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291.
Bo‐Fei Chen Mark Liu Dustin R. Rubenstein Syuan‐Jyun Sun Jian‐Nan Liu Yu‐Heng Lin Sheng‐Feng Shen 《Ecology letters》2020,23(3):467-475
Although interspecific competition has long been recognised as a major driver of trait divergence and adaptive evolution, relatively little effort has focused on how it influences the evolution of intraspecific cooperation. Here we identify the mechanism by which the perceived pressure of interspecific competition influences the transition from intraspecific conflict to cooperation in a facultative cooperatively breeding species, the Asian burying beetle Nicrophorus nepalensis. We not only found that beetles are more cooperative at carcasses when blowfly maggots have begun to digest the tissue, but that this social cooperation appears to be triggered by a single chemical cue – dimethyl disulfide (DMDS) – emitted from carcasses consumed by blowflies, but not from control carcasses lacking blowflies. Our results provide experimental evidence that interspecific competition promotes the transition from intraspecific conflict to cooperation in N. nepalensis via a surprisingly simple social chemical cue that is a reliable indicator of resource competition between species. 相似文献
292.
Tao Zhang Zhihua Feng Chunle Luo Yixin Sun Jinzhen Li Juntian Xu Xuchen Wang 《Journal of Plant Ecology》2020,13(5):525
盐沼植被是沿海水体中溶解有机物(Dissolved Organic Matter, DOM)的重要贡献者。然而,不同盐沼植物释放DOM的动力学特征尚缺乏系统研究和比较。黄河三角洲湿地是中国东海岸面积最大,保护最完善的沿海生态系统之一。本论文研究了2016年10月从黄河三角洲(Yellow River Delta, YRD)盐沼中采集的三种常见沼泽植物(芦苇(Phragmites australis),碱蓬(Suaeda salsa)和獐茅(Aeluropus littoralis)的DOM释放过程。通过测定溶解有机碳(Dissolved Organic Carbon, DOC)和溶解氮(Dissolved Nitrogen, DN)发现,植物叶片释放的DOM浓度远高于其根和茎。在27天的培养期内,平均有15%的生物碳和30%的生物氮以DOC和DN的形式通过植物叶片释放。从植物中释放的DOM非常不稳定,在27天的培养期内,细菌共消耗了92.4%–98.1%的DOC和88.0%–94.6%的DN。植物释放DOM的荧光特性表明,发色溶解的有机物(Chromophoric Dissolved Organic Matter, CDOM)是DOM的主要组分,而类蛋白组分是植物释放CDOM的主要组分。细菌的降解作用改变了DOM的荧光性质和化学组成。上述的室内研究结果得到了实地调查的充分支撑,表明在深秋时期黄河口湿地有大量DOM溢出。本研究结果表明,盐沼植物释放的DOM是沼泽和沿海水域DOC和DN的重要来源,而且易降解DOC和DN为黄河口湿地和邻近沿海水域中的微生物群落提供了重要的食物来源。 相似文献
293.
Jun Mi Yang Feng Jie Wen Yiqun Su Lin Xu Tingjian Zu Chang Liu David E. Fisher Xunwei Wu 《Pigment cell & melanoma research》2020,33(1):16-29
Primary melanocytes isolated from skin and expanded in culture have been widely used for laboratory research and clinical applications. The conventional method to isolate primary melanocytes from skin usually requires about 3–4 weeks of culture for melanocytes to grow sufficiently to passage. Considering that melanocytes comprise only 3%–7% of epidermal cells in normal human skin, it would be extremely helpful to increase the isolation efficiency and shorten the initial culture time to quickly meet various application needs. Here, we report that adding Y‐27632, a Rho kinase inhibitor, into the initial culture medium for 2 days can dramatically increase the yield of melanocytes. We found that Y‐27632 can promote keratinocyte attachment and survival in the melanocyte culture system, resulting in not only better recovery, but also increased proliferation of melanocytes by a paracrine signaling pathway. More specifically, Y‐27632 significantly induced keratinocyte expression of stem cell factor, which played an important role in enhancing the growth of melanocytes. In summary, Y‐27632 could profoundly enhance the yield of primary melanocytes in the initial culture through paracrine effects on keratinocytes. 相似文献
294.
Abdullah Al Emran Hsin‐Yi Tseng Mikaela C. Coleman Jessamy Tiffen Stuart Cook Helen M. McGuire Stuart Gallagher Carl Feng Peter Hersey 《Pigment cell & melanoma research》2020,33(5):660-670
Melanoma, as for many other cancers, undergoes a selection process during progression that limits many innate and adaptive tumor control mechanisms. Immunotherapy with immune checkpoint blockade overcomes one of the escape mechanisms but if the tumor is not eliminated other escape mechanisms evolve that require new approaches for tumor control. Some of the innate mechanisms that have evolved against infections with microorganisms and viruses are proving to be active against cancer cells but require better understanding of how they are activated and what inhibitory mechanisms may need to be targeted. This is particularly so for inflammasomes which have evolved against many different organisms and which recruit a number of cytotoxic mechanisms that remain poorly understood. Equally important is understanding of where these mechanisms will fit into existing treatment strategies and whether existing strategies already involve the innate killing mechanisms. 相似文献
295.
296.
