全文获取类型
收费全文 | 2389篇 |
免费 | 188篇 |
国内免费 | 175篇 |
专业分类
2752篇 |
出版年
2024年 | 11篇 |
2023年 | 35篇 |
2022年 | 88篇 |
2021年 | 129篇 |
2020年 | 122篇 |
2019年 | 129篇 |
2018年 | 108篇 |
2017年 | 100篇 |
2016年 | 109篇 |
2015年 | 138篇 |
2014年 | 172篇 |
2013年 | 177篇 |
2012年 | 205篇 |
2011年 | 199篇 |
2010年 | 108篇 |
2009年 | 112篇 |
2008年 | 96篇 |
2007年 | 100篇 |
2006年 | 100篇 |
2005年 | 68篇 |
2004年 | 57篇 |
2003年 | 58篇 |
2002年 | 42篇 |
2001年 | 35篇 |
2000年 | 33篇 |
1999年 | 28篇 |
1998年 | 20篇 |
1997年 | 23篇 |
1996年 | 18篇 |
1995年 | 17篇 |
1994年 | 24篇 |
1993年 | 12篇 |
1992年 | 16篇 |
1991年 | 13篇 |
1990年 | 7篇 |
1989年 | 7篇 |
1988年 | 9篇 |
1987年 | 8篇 |
1986年 | 4篇 |
1985年 | 7篇 |
1984年 | 1篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1979年 | 3篇 |
排序方式: 共有2752条查询结果,搜索用时 0 毫秒
51.
52.
塔克拉玛干沙漠腹地胡杨林土壤细菌多样性分析 总被引:2,自引:0,他引:2
【目的】对塔克拉玛干沙漠腹地胡杨林土壤细菌多样性进行初步探索,为下一步从中筛选可用于生物饲料或生物肥料的微生物奠定基础。【方法】采用可培养方法,进行细菌的分离纯化。对各菌株进行革兰氏染色及淀粉酶、酯酶、纤维素酶和NaCl耐受浓度的测定,并提取各菌株基因组DNA,进行16SrRNA基因扩增、测序及系统进化树的绘制,分析其多样性。【结果】共分离得到27株菌,其中放线菌门(Actinobacteria)16株,变形菌门(Proteobacteria)4株,厚壁菌门(Firmicutes)6株,拟杆菌门(Bacteroidetes)1株。革兰氏染色结果表明,5株菌为革兰氏阴性,其余为革兰氏阳性;酶活测定结果表明,15株菌具有淀粉酶活性,9株菌具有酯酶活性,9株菌具有纤维素酶活性;NaCl耐受浓度测定结果显示,NaCl浓度为2%时所有菌株均能生长,5%时能生长的有22株,15%时能生长的有1株。【结论】塔克拉玛干沙漠腹地胡杨林土壤中存在较丰富的细菌类群,且具有一定的酶学活性和NaCl耐受性,具有进一步研究开发的价值。 相似文献
53.
目的:在大肠杆菌中表达肠出血性大肠杆菌(EHEC)毒力岛上的毒力因子Z1444并纯化,对其丝/苏氨酸激酶活性进行初步检测。方法:根据GenBank中Z1444基因序列及pET-28a(+)载体的多克隆位点设计引物,以EHECO157∶H7全菌裂解液为模板,经PCR钓取1047 bp的目的片段,与表达载体pET-28a(+)连接,构建重组表达质粒pET-28a(+)-Z1444,将其转化至大肠杆菌BL21(DE3)中,IPTG诱导蛋白表达并经SDS-PAGE鉴定,利用体外反应体系鉴定重组蛋白的丝/苏氨酸激酶活性。结果:双酶切和测序鉴定表明,pET-28a(+)-Z1444原核表达质粒构建正确;诱导表达后经纯化,获得纯度在90%以上的可溶性重组Z1444,相对分子量约为38×103;体外酶活实验验证了Z1444的丝/苏氨酸激酶活性。结论:Z1444在大肠杆菌中获得高效可溶性表达,为后续功能验证奠定了基础。 相似文献
54.
Trivedi CM Luo Y Yin Z Zhang M Zhu W Wang T Floss T Goettlicher M Noppinger PR Wurst W Ferrari VA Abrams CS Gruber PJ Epstein JA 《Nature medicine》2007,13(3):324-331
In the adult heart, a variety of stresses induce re-expression of a fetal gene program in association with myocyte hypertrophy and heart failure. Here we show that histone deacetylase-2 (Hdac2) regulates expression of many fetal cardiac isoforms. Hdac2 deficiency or chemical histone deacetylase (HDAC) inhibition prevented the re-expression of fetal genes and attenuated cardiac hypertrophy in hearts exposed to hypertrophic stimuli. Resistance to hypertrophy was associated with increased expression of the gene encoding inositol polyphosphate-5-phosphatase f (Inpp5f) resulting in constitutive activation of glycogen synthase kinase 3beta (Gsk3beta) via inactivation of thymoma viral proto-oncogene (Akt) and 3-phosphoinositide-dependent protein kinase-1 (Pdk1). In contrast, Hdac2 transgenic mice had augmented hypertrophy associated with inactivated Gsk3beta. Chemical inhibition of activated Gsk3beta allowed Hdac2-deficient adults to become sensitive to hypertrophic stimulation. These results suggest that Hdac2 is an important molecular target of HDAC inhibitors in the heart and that Hdac2 and Gsk3beta are components of a regulatory pathway providing an attractive therapeutic target for the treatment of cardiac hypertrophy and heart failure. 相似文献
55.
