Enhanced expression of group II phospholipase A2 in human hepatocellular carcinoma

Enzyme activity, protein contents, and mRNA contents of group II phospholipase A₂ (PLA₂) in hepatocellular carcinoma (HCC) surgically obtained from 8 patients were compared with those in either its neighboring liver tissues or control liver tissues. The PLA₂ specific activity towards the mixed micelles of 1-palmitoyl-2-oleoyl-phosphatidylglycerol and cholate was significantly greater in the tumor tissues (6.62 ± 1.46 nmol/min/mg) than those in the surrounding liver tissues (1.33 ± 0.22 nmol/min/mg) and controls (0.43 ± 0.04 nmol/min/mg). The results of immunoblot analysis using a specific anti-human group II PLA₂ antibody and of Northern blot analysis using a human group II PLA₂ cDNA as a probe demonstrated that group II PLA₂ was responsible for the increased enzyme activity. The contents of immunoreactive group II PLA₂ in the tumor tissues (8.81 ± 1.24 ng/mg) were significantly higher than those in the surrounding liver tissues (1.77 ± 0.27 ng/mg); those in the control tissues were below the analytical range of the method used. The group II PLA₂ mRNA was also significantly increased in the tumor tissues, compared with that in the surrounding liver tissues, whereas it was not detectable in th controls. This indicates that group II PLA₂ in HCC is induced at the pretranslational level.     

马蹄香大小孢子发生及雌雄配子体形成

马蹄香（Ｓａｒｕｍａｈｅｎｒｙｉ,Ｏｌｉｖ．）花药壁的发育属双子叶型。花粉母细胞减数分裂为同时型,四分体主要为四面体形,少数为左右对称式排列。腺质绒毡层,其细胞可排列为不规则的两层,双核或多核。到单细胞花粉阶段,绒毡层细胞内切向壁上出现许多乌氏体。成熟花粉为２细胞型,圆球状,具单萌发沟。雌蕊６心皮,上部彼此分离、下部联合。倒生胚珠,双珠被,厚球心。胚囊发育蓼型。成熟胚囊为七细胞结构,但两个助细胞退化较早。     

长白山站的研究数据库管理系统

长白山站的研究数据库管理系统翟永华,赵士洞（中国科学院沈阳应用生态研究所１１００１５）Ｄａｔｅ－ＢａｓｅＭａｎａｇｅｍｅｎｔＳｙｓｔｅｍｉｎＣｈａｎｇｂａｉｓｈａｎＦｏｒｅｓｔＥｃｏｓｙｓｔｅｍＲｅｓｅａｒｃｈＳｔａｔｉｏｎ．￥ＺｈａｉＹｏｎｇｈｕａ...     

铅锌矿尾矿场植被重建的生态学研究Ⅰ.尾矿对种子萌发的影响

在实验条件下,供试的林木、农作物及牧草种子均能在铅锌矿尾矿中萌发,其中萌发率与非污染土壤中的相近,多数种子的萌发速率低于对照组。供试植物幼苗出土30天内没有死苗现象,但与对照相比,株高下降28.4%,复叶数减少34.1%,生物量下降52.6%.     

Plant regeneration from callus and explants of Sesbania spp.

Root, hypocotyl and cotyledon explants of Sesbania bispinosa, Sesbania cannabina, Sesbania formosa, and Sesbania sesban were cultured on Murashige and Skoog medium with benzyladenine (BA; 2.22, 4.44, 8.88 M) in combination with 2,4-dichlorophenoxyacetic acid (2,4-d; 2.26, 4.52, 9.05 M), indolebutyric acid (IBA; 0.25, 0.49, 4.92 M) or naphthaleneacetic acid (NAA; 2.69, 5.37, 10.74 M). Although all explant types developed some callus, callus occurred earliest and continued to grow fastest with hypocotyls. Media including 2.4-d or NAA gave the fastest growing callus. Callus was subcultured up to 10 times at 20-day intervals and retained a rapid growth rate. Shoots regenerated readily from both hypocotyls or cotyledons but not from roots. Shoot organogenesis was most frequent with IBA (0.25–4.92 M) in combination with BA (4.44–8.88 M) and did not occur with 2,4-d. With each species at least one medium induced shoot differentiation from more than 50 percent of the callus pieces. With one exception, media containing IBA that induced shoot organogenesis on explants also did so in callus, but media containing NAA, even when effective with explants, did not cause differentiation of callus. Shoots that differentiated were excised and cultured on MS medium without growth regulators or with IBA (2.46, 4.92, 9.84 M). Roots developed after 3–8 days on an appropriate rooting medium, often without IBA. Rooted plantlets were transplanted to pots in a greenhouse and developed into normal plants. Suitable media and protocols for initiating and subculturing callus and regenerating whole plants in vitro from callus and explants have thus been established for four species of Sesbania.     

