Ultrastructural and functional abnormalities of mitochondria in cultivated fibroblasts from α -mannosidosis patients

α-Mannosidosis is a lysosomal storage disorder caused by α-mannosidase deficiency. Clinical course of the disease ranges from severe infantile to milder juvenile type and includes mental retardation, skeletal deformities, coarse facies, hepatomegaly and hearing loss. The aim of the study was to analyse mitochondrial ultrastructure and function in cultivated fibroblasts from three patients with α-mannosidosis. All patients were homozygous for the c.2248C>T mutation in the MAN2B1 gene encoding lysosomal α-mannosidase. The mutation results in incorrect protein folding and severe decrease of α-mannosidase activity. The misfolded protein is retained by the control system of endoplasmic reticulum (ER). In analysed fibroblasts, we observed dilated ER, higher amount of aberrant mitochondria and reduced mitochondrial mass compared to controls. Respiratory chain complex IV, cytochrome c oxidase (COX), activity and the ratio between COX and citrate synthase (control enzyme) were significantly increased in comparison to controls (P < 0.05). Furthermore, the activity at least from one of other respiratory chain complexes was increased in each studied cell line. Mitochondrial membrane potential as well as reactive oxygen species production were comparable with controls. Based on our results, we hypothesize more profound effect of swelled and damaged mitochondria and ER dilatation on tissues with higher energy demand than fibroblasts have.     

罗非鱼绿色气球菌的鉴定及致病性研究

研究首次报道了从广东茂名市茂南区患病罗非鱼体内分离的两特殊细菌菌株Mnlv1 和Mnlv2, 通过细菌形态学、培养条件比较、生理生化特征及16S rRNA 基因检测等鉴定了该病原, 采用三种不同的感染方式对罗非鱼实施人工感染以期探讨该菌对罗非鱼的致病强度及致病条件, 同时也比较了两菌株对29 种不同的抗生素的敏感性差异。结果表明: 两菌株均为绿色气球菌(Aerococcus viridans), 革兰氏染色阳性(G+), 溶血性。感染实验结果显示, 该菌株对罗非鱼具有较强的致病性, 并且随着环境胁迫作用的加强, 鱼体发病死亡率增高。药敏试验结果显示, 两菌株为对头孢曲松、米诺四环素、诺氟沙星等15 种药物高度敏感, 对麦迪霉素、壮观霉素和新霉素3 种药物中度敏感, 对红霉素、乙酰螺旋霉素、苯唑青霉素等10 种药物不敏感。研究将为罗非鱼绿色气球菌病原的诊断及防治提供理论基础。       

三氯杀螨醇对草鱼和南美白对虾的急性毒性

为提高农作物产量,人类在较长时期内仍将依赖杀虫剂。种植业喷洒的化学杀虫剂可通过不同途径进入并残留在水环境中,威胁水生动物的生存和繁衍。       

The Presence and Localization of Melatonin Receptors in the Rat Aorta

Melatonin is involved in blood pressure modulation in rats and humans. Some of the effects of melatonin are presumably mediated via two G-protein-coupled receptors (MT₁ and MT₂), but the distribution of MT₁ and MT₂ in the cardiovascular system remains to be explored comprehensively. We investigated the expression of both the receptors in the rat aorta on mRNA level by RT-PCR and real time RT-PCR as well as on protein level via western blotting and immunofluorescence microscopy. We verified MT₁ mRNA expression in the rat aorta and demonstrated the absence of MT₂ mRNA in this vessel type. MT₁ receptors were confirmed also at the protein level, and surprisingly they were preferentially localized to the tunica adventitia. Since no daily changes in MT₁ mRNA expression were detected, we suppose that the circadian changes in circulating melatonin concentrations are sufficient to mediate circadian effects of melatonin in the aorta. The localization of MT₁ in the tunica adventitia suggests an influence of melatonin on vasa vasorum function and signal transduction in the aorta wall.     

马来酸氟伏沙明片联合氨磺必利治疗精神分裂症患者的疗效及安全性分析

目的:分析马来酸氟伏沙明片联合氨磺必利治疗精神分裂症患者的临床疗效及安全性。方法:选择我院2014年2月~2018年2月收治的182例精神分裂症患者,按随机数字表法分为对照组99例和研究组83例。对照组采用氨磺必利治疗,研究组在对照组基础上联合马来酸氟伏沙明片治疗。比较两组临床疗效,治疗前后血脂代谢、总胆汁酸(TBA)水平,阳性与阴性症状量表(PANSS)评分,生活质量,及不良发生情况。结果:治疗后,研究组总有效率为91.57%,显著高于对照组(P<0.05);两组治疗前后空腹血糖(FPG)、糖化血红蛋白(HbA1c)、甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白-C(LDL-C)、高密度脂蛋白-C(HLD-C)、总胆汁酸(TBA)水平比较均无统计学意义(P>0.05);两组治疗后PANSS评分均较治疗前显著下降,生活质量评分较治疗前均明显上升,且研究组PANSS评分显著低于对照组,而生活质量评分明显高于对照组,差异均有统计学意义(P<0.05)。治疗过程中,两组均有体重增加、口渴及便秘发生,两组不良反应发生情况比较差异无统计学意义(P>0.05)。结论:马来酸氟伏沙明片联合氨磺必利治疗能够提高精神分裂症患者疗效,对机体糖脂代谢及肝功能影响较小,有良好的用药安全性。     