Zheng-ke Zhan Kun-mei Ji Xiao-yu Liu Zhi-gang Liu Meng Li Jia-jie Chen Jia-na Li Shi Qiu 《Experimental & applied acarology》2010,52(1):63-71
Home dust mite derived materials are known to be a major source of problematic inhalant allergens. The aim of this study was
to determine the localization of the group 3 allergen, Der f 3, within Dermatophagoides farinae, in order to assess the relative importance of excreted materials and nonexcreted body components as allergen sources. Recombinant
Der f 3 (rDer f 3) was expressed in bacteria and purified as an immunogen for production of monoclonal antibodies (mAb) against
it. Dermatophagoides farinae mites and their faecal pellets were embedded in paraffin, and serial sections were immunoprobed with mAb clone 3D3 against
Der f 3. D. farinae midgut mucosa, gut contents and faecal pellets were strongly immunopositive for Der f 3. Der f 3 immunoreactive products
were not detected in any other internal organs of the mite. These results suggest that Der f 3 allergen may be synthesized
in and secreted from the digestive tract and excreted from the mite’s body in the faecal pellets. 相似文献
56.
Su Li Dongsheng Zhang Jian Sun Zhinming Li Liting Deng Benyan Zou Jing Zhan Wenqi Jiang 《MABS-AUSTIN》2012,4(2):256-266
The safety and pharmacokinetics assessment of antibodies targeting CD22 (e.g., epratuzumab) have been established in western Caucasian populations, but there are no reports of the effects in Chinese populations. This dose-escalation study examines the safety, pharmacokinetics and biologic effects of multiple doses of anti-CD22 human-murine chimeric monoclonal antibody SM03 in 21 Chinese patients with CD22-positive non-Hodgkin lymphoma. Most of drug-related adverse events (AEs) were mild and reversible. Two patients experienced serious AEs (hemorrhage); one patient had grade 4 neutropenia; one patient had asymptomatic grade III prolongation of activated partial thromboplastin time (APTT). Major AEs included fever (71%), prolongation of APTT (42.8%), leukocytopenia (44.4%), alanine transaminase elevation (28.6%), elevated serum creatinine (23.8%) and injection site skin redness (14.3%). Circulating B cells transiently decreased without significant effects on T cells or immunoglobulin levels. Pharmacokinetic data revealed that mean maximum observed SM03 concentration and mean AUC from time zero to infinity increased in a dose-dependent manner up to 360 mg/m2 SM03. Mean clearance was similar at doses ≤360 mg/m2 and decreased significantly at dose 480 mg/m2, supporting saturation of B-cell binding at 360 mg/m2. Across all dose levels and histologies, one patient achieved partial response at 480 mg/m2 dose; 14 patients had stable disease as best response and four patients progressed. Overall, SM03 was tolerated at doses ranging from 60–480 mg/m2 and had potential efficacy in Chinese patients with follicular lymphoma. 相似文献
57.
Activation of phagocytic NADPH oxidase requires association of its cytosolic subunits with the membrane-bound flavocytochrome. Extensive phosphorylation of the p47(phox) subunit of NADPH oxidase marks the initiation of this activation process. The p47(phox) subunit then translocates to the plasma membrane, bringing the p67(phox) subunit to cytochrome b558 to form the active NADPH oxidase complex. However, the detailed mechanism for targeting the p47(phox) subunit to the cell membrane during activation still remains unclear. Here, we show that the p47(phox) PX domain is responsible for translocating the p47(phox) subunit to the plasma membrane for subsequent activation of NADPH oxidase. We also demonstrate that translocation of the p47(phox) PX domain to the plasma membrane is not due to interactions with phospholipids but rather to association with the actin cytoskeleton. This association is mediated by direct interaction between the p47(phox) PX domain and moesin. 相似文献
58.
Peiyong Guo Ding Lu Jiaying Jiang Liye Zhan Ruoshi Wang 《Chemical Speciation and Bioavailability》2017,29(1):170-178
In this study, the fractionation and distribution of phosphorus (P) in the core sediments of the Shanmei reservoir were investigated by using the chemical extraction method in directions for the first time in order to understand its bio-availability, adsorption characteristics, potential release and environmental significance. The results of the study showed that P in the sediments mainly consisted of inorganic phosphorus (IP) and that IP mainly consisted of non-apatite phosphorus (NAIP). The horizontal and temporal distributions of the P fractions were different from each other, but the vertical distribution was similar, which indicated a trend of stabilization after falling. The content of total phosphorus (TP), IP, organic phosphorus (OP), NAIP, apatite phosphorus (AP), and bio-available phosphorus (BAP) in the sediments during the three seasons ranged from 193.85 to 1664.05 mg·kg?1, 126.90 to 1127.70 mg·kg?1, 43.74 to 669.29 mg·kg?1, 57.62 to 937.07 mg·kg?1, 32.58 to 250.71 mg·kg?1, and 41.06 to 871.82 mg·kg?1, respectively. NAIP contents in the sediments accounted for more than 50% of TP. Using an analysis from three aspects, the eutrophication risk index (ERI) could be used to assess the potential release of P in the sediments, and there was a high release risk of P in the sediments in the Shanmei reservoir. 相似文献
59.
60.
Haemocyte types of the scallop (Chlamys farreri) were identified by Giemsa stain and flow cytometry (FCM). Additionally, the activities of peroxidase (POD), phenoloxidase (PO) and alkaline phosphatase (ALP) in haemocytes were analysed by immunocytochemical and biochemical methods. The results indicate that there were two types of haemocytes in the scallop, hyalinocytes and granulocytes, and that POD, PO and ALP were more abundant and more active in granulocytes than in hyalinocytes. 相似文献