新疆的刺球座属、穴壳属及普氏腔孢属

本文报道了新疆腔菌纲座囊菌目刺球座属(Lasiobotrys)、穴壳属(Dothiora)和普氏腔孢属(Plowrightia)的六种子囊菌,即:忍冬刺球座菌(L.loniccrae)、花楸穴壳菌 (D.sorbi)及其无性阶段花楸疡壳孢(Dothichiza sorbi)、茶蔗子普氏腔孢菌(P.ribesia)、小檗普氏腔孢菌(P.berberidiJ)、沙棘普氏腔孢菌(P.hippophaeos)及雕刻普氏腔孢菌(P.insculpta)。这三个属的真菌在我国均未报道过,为我国新纪录属(种)。标本均采于新疆,保存于新疆八一农学院植保系真菌标本室(HMAAC)。     

重组人IL6／2融合蛋白对6.5Gy照射小鼠造血功能恢复的影响

观察了ｈＦＰＩＬ６／２对６．５Ｇｙγ线照射ＮＩＨ小鼠第１０天造血功能恢复的影响。结果表明：照射小鼠连续４ｄ给予ｈＦＰＩＬ６／２２５０μｇ·ｋｇ－１·ｄ－１,其脾重、ＣＦＵ－８、骨髓有核细胞数及ＣＭ－ＣＦＵ分别比对照组增加５９．０％、２７８．５％、５７．９％和１３８．２％,统计学处理均有显著差异;对此四项指标的改善也明显优于２５μｇ组。另外,２５０μｇ剂量组小鼠外周血象３０ｄ的动态观察结果表明,ｈＦＰＩＬ６／２不但能明显提高红细胞和血红蛋白的最低值,而且能使血小板的恢复提前。提示ｈＦＰＩＬ６／２在促进血小板生成和促进红系造血方面可能具有良好的应用前景。     

尼群地平对实验性糖尿病大鼠心肌收缩性的影响

腹腔注射链脲佐霉素（６５ｍｇ／ｋｇ）诱发Ｗｉｓｔａｒ大鼠糖尿病。糖尿病发病４周后,向饲料中加尼群地平（３０ｍｇ·ｋｇ－１·ｄ－１）。结果表明,糖尿病４周时大鼠心室舒张功能首先受损,８周后心室舒张和收缩功能均明显受累。尼群地平处理对糖尿病大鼠的心肌收缩性有一定的改善作用。提示尼群地平对大鼠糖尿病性心肌病有一定有益作用。     

茶叶中总硒含量及其影响因素的研究

茶叶中硒素总量测定结果表明：土壤含硒量的高低是直接影响茶叶中硒的总量。茶树根、茎、叶、果中均有硒元素,叶片是茶硒积累的主要器官,尤其是老叶,其含量是嫩叶的几倍．茶树品种间含量的差异显著,最大差异达10倍以上．毛茶加工的成品茶含硒量受加工技术措施影响较大,其不同等级的含硒量与级别没有线性关系．     

河南木兰属9种植物过氧化物同工酶分析

本文采用聚丙烯酰胺凝胶电泳系统,测定了河南木兰属8种、1变种50多份的成熟叶片材料的过氧化物同工酶酶谱。测定结果表明,该属植物9种、变种的酶谱均有差异性,每种、变种均有特征酶谱,种间酶谱显著大于种内酶谱,根据其酶谱差异性,可以进行生物种、变种的鉴别和良种的选择。为免除其酶带Rf单一指标,鉴别生物种、变种可能出现的问题,作者创立了"酶谱多指标综合判断距离法"分析技术,首次将该属植物种、变种酶谱的酶带数目、Rf、酶带宽度及其活性强弱4个因子的差异性,进行编码联机、电脑运算分析,其结果与该属玉兰亚属内的玉兰派和辛夷派的形态分类相吻合。但是,从酶学观点不支持椭圆叶玉兰作为独立种的存在,以作为河南玉兰的变种为宜,也不支持河南玉兰派和腋花玉兰派的成立,以并入辛夷派为佳。