水稻原生质体制备条件的优化及其细胞壁变化的快速检测

以疣粒野生稻和栽培稻02428的成熟种子为材料,对愈伤组织的诱导和继代、胚性悬浮细胞系建立、原生质体制备、再生细胞团分化及植株再生进行研究。结果表明：（1）水稻愈伤组织诱导的最佳2,4-D浓度为0.014 mmol/L;（2）胚性悬浮细胞系建立的最佳条件为AA 悬浮培养基+ 0.009 mmol/L 2,4-D ,每25 mL液体培养基加入0.4 g愈伤组织的初始接种量,7 d的继代周期;（3）原生质体制备的最佳条件为20 g/L纤维素酶+ 1 g/L果胶酶,酶解5 h,800 r/min离心5 min;（4）用荧光增白剂（VBL）细胞壁染色液可以快速、准确的检测原生质体制备及培养过程中细胞壁的变化情况。     

两株四环素降解菌的分离鉴定及降解条件优化

为发掘四环素高效降解菌株,本研究从以四环素为唯一生长碳源的养鸡场粪便堆肥样品中分离筛选到2株四环素降解菌TC-04和TC-09,并通过形态特征、生理生化特征和16SrRNA基因序列分析,对其进行鉴定.采用单因素试验分别探究不同碳源、氮源、微量元素这3个培养基成分和培养时间、接种量和装液量这3个培养条件对两株菌降解四环素...     

中国肩花蝽属雌性交配管的形态研究

半翅目花蝽科（Anthocoridae） 昆虫的种类鉴定通常是以雄性外生殖器特征为主, 雌虫的种类鉴定一直是研究中的难点。本文研究了中国肩花蝽属 Tetraphleps 已知5个种雌虫的交配管,包括黑色肩花蝽T. aterrimus (Sahlberg)、斑翅肩花蝽T. galchanoides Ghauri、毛肩花蝽T. pilosulus Bu et Zheng、直长肩花蝽T. parallelus Bu et Zheng和玉龙肩花蝽T. yulongensis Bu et Zheng。发现交配管的构造在种内变异小、特征稳定,而在种间差别明显,可作为该属雌虫种类鉴定的可靠依据。     

野生与笼养绿孔雀种群的随机扩增多态DNA研究

利用随机扩增多态DNA（RAPD）技术对野生14只和笼养18只绿孔雀（Pavo muticus）个体进行了种群遗传多样性分析。用23个随机引物,野生与笼养绿孔雀分别获得161和166个扩增片段,计算发现野生与笼养绿孔雀的种群内平均相对遗传距离分别是0.0555和0.1355,两种群间的为0.1635;两种群的Shannon多样性指数平均分别是0.4348和1.0163,有显著性差异。以上分析都显示野生绿孔雀的遗传多样性很低。用UPGMA法聚类显示两个种群都是分别来源于两个家系,可据此进行繁育管理。 Abstract:Random-amplified polymorphic DNA(RAPD) was used to investigate the genetic diversity of the population of 14 wild green peafowl and 18 captive green peafowl(pavo muticus).Total of 161 and 166 bands were obtained respectively,and 23 random primers were used to amplify the genomic DNA of the wild and captive green peafowls.The average relative hereditary distance of the wild and captive green peafowls is 0.0555 and 0.1355 respectively;and the Shannon diversity index is 0.4348 and 1.0163 respectively.There is a prominent differentia between the two populations by T-Test of HO.All the analyses above show that the genetic diversity is very low in wild green peafowl.It tells us that the two populations come from two families by using UPGMA,which can be useful in the breeding management in the future.     

安徽清凉峰自然保护区大型真菌的生态分布

清凉峰北坡的大型真菌十分丰富,经初步调查,发现清凉峰北坡共有大型真菌2纲10目38科72属160种,它们广泛分布于常绿阔叶林、马尾松林、常绿-落叶混交林、落叶阔叶林和黄山松林等林带中。按经济价值分有食用菌72种,药用菌55种,毒菌19种;依生态习性分有木生菌68种,土生菌84种,菌根真菌21种。本文论述了该地区大型真菌自然发生的常见种类和分布,并对该区大型真菌资源进行了评价,大型真菌在各林带下的分布有一定规律性,种群的组成和分布与植被类型、林中小生境、土壤类型、海拔高度等相关。该区有多种具有经济价值的大型真菌,是主要的自然资源之一,它们在食用、药用、营林等方面有着较大的利用潜力。