Sufang Zhang Sifan Shen Jiong Peng Xin Zhou Xiangbo Kong Pingping Ren Fu Liu Lingling Han Shuai Zhan Yongping Huang Aibing Zhang Zhen Zhang 《Molecular ecology resources》2020,20(4):1023-1037
Dendrolimus spp. are important destructive pests of conifer forests, and Dendrolimus punctatus Walker (Lepidoptera; Lasiocampidae) is the most widely distributed Dendrolimus species. During periodic outbreaks, this species is said to make “fire without smoke” because large areas of pine forest can be quickly and heavily damaged. Yet, little is known about the molecular mechanisms that underlie the unique ecological characteristics of this forest insect. Here, we combined Pacific Biosciences (PacBio) RSII single‐molecule long reads and high‐throughput chromosome conformation capture (Hi‐C) genomics‐linked reads to produce a high‐quality, chromosome‐level reference genome for D. punctatus. The final assembly was 614 Mb with contig and scaffold N50 values of 1.39 and 22.15 Mb, respectively, and 96.96% of the contigs anchored onto 30 chromosomes. Based on the prediction, this genome contained 17,593 protein‐coding genes and 56.16% repetitive sequences. Phylogenetic analyses indicated that D. punctatus diverged from the common ancestor of Hyphantria cunea, Spodoptera litura and Thaumetopoea pityocampa ~ 108.91 million years ago. Many gene families that were expanded in the D. punctatus genome were significantly enriched for the xenobiotic biodegradation system, especially the cytochrome P450 gene family. This high‐quality, chromosome‐level reference genome will be a valuable resource for understanding mechanisms of D. punctatus outbreak and host resistance adaption. Because this is the first Lasiocampidae insect genome to be sequenced, it also will serve as a reference for further comparative genomics. 相似文献
297.
Lipopeptides are produced by nonribosomal peptide synthetases (NRPSs) and contain diverse fatty acyl moieties that are major determinants of antibiotic potency. The lipid chains are incorporated into peptidyl backbones via lipoinitiation, a process comprising free fatty acid activation and the subsequent starter condensation domain (C1)‐catalyzed conjugation of fatty acyl moieties onto the aminoacyl substrates. Thus, a thorough understanding of lipoinitiation biocatalysts would significantly expand their potential to produce novel antibiotics. Here, biochemical assays, in silico analysis, and mutagenesis studies are used to ultimately identify the specific amino acid residues that control the fatty acyl substrate selectivity of C1 in lipopeptide A54145. In silico docking study has identified four candidate amino acids, and subsequent in vitro assays confirmed their functional contribution to the channel that controls substrate selectivity. Two engineered variants with single point mutations in C1 are found to alter the substrate selectivity toward nonnatural fatty acyl substrates. The detailed mechanistic insights into the catalytic contribution of C1 obtained from the present study will facilitate future NPRS biocatalyst efforts 相似文献
298.
Zhifeng Huang Yingxiang Du Xiaoqi Li Zijie Feng Xiaodong Sun Xiaofei Ma Mingxuan Ma 《Chirality》2020,32(1):98-106
Transfersomes were a special kind of nanomaterials with higher deformability and flexibility. A rapid method for coated-column preparation using anionic transfersomes as a coating material by electrostatic adsorption was developed. With carboxymethyl-β-cyclodextrin added in running buffer as the chiral selector, the capillary electrochromatography enantioseparation system based on the transfersomes-coated column modified by electrostatic adsorption was established for the first time. Propranolol and metoprolol acted as model drugs to evaluate the enantioseparation performance, these two basic drugs achieved baseline separation with satisfactory resolution and selection factor in this transfersomes-electrochromatography system but only partial separation in bare column system. In order to get the optimal separation condition, concentration of chiral selector, buffer pH, and applied voltage were systematically investigated. A rapid and efficient enantioseparation electrochromatography system was established and showed that transfersomes as the stationary phase could efficiently improve chiral separation effect. 相似文献
299.
Hui Fang Xiuyi Fu Yuebin Wang Jing Xu Haiying Feng Weiya Li Jieting Xu Orawan Jittham Xuan Zhang Lili Zhang Ning Yang Gen Xu Min Wang Xiaowei Li Jiansheng Li Jianbing Yan Xiaohong Yang 《The Plant journal : for cell and molecular biology》2020,101(2):278-292
The nutritional traits of maize kernels are important for human and animal nutrition, and these traits have undergone selection to meet the diverse nutritional needs of humans. However, our knowledge of the genetic basis of selecting for kernel nutritional traits is limited. Here, we identified both single and epistatic quantitative trait loci (QTLs) that contributed to the differences of oil and carotenoid traits between maize and teosinte. Over half of teosinte alleles of single QTLs increased the values of the detected oil and carotenoid traits. Based on the pleiotropism or linkage information of the identified single QTLs, we constructed a trait–locus network to help clarify the genetic basis of correlations among oil and carotenoid traits. Furthermore, the selection features and evolutionary trajectories of the genes or loci underlying variations in oil and carotenoid traits revealed that these nutritional traits produced diverse selection events during maize domestication and improvement. To illustrate more, a mutator distance–relative transposable element (TE) in intron 1 of DXS2, which encoded a rate‐limiting enzyme in the methylerythritol phosphate pathway, was identified to increase carotenoid biosynthesis by enhancing DXS2 expression. This TE occurs in the grass teosinte, and has been found to have undergone selection during maize domestication and improvement, and is almost fixed in yellow maize. Our findings not only provide important insights into evolutionary changes in nutritional traits, but also highlight the feasibility of reintroducing back into commercial agricultural germplasm those nutritionally important genes hidden in wild relatives. 相似文献