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941.
Estrogen inhibits osteoclasts formation and bone resorption via microRNA-27a targeting PPARγ and APC
Lei Guo Kaizhe Chen Jun Yuan Ping Huang Xing Xu Changwei Li Niandong Qian Jin Qi Zhiliang Shao Lianfu Deng Chuan He Jiping Xu 《Journal of cellular physiology》2019,234(1):581-594
Inhibition of osteoclasts formation and bone resorption by estrogen is very important in the etiology of postmenopausal osteoporosis. The mechanisms of this process are still not fully understood. Recent studies implicated an important role of microRNAs in estrogen-mediated responses in various cellular processes, including cell differentiation and proliferation. Thus, we hypothesized that these regulatory molecules might be implicated in the process of estrogen-decreased osteoclasts formation and bone resorption. Western blot, quantitative real-time polymerase chain reaction, tartrate-resistant acid phosphatase staining, pit formation assay and luciferase assay were used to investigate the role of microRNAs in estrogen-inhibited osteoclast differentiation and bone resorption. We found that estrogen could directly suppress receptor activator of nuclear factor B ligand/macrophage colony-stimulating factor-induced differentiation of bone marrow-derived macrophages into osteoclasts in the absence of stromal cell. MicroRNA-27a was significantly increased during the process of estrogen-decreased osteoclast differentiation. Overexpressing of microRNA-27a remarkably enhanced the inhibitory effect of estrogen on osteoclast differentiation and bone resorption, whereas which were alleviated by microRNA-27a depletion. Mechanistic studies showed that microRNA-27a inhibited peroxisome proliferator-activated receptor gamma (PPARγ) and adenomatous polyposis coli (APC) expression in osteoclasts through a microRNA-27a binding site within the 3′-untranslational region of PPARγ and APC. PPARγ and APC respectively contributed to microRNA-27a-decreased osteoclast differentiation and bone resorption. Taken together, these results showed that microRNA-27a may play a significant role in the process of estrogen-inhibited osteoclast differentiation and function. 相似文献
942.
Qiang‐Qiang Xiong Tian‐Hua Shen Lei Zhong Chang‐Lan Zhu Xiao‐Song Peng Xiao‐Peng He Jun‐Ru Fu Lin‐Juan Ouyang Jian‐Min Bian Li‐Fang Hu Xiao‐Tang Sun Jie Xu Hui‐Ying Zhou Hao‐Hua He Xiao‐Rong Chen 《Physiologia plantarum》2019,167(4):564-584
Abrupt drought–flood alternation (T1) is a meteorological disaster that frequently occurs during summer in southern China and the Yangtze river basin, often causing a significant loss of rice production. In this study, the response mechanism of yield decline under abrupt drought–flood alternation stress at the panicle differentiation stage was analyzed by looking at the metabolome, proteome as well as yield and physiological and biochemical indexes. The results showed that drought and flood stress caused a decrease in the yield of rice at the panicle differentiation stage, and abrupt drought–flood alternation stress created a synergistic effect for the reduction of yield. The main reason for the decrease of yield per plant under abrupt drought–flood alternation was the decrease of seed setting rate. Compared with CK0 (no drought and no flood), the net photosynthetic rate and soluble sugar content of T1 decreased significantly and its hydrogen peroxidase, superoxide dismutase, peroxidase activity increased significantly. The identified differential metabolites and differentially expressed proteins indicated that photosynthesis metabolism, energy metabolism pathway and reactive oxygen species response have changed strongly under abrupt drought–flood alteration stress, which are factors that leads to the rice grain yield reduction. 相似文献
943.
Plant and Soil - Cadmium (Cd) is the most widespread toxic heavy metal to plant growth. As the second leading oil crop, some genotypes of Brassica napus (B. napus) are potential Cd accumulators.... 相似文献
944.
Plant and Soil - Current knowledge of soil heterogeneity-diversity relationships (HDR) is largely based on studies manipulating single factor, but the advancements in HDR may require a... 相似文献
945.
946.
Han Feng Liu Liping Lu Jiaxi Chai Yingjuan Zhang Jie Wang Shijin Sun Licui Wang Qin Liu Yiqun He Mengjie Mu Weipeng Huang Zhenwu 《Biological trace element research》2019,188(2):363-372
Biological Trace Element Research - The required selenium intake for optimal health in Chinese residents was published in 2014. However, the adequate intake (AI) value for Chinese infants... 相似文献
947.
He Pei Wu Long-Fei Bing Peng-Fei Xia Wei Wang Lan Xie Fang-Fei Lu Xin Lei Shu-Feng Deng Fei-Yan 《Molecular and cellular biochemistry》2019,450(1-2):135-147
Molecular and Cellular Biochemistry - The immune responses, involved in recognition of cancer-specific antigens, are of particular interest as this may provide major leads towards developing new... 相似文献
948.
Molecular Biology Reports - The multidrug and toxic compound extrusion (MATE) protein family is a newly discovered family of secondary transporters that extrude metabolic waste and a variety of... 相似文献
949.
He Youdi Chen Jun-Feng Yang Yan-Mei Huang Xiao-Hui Dong Xiao-Hui Yang Hui-Xin Cao Jun-Kai Jiang Xiao-Xia 《Molecular biology reports》2019,46(4):3991-3999
Molecular Biology Reports - Mesenchymal stem cells (MSCs) are self-renewing multipotent cells with immunoregulatory function, which makes them attractive candidates for regenerative medicine.... 相似文献
950.