全文获取类型
收费全文 | 1084篇 |
免费 | 101篇 |
国内免费 | 96篇 |
出版年
2024年 | 7篇 |
2023年 | 24篇 |
2022年 | 67篇 |
2021年 | 89篇 |
2020年 | 50篇 |
2019年 | 62篇 |
2018年 | 65篇 |
2017年 | 38篇 |
2016年 | 62篇 |
2015年 | 79篇 |
2014年 | 61篇 |
2013年 | 68篇 |
2012年 | 86篇 |
2011年 | 72篇 |
2010年 | 37篇 |
2009年 | 42篇 |
2008年 | 41篇 |
2007年 | 47篇 |
2006年 | 46篇 |
2005年 | 45篇 |
2004年 | 38篇 |
2003年 | 38篇 |
2002年 | 26篇 |
2001年 | 8篇 |
2000年 | 7篇 |
1999年 | 17篇 |
1998年 | 12篇 |
1997年 | 13篇 |
1996年 | 8篇 |
1995年 | 4篇 |
1994年 | 9篇 |
1993年 | 2篇 |
1992年 | 3篇 |
1991年 | 3篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1982年 | 2篇 |
排序方式: 共有1281条查询结果,搜索用时 15 毫秒
51.
Li Yuanbin Liu Haifen Zeng Zhaohui Lin Hui Chen Xin Yuan Xianglian Qiu Jizhe Fu Fengchun Chen Zhuang Kuang Jianjun 《Journal of molecular histology》2022,53(4):763-772
Journal of Molecular Histology - We investigate the protective effect of ginsenoside Rb3 on skin flap microvasculature following ischemia-reperfusion (I/R) injury and its regulatory mechanism. We... 相似文献
52.
Liu Yingjie Wang Jieyu Wang Lu Zhang Cheng Bai Qingxiang Lyu Xiaomei Yang Ruijin 《Phytochemistry Reviews》2022,21(5):1605-1626
Phytochemistry Reviews - Salidroside is a precious phenylethanoid glycoside derived from Rhodiola genus plants, which possesses a broad spectrum of biological properties for application in the... 相似文献
53.
Rui Sun Mengge Lyu Shuang Liang Weigang Ge Yingrui Wang Xuan Ding Cheng Zhang Yan Zhou Shanjun Chen Lirong Chen Tiannan Guo 《Proteomics》2022,22(7):2100147
Prostate cancer is the most common cancer in males worldwide. Mass spectrometry-based targeted proteomics has demonstrated great potential in quantifying proteins from formalin-fixed paraffin-embedded (FFPE) and (fresh) frozen biopsy tissues. Here we provide a comprehensive tissue-specific spectral library for targeted proteomic analysis of prostate tissue samples. Benign and malignant FFPE prostate tissue samples were processed into peptide samples by pressure cycling technology (PCT)-assisted sample preparation, and fractionated with high-pH reversed phase liquid chromatography (RPLC). Based on data-dependent acquisition (DDA) MS analysis using a TripleTOF 6600, we built a library containing 108,533 precursors, 84,198 peptides and 9384 unique proteins (1% FDR). The applicability of the library was demonstrated in prostate specimens. 相似文献
54.
55.
果生刺盘孢侵染危害多种植物,是重要的植物病原真菌。在一些丝状真菌中,敲除非同源末端连接修复通路的关键基因ku70或ku80可显著提高同源重组效率,进而提高靶基因置换频率。本研究从果生刺盘孢基因组鉴定到Cfku70和Cfku80两个基因,并明确了基因失活对菌株生物学表型和基因敲除效率的影响。敲除Cfku70或Cfku80不影响菌株的菌落形态、营养生长、产孢、分生孢子萌发、侵染结构发育和致病;Cfku70基因敲除还大幅提升3个测试基因的敲除效率。本研究证实Cfku70基因失活能显著提高果生刺盘孢的基因敲除效率,适宜作为高效基因敲除的底盘菌株,研究结果为通过批量敲除策略筛选新型致病因子奠定重要基础。 相似文献
56.
云南省作为中国生物多样性最高的省份, 其详实的物种本底资料对我国生物多样性研究和保护具有重要意义。本文在前期研究的基础上, 结合实体标本, 汇总编制了云南省现生、原生爬行动物更新名录。截至2021年12月31日, 云南省记录爬行动物25科82属235种, 其中龟鳖目4科12属16种, 有鳞目蜥蜴亚目6科20属72种, 蛇亚目15科50属147种。较《云南两栖爬行动物》确认新增82种, 存疑收录21种, 移除23种。基于先前云南省爬行动物区划和更新后的物种分布信息, 将云南省爬行动物地理分为6个动物地理区, 即滇西北横断山区、滇西山地区、滇南山地区、滇东南山地区、滇中高原区以及滇东北山地区; 其中滇西北横断山区、滇西山地区、滇中高原区和滇东南山地区的范围与先前研究相比有所调整。结合调整后的爬行动物地理区划, 对物种分布、物种特有性、受威胁状况等给出了统计结果。云南省爬行动物特有物种、国内仅见于云南的非特有物种数量较多, 受威胁等级高。建议今后继续加大分类学研究投入, 对滇西北、滇中特有爬行动物分布集中的区域积极开展栖息地保护工作, 同时在最新调整的《国家重点保护野生动物名录》基础上, 定期组织专家研讨, 对《云南省省级重点保护动物名录》提出更新建议。 相似文献
57.
