Dominating species of entomophilous ascomycetes anamorphs in West Siberia,Primorsky krai,and Kyrgyzstan

The paper studies mycobiota of the dead insects in West Siberia, Primorsky krai, and Kyrgyzstan. Ascomycetes anamorphs of 13 genera are revealed. In all regions Beauveria bassiana (Bals.-Criv.) Vuill. dominated comprising on average 68% of the total number of isolates. The fungus hosts list the insects of 7 orders and 32 families with Coleoptera, Lepidoptera, and Hemiptera dominating. The rarely found entomopathogens include Tolypocladium inflatum Gams (primarily on Lepidoptera), Metarhizium anisopliae (Metschn.) Sorokin (on Coleoptera). The mortality rate of the insects due to micromycetes is observed mainly on enzootic level. The study of the pathogenic properties of the dominating species (B. bassiana) show the absence of specificity of its environmental isolates for a number of representatives of Orthoptera, Lepidoptera, Coleoptera, and Diptera.     

Theoretical and experimental studies of the radiative properties of matter at high energy densities and their application to the problems of inertial confinement fusion

The paper presents the results of theoretical and experimental studies of the radiative properties of plasmas produced by heating and compression of various materials to high energy densities. The specific features of the theoretical plasma model known as the ion model, which is used to calculate the radiative characteristics of plasmas of complex chemical composition, are discussed. The theoretical approach based on this model is applied to the plasma produced during the explosion of the X-pinch wires. The theoretical estimate of the radiation efficiency is compared with the experimental data on the total energy yield from an X-pinch made of two different wires (NiCr and Alloy 188). The radiative characteristics of (C12 H16 O8) and (C8 H12 O6) plasmas are calculated for the temperature diagnostics of plasmas produced from porous targets employed in inertial confinement fusion experiments with the use of laser radiation and heavy-ion beams.     

A rapid method for the purification of supercoiled PM2 DNA by affinity chromatography on H1 histone covalently coupled to agarose.

A simple and rapid method is described for the purification of supercoiled PM2 DNA by affinity chromatography on columns of H1 histone covalently coupled to agarose. The method does not require the use of intercalating agents or ultracentrifugation procedures. Under the conditions most appropriate for purification, elution is carried out in a single step with buffered 0.7 M NaCl after the sample has been loaded onto the column in buffered 0.2 M NaCl. The DNA eluted at the higher salt concentration consists of supercoiled closed circular DNA at greater than 90% purity independently of the ratio of supercoiled to nicked circular DNA in the input mixture.