport from air space tovascular space in isolated rat lungs. Air spaces were filled withKrebs-Ringer bicarbonate solution containing fluoresceinisothiocyanate-labeled dextran (FD-20; mol wt 20,000) and either²²Na⁺and [¹⁴C]sucrose, orD-[¹⁴C]glucoseandL-[³H]glucose.Apparent permeability-surface area products for tracers over time (upto 120 min) were calculated for isolated perfused lungs from controlrats (room air) and rats exposed to >95%O₂ for 48 or 60 h immediatelypostexposure. After O₂ exposures,mean fluxes for[¹⁴C]sucrose and FD-20were significantly higher than in room-air control lungs. However,amiloride-sensitive Na⁺ and activeD-glucose fluxes were unchangedafter hyperoxic exposure. Therefore, it is unlikely that decreases innet solute transport in this lung-injury model contributed to pulmonaryedema resulting from O₂ toxicity.Increased net solute transport shown to help resolve pulmonary edemaafter acute hyperoxic exposure must therefore begin during the recoveryperiod. In summary, our data show increases in passive solute fluxesbut no changes in active solute fluxes immediately after acutehyperoxic lung injury.

     

我国优秀划船运动员肱骨X线测量分析

本文对全国优秀划船运动员进行了肱骨X线摄片与测量,同时,将划船运动员与非运动员以及划船运动各专项运动员分别进行了对比。结果表明:划船运动,尤其是划艇专项训练对肱骨的生长发育具有良好的影响。本文资料可作为体育训练及运动员选材的参考。