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Steve Harakeh Esam Azhar Saad Almasaudi Kohar Berge Kissoyan Sukayna Fadlallah Arax Tanelian Ghassan M. Matar 《Saudi Journal of Biological Sciences》2019,26(7):1576-1580
Background and aimExtended-spectrum beta-lactamases are the main cause of resistance in Enterobacteriaceae to beta lactam antibiotics. The aim of this study was to evaluate the antimicrobial effect of EpiQuercican supplement, combined with different antimicrobial agents, on ESBL-producing isolates and determine the underlying molecular mechanism of resistance in these isolates.Materials and methodsEleven ESBL producing Enterobacteriaceae isolates were collected from Saudi Arabia hospitals between 2016 and 2017 and disk diffusion test was performed in accordance with Clinical and Laboratory Standards Institute (CLSI) guidelines to determine the susceptibility of the isolates to 5 different antibiotics in the presence of EpiQuercican supplement. Polymerase chain reaction was performed for detection of ESBL genes, and efflux pump inhibitor was used to study the mechanism of resistance in these isolates.ResultsThe best synergistic effect was obtained when the supplement was combined with carbapenems followed by 4th generation cephalosporins. Either no effect or antagonistic effect was seen with most of the isolates when the supplement was added to the 3rd generation of cephalosporins. Among the tested genes responsible for ESBL production in this study, our results indicated the predominance of TEM genes (73%) followed by CTX-M genes (9%). As for the mechanism of resistance in ESBL isolates, 4 isolates showed to use efflux pumps as their main mechanism of resistance.ConclusionThe EpiQuercican supplement showed some promising results, yet its antibacterial mechanism of action needs to be elucidated further. 相似文献
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Random amplified polymorphic DNA (RAPD) analysis was done on 32 isolates of Pseudomonas aeruginosa. These isolates were obtained from 22 patients who presented to the emergency room in a major medical center in Beirut, Lebanon,
during a 5-month period with the diagnosis of either unilateral or bilateral otitis externa. Patients had yellowish to greenish
discharge, moderate to severe external auditory canal swelling, moderate to severe pain, and periauricular cellulitis. None
of these patients had intrinsic predisposing factors. An ear swab was obtained from both ears of patients, cultured on trypticase
soy agar. P. aeruginosa was identified on the basis of pyocyanine production and API identification kits. RAPD analysis was done by using two primers
(10 mer and 21 mer primers) and appropriate PCR conditions on extracted DNA. Our data have shown 23 RAPD patterns (A–W) distributed
among the 32 P. aeruginosa isolates. RAPD patterns were reproducible. Twenty of 32 isolates were recovered from 10 patients with bilateral otitis externa.
The remaining 12 of 32 isolates were recovered from 12 different patients with unilateral otitis externa. Eleven RAPD patterns
(A,B,C,D,E,F,H,I,R,U,V) were associated with severe clinical symptoms, including severe pain, severe external auditory canal
swelling, periauricular cellulitis, and a yellowish discharge. The remaining RAPD patterns were not associated with severe
infections. This denotes a possible association between certain genotypes and severity of symptoms.
Received: 6 July 2000 / Accepted: 5 September 2000 相似文献
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M.S. Harakeh 《FEMS microbiology letters》1984,23(1):21-26
Abstract The effects of the combination of any two of the following disinfectants: chlorine, chlorine dioxide, ozone and peracetic acid were investigated using poliovirus 1 as a model virus in a municipal sewage effluent. It was noted that the efficacy of chlorine was enhanced in the presence of either chlorine dioxide, ozone or peracetic acid. Maximum enhancement was achieved when peracetic acid was present with either chlorine or ozone and less enhancement was noted when the peracetic acid was added before chlorine dioxide. Similar results were noted when hydrochloric acid was used instead of peracetic acid. It may be concluded that the application of any two of the disinfectants studied were rarely synergistic but merely additive or complementary (i.e., one disinfectant provides the other with a medium in which it functions better). 相似文献
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Susceptibility of chemostat-grown Yersinia enterocolitica and Klebsiella pneumoniae to chlorine dioxide. 总被引:5,自引:5,他引:0 下载免费PDF全文
The resistance of bacteria to antimicrobial agents could be influenced by growth environment. The susceptibility of two enteric bacteria, Yersinia enterocolitica and Klebsiella pneumoniae, to chlorine dioxide was investigated. These organisms were grown in a defined medium in a chemostat and the influence of growth rate, temperature, and cell density on the susceptibility was studied. All inactivation experiments were conducted with a dose of 0.25 mg of chlorine dioxide per liter in phosphate-buffered saline at pH 7.0 and 23 degrees C. The results indicated that populations grown under conditions that more closely approximate natural aquatic environments, e.g., low temperatures and growth at submaximal rates caused by nutrient limitation, were most resistant. The conclusion from this study is that antecedent growth conditions have a profound effect on the susceptibility of bacteria to disinfectants, and it is more appropriate to use the chemostat-grown bacteria as test organisms to evaluate the efficacy of a certain disinfectant. 相似文献
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Maya L. Gosztyla Lydia Kwong Naomi A. Murray Claire E. Williams Nicholas Behnke Porsia Curry Kevin D. Corbett Karen N. DSouza Julia Gala de Pablo Joanina Gicobi Monica Javidnia Navina Lotay Sidney Madison Prescott James P. Quinn Zeena M. G. Rivera Markia A. Smith Karen T. Y. Tang Aarya Venkat Megan A. Yamoah 《PLoS computational biology》2021,17(7)
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M.S. Harakeh 《FEMS microbiology letters》1984,23(1):27-30
Abstract Cells of B. subtilis are able to synthesize cAMP in response to oxygen limitation. Several methods were used to characterize cAMP in cell extracts. Using [3 H]adenosine, cAMP synthesis could also be detected under in vivo conditions. Furthermore, activities of phosphodiesterase and adenylate cyclase were measured in extracts of B. subtilis cells and in membrane fractions. We suggest that in B. subtilis cAMP is synthesized only under special physiological conditions. 相似文献
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Sarai R. Boelema Zeena Harakeh Martine J. E. van Zandvoort Sijmen A. Reijneveld Frank C. Verhulst Johan Ormel Wilma A. M. Vollebergh 《PloS one》2015,10(10)