Paecilomyces variotii extracts (ZNC) enhance plant immunity and promote plant growth 总被引:1,自引:0,他引:1
Lu Chongchong Liu Haifeng Jiang Depeng Wang Lulu Jiang Yanke Tang Shuya Hou Xuwen Han Xinyi Liu Zhiguang Zhang Min Chu Zhaohui Ding Xinhua 《Plant and Soil》2019,445(1-2):383-396
Plant and Soil - There are growing concerns regarding the restoration of karst rocky desertification (KRD) areas. However, the soil conditions and its residing microorganisms, which are essential... 相似文献
58.
Nian Wei Yan Zhang Fan Wu Ziwei Shen Huijun Ru Ling Liu Zhaohui Ni 《Zeitschrift fur angewandte Ichthyologie》2019,35(3):789-792
The length–weight relationships (LWRs) for 15 fish species from the Three Gorges Reservoir (TGR) were described in this paper. Specimens were collected using gill nets (mesh size: 6, 8, 10, 12 cm), cage net (mesh size: 1 cm), benthic fyke net (mesh size: 1 cm), hook longlines (80 m long, 3 barbless hooks per meter line) and electrofishing (power: 1,500 w; distance of electrodes: 1–2 m; water depth: 0.2–1.0 m; water area: 1–2 m2; catch by dip net [mesh size: 0.4 cm]) at four sections of the TGR, monthly in June–August and October–November in 2017 and 2018. The first LWR reference for Zacco acutipinnis (Bleeker, 1871), Discogobio brachyphysallidos Huang, 1989, Squalidus nitens (Günther, 1873), Leptobotia pellegrini Fang, 1936, Sinibotia reevesae (Chang, 1944), Pseudobagrus tenuifurcatus (Nichols, 1931), Pseudobagrus medianalis (Regan, 1904), Liobagrus marginatoides (Wu, 1930), Glyptothorax fokiensis (Rendahl, 1925), and Ctenogobius szechuanensis (Liu, 1940) and new maximum total length for 12 species are provided. 相似文献
59.
Tao Zhong Jianmei Zhang Xiaoying Han Xiaoxiao Gongye Tianqi Lyu Menghan Jiang Kaiwei Yu Xiaoqian Meng Dong Cheng Hui Lyu Tianliang Zhang Lei Zhang Shuzhen Liu 《Journal of cellular biochemistry》2019,120(9):15337-15346
3,3′,4,4′,5-Polychlorinated biphenyl (PCB126) is a persistent organic environmental pollutant which can affect various biological activities of organisms, such as immunity, neurological function, and reproduction. In our study, we aimed to investigate the effects of PCB126 on granulosa cells (GCs). GCs were collected from ovaries in PMSG-treated mice, after 24 hours culture. GCs were then incubated with 10 pg/mL, 100 pg/mL, and 10 ng/mL of PCB126 for another 24 hours. Following these steps, exposed GCs were collected for further experimentation. Our data showed that the number of GCs in the 10 ng/mL PCB126 decreased. Meanwhile, pyknotic nuclei and condensed chromatin increased, while the apoptotic cells in the 10 ng/mL PCB126 group were significantly increased. Furthermore, the expression of the apoptotic executive protein caspase-3 increased after PCB126 treatment. The expression of Bax, Bcl-2, and Bim related to the mitochondrial apoptosis pathway were also influenced to different degrees. Thus, our data suggested that PCB126 affect the GCs apoptosis, and mitochondrial apoptosis pathway was involved in this process. 相似文献
60.
Shanglun Xie Shanshan Han Zhen Qu Fei Liu Jingzhen Li Shanshan Yu James Reilly Jiayi Tu Xiliang Liu Zhaojing Lu Xuebin Hu Tinsae Assefa Yimer Yayun Qin Yuwen Huang Yuexia Lv Tao Jiang Xinhua Shu Zhaohui Tang Mugen Liu 《生物化学与生物物理学报:疾病的分子基础》2019,1865(6):1273-1283
Mutations in the photoreceptor cell-specific nuclear receptor gene Nr2e3 increased the number of S-cone photoreceptors in human and murine retinas and led to retinal degeneration that involved photoreceptor and non-photoreceptor cells. The mechanisms underlying these complex phenotypes remain unclear. In the hope of understanding the precise role of Nr2e3 in photoreceptor cell fate determination and differentiation, we generated a line of Nr2e3 knockout zebrafish using CRISPR technology. In these Nr2e3-null animals, rod precursors undergo terminal mitoses but fail to differentiate as rods. Rod-specific genes are not expressed and the outer segment (OS) fails to form. Formation and differentiation of cone photoreceptors is normal. Specifically, there is no increase in the number of UV-cone or S-cone photoreceptors. Laminated retinal structure is maintained. After normal development, L-/M-cones selectively degenerate, with progressive shortening of OS that starts at age 1 month. The amount of cone phototransduction proteins is concomitantly reduced, whereas UV- and S-cones have normal OS lengths even at age 10 months. In vitro studies show Nr2e3 synergizes with Crx and Nrl to enhance rhodopsin gene expression. Nr2e3 does not affect cone opsin expression. Our results extend the knowledge of Nr2e3's roles and have specific implications for the interpretation of the phenotypes observed in human and murine retinas. Furthermore, our model may offer new opportunities in finding treatments for enhanced S-cone syndrome (ESCS) and other retinal degenerative diseases. 相似文献