miR-129-5p improves cardiac function in rats with chronic heart failure through targeting HMGB1

Mammalian Genome - Increasing evidence shows that miRNAs play pivotal roles in cardiovascular diseases, including heart failure (HF). The aim of this study was to investigate the role of miR-129-5p...     

不同生态型芦苇种群对盐胁迫的生长和光合特性

土壤盐渍化是影响我国土壤利用效率的主要因素之一,芦苇是改良土壤盐渍化的良好实验材料,但芦苇有着多种的生态型,比较各生态型芦苇的耐盐差异成为亟待解决的问题。通过设置淡水(0.00%)与加盐(质量浓度2.00%)处理控制实验,测量芦苇的生长指标和光合指标,比较河口型芦苇与内陆型芦苇耐盐性,寻找合适生态型的芦苇作为改良土壤盐渍化的生物材料。在实验中,与淡水条件相比,加盐(2.00%)处理条件下,河口型芦苇和内陆型芦苇的株高(height)、蒸腾速率(E)均显著性下降,但是两种生态型的芦苇的水分利用效率(WUE)明显提高;河口型的芦苇相对生长速率(RGR)和气孔导度(Gs)都明显高于内陆型芦苇。在淡水环境中,河口型芦苇的相对生长速率(RGR)和净光合速率(A)都显著性地高于内陆型芦苇。结果表明两种生态型的芦苇在进化过程中存在一定程度上的分化,盐胁迫会抑制两种芦苇的生长,两种生态型芦苇的相对生长速率和气孔导度在盐胁迫下出现明显地差异,表明两种生态型的芦苇对盐度的响应机制有所差异。相比于内陆型芦苇,河口型芦苇有着更强的耐盐性,内陆型及河口型芦苇的表型性状差异主要是由于其原生境的差异所决定的。     

Synthesis and evaluation of piperidine urea derivatives as efficacious 11β-hydroxysteroid dehydrogenase type 1 inhibitors in diabetic ob/ob mice

11β-Hydroxysteroid dehydrogenase type 1 (11β-HSD1) has attracted considerable attention as a potential target for the treatment of diabetes and metabolic syndrome. Herein we report the design, synthesis and efficacy evaluation of novel amide and urea 11β-HSD1 inhibitors. Structure-activity relationship studies led to the identification of 10c, which was efficacious in a diabetic ob/ob mouse model and reduced fasting and non-fasting blood glucose levels after ip dosing.     

Structure of a Pancreatic ATP-Sensitive Potassium Channel

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Development of a Loop-Mediated Isothermal Amplification Assay for Porcine Circovirus Type 2

In this study, the loop-mediated isothermal amplification (LAMP) method was used to develop a rapid and simple detection system for porcine circovirus type 2 (PCV2). According to the PCV2 sequences published in GenBank, multiple LAMP primers were designed targeting conserved sequences of PCV2. Using the DNA extracted from PCV2 isolates HUN-09 and SD-09 as the template, LAMP reactions in a PCV2 LAMP system was performed, the amplification products were detected by adding SYBR Green I and could be observed di...     

城镇化进程中居民生活消费的生态环境压力评估—— 以江苏省江阴市为例

消费问题是区域生态环境压力增大和生态环境问题产生的根本原因。提出人口环境消费的概念和理论模型,并在生态足迹理论和方法的支撑下分别计算江苏省江阴市1997～2004年城镇居民和农村居民生活消费的环境压力;采用SPSS14.0软件,分析城镇和农村居民这两类人群人均环境消费的变化规律及其影响因素,并通过建立多元线性回归预测模型,估算在城镇化快速发展的情况下江阴市人口环境消费期望值。研究表明：江阴市城镇居民人均环境消费从1997年的1.395hm^2上升到2004年的1.960hm^2,年均递增0.081hm^2;农村居民则从1997年的1.345hm^2上升到2004年的1.465hm^2,年均递增0.017hm^2。城乡居民人均环境消费与人均可支配收入、人均受教育年限、恩格尔系数、人均地区生产总值等社会经济指标呈显著线性相关。预计到2010年城乡居民人均环境消费将分别达到2.258hm^2和1.919hm^2,到2020年将分别达到2.807hm^2和2.303hm2;2010年和2020年区域生态赤字将比2004年分别增长55%和107%,给区域生态环境造成巨大压力。为有效缓解区域环境压力,论文提出合理控制区域人口规模和城镇化发展速度,大力发展生态产业,培养可持续消费观念,引导绿色、健康和环境友好型的消费模式等对策措施。     

鸡apoA5基因单核苷酸多态性及其与屠体性状的关联研究

以丝羽乌骨鸡和隐性白洛克正反交产生的F2代为实验群体, 采用PCR-SSCP和DNA测序的方法检测鸡载脂蛋白A5(apoA5)基因的单核苷酸多态性(SNPs), 并将所发现的SNPs与体重、胸肌重、腿肌重、心脏重、肝脏重和腹脂重等屠体性状进行关联分析。结果发现, 鸡apoA5基因5′-调控区C-169T, 外显子2 C600T、T635C, 外显子3 C841G、C914T、C1142G、C1394T共7个突变位点。其中外显子2突变位点C600T、T635C对12周龄腹脂重、腹脂率、肝脏重和心脏重有显著影响(P<0.05), 根据PCR-SSCP的结果将其分为6种基因型(AA, AB, AC, BB, BC, CC): 其中CC型个体的腹脂重和腹脂率显著高于AA型、AB型、AC型、BB型、BC型 (P<0.05); AC型个体的肝脏重显著低于AA型、AB型、BB型、BC型和CC型的肝脏重(P<0.05); BC型个体的心脏重显著低于BB型的心脏重(P<0.05)。     

Agmatine and Imidazoline Receptors: Their Role in Opioid Analgesia, Tolerance and Dependence

Agmatine is an endogenous amine that is synthesized following the decarboxylation of L-arginine by arginine decarboxylase. Agmatine exists in mammalian brain and has been proposed as a neurotransmitter and/or neurotransmodulator. Agmatine binds to several targets and is considered as an endogenous ligand for imidazoline receptors. This review, mainly based on our research work in the past decade, focused on the modulations by agmatine action on imidazoline receptors to opioid analgesia, tolerance and dependence, and its possible neurochemical mechanisms. We went on to propose that agmatine and imidazoline receptors constitute a novel system of modulating opioid functions.     

荆条叶性状对野外不同光环境的表型可塑性

光照是影响植物生长和分布的重要环境因子。对生长在野外5种不同光环境下(林外、阔叶林林缘、阔叶林林下、针叶林林窗和针叶林林下)的荆条的叶片进行取样研究,通过对光合作用光响应曲线、叶绿素荧光、叶绿素含量、叶片氮磷含量以及叶片形态的测量,来反映荆条对不同光环境的表型可塑性。研究结果表明,荆条叶片对于野外不同的光环境具有很好的适应机制,叶片功能性状受到结构性状的调节。低光下通过高的比叶面积(SLA)、单位质量叶绿素含量、光系统II最大量子产量,低的暗呼吸速率、光饱和点、光补偿点、叶绿素a,b的比值来提高对光能的利用效率,维持生长;高光下则通过与SLA有关的叶片结构的变化对光合作用进行调节。大多数的叶性状只受到日光照总量的影响,SLA的大小与日最高光强有关,可以对不同日变化模式的光照做出迅速的响应,是适应不同光照的敏感指标。尽管光照是不同光环境下影响荆条叶性状的主要环境因子,土壤养分含量同样会对叶性状产生影响,高土壤养分下的高叶长与叶柄长的比值体现了植物对资源获取和支撑结构之间分配的权衡。