全文获取类型
收费全文 | 363篇 |
免费 | 7篇 |
出版年
2023年 | 3篇 |
2018年 | 7篇 |
2017年 | 6篇 |
2016年 | 7篇 |
2015年 | 11篇 |
2014年 | 16篇 |
2013年 | 27篇 |
2012年 | 23篇 |
2011年 | 15篇 |
2010年 | 27篇 |
2009年 | 15篇 |
2008年 | 4篇 |
2007年 | 5篇 |
2006年 | 6篇 |
2005年 | 5篇 |
2004年 | 6篇 |
2003年 | 6篇 |
2001年 | 5篇 |
1998年 | 3篇 |
1997年 | 5篇 |
1996年 | 3篇 |
1994年 | 4篇 |
1993年 | 4篇 |
1991年 | 5篇 |
1988年 | 6篇 |
1986年 | 3篇 |
1985年 | 5篇 |
1984年 | 4篇 |
1983年 | 7篇 |
1982年 | 3篇 |
1981年 | 5篇 |
1979年 | 10篇 |
1978年 | 6篇 |
1977年 | 5篇 |
1976年 | 5篇 |
1975年 | 4篇 |
1974年 | 4篇 |
1973年 | 7篇 |
1972年 | 3篇 |
1971年 | 3篇 |
1967年 | 3篇 |
1966年 | 5篇 |
1965年 | 5篇 |
1964年 | 4篇 |
1962年 | 3篇 |
1961年 | 2篇 |
1959年 | 5篇 |
1958年 | 12篇 |
1957年 | 5篇 |
1956年 | 4篇 |
排序方式: 共有370条查询结果,搜索用时 15 毫秒
131.
Jiří Wiesner Zdeněk Kříž Kamil Kuča Daniel Jun Jaroslav Koča 《Journal of enzyme inhibition and medicinal chemistry》2013,28(4):417-424
Acetylcholinesterase (AChE) is a widely spread enzyme playing a very important role in nerve signal transmission. As AChE controls key processes, its inhibition leads to the very fast death of an organism, including humans. However, when this feature is to be used for killing of unwanted organisms (i.e. mosquitoes), one is faced with the question - how much do AChEs differ between species and what are the differences? Here, a theoretical point of view was utilized to identify the structural basis for such differences. The various primary and tertiary alignments show that AChEs are very evolutionary conserved enzymes and this fact could lead to difficulties, for example, in the search for inhibitors specific for a particular species. 相似文献
132.
Three different methods were used for the monitoring of airborne microorganisms: (1). Cultivation of microbes trapped in a single-stage biological impactor directly on a solid agar nutrient medium (meat-pepton agar, Sabouraud's agar, blood agar) in Petri dishes. The repeated yearly course of concentrations of cultivable organisms, or colony-forming units (CFU), was obtained by long-run measurements. (2) Aeresol was trapped by impact on membrane filters, and the microorganisms were cultivated by placing the filters on the agar media as above. (3) Direct microorganism counting in a fluorescence microscope; air was sampled in a four-stage impactor where the aerosol was trapped on microscope slides, and the microorganisms were subsequently stained with fluorescent dyes (fluorescein diacctate, 4;6-diamidino-2-phenylindole and, particular, ethidium bromide). The highest microorganism counts were obtained by using the fluorescence method, the direct cultivation method gave counts an order of magnitude lower, and the method of cultivation on filters gave values approximately 10 times lower than the conventional cultivation. High variations in the airborne CFU concentrations over the year were observed in Prague. Over the winter season the variations in the amounts of airborne bacteria and other micromycetes as well as the amounts themselves were lower than in the remaining seasons. In the spring and in the summer, the concentrations of yeasts and other micromycetes were highest, whereas in the autumn the concentrations of the microorganisms decreased. Among the bacteria cultivated form the airborne aerosol, the genera Micrococcus, Bacillus, Neisseria and Corynebacterium predominated. The prevailing genera of micromycetes were Penicillium, Aspergillus and Cladosporium. The concentrations of microorganisms in free air were also affected by the local weather conditions, temperature in particular, the overall air pollution by aerosols was of minor importance in this respect. 相似文献
133.
134.
We investigated the interaction between two invasive invertebrate species in a shallow Central European flooded sandpit: the
epibiosis of Ponto-Caspian zebra mussels Dreissena polymorpha on the American crayfish Orconectes limosus. Between 2004 and 2005, we followed the seasonal variation in number and size of the mussels attached to crayfish bodies,
and microhabitats preferred by mussels. The proportion of crayfish colonised by mussels varied seasonally: in spring and early
summer it was consistently over 75%, afterwards it dropped temporarily due to loss of bivalves during the crayfish moult,
and later increased again due to re-colonisation by often relatively large juvenile mussels. Three different pathways of mussel
settlement on crayfish hosts are likely: (1) primary settlement of free-swimming pediveliger larvae; (2) secondary settlement
of plantigrade mussels and juveniles; (3) active re-attachment of grown mussels from the substrate to crayfish. This epibiosis
was promoted by lack of suitable substrates at the studied locality.
Electronic supplementary material The online version of this article (doi: ) contains supplementary material, which is available to authorized users. 相似文献
135.
Alena Fornůsková Eric J. Petit Tomáš Bartonička Peter Kaňuch Alain Butet Zdeněk Řehák Josef Bryja 《Biological journal of the Linnean Society. Linnean Society of London》2014,113(4):1115-1125
The ontogeny and heritability of echolocation, an important sense in echolocating bats, is still not completely understood. Intraspecific variation in echolocation calls can be high, although the importance of possible explanatory variables (e.g. age, sex, social groups) remains largely unknown. Echolocation pulse features may vary among maternity roosts and this can theoretically be caused either by intercolony genetic differences or by vocal dialects learned during ontogeny within a roost (or a combination of both). In the present study, we analyzed intraspecific variation in echolocation parameters in relation to genetic structure at bi‐parentally inherited microsatellites and maternally inherited mitochondrial (mt)DNA in maternal colonies of Pipistrellus pipistrellus in Central Europe. We found that individual colonies differ significantly in mtDNA, whereas the structure on nuclear markers is almost absent. This suggests a typical temperate bat social structure pattern, with strong sex‐biased dispersal (i.e. philopatric females and dispersing males) (up to 92% of males leave their birth place according to our results). However, we show for the first time that genetic differentiation among mtDNA matrilines is associated with significant intercolony echolocation parameter differences. Because the genetic component of echolocation is not likely to be encoded by mtDNA, the results support the hypothesis of maternal echolocation dialect transmission to offspring, and the role of learning in this process is discussed. © 2014 The Linnean Society of London, Biological Journal of the Linnean Society, 2014, 113 , 1115–1125. 相似文献
136.
The effect of different growing substrates on the development of the slug parasite Phasmarhabditis hermaphrodita has been studied in a series of laboratory experiments. Wild, laboratory and Nemaslug strains of P. hermaphrodita and their monoxenic counterparts with Moraxella osloensis (CCM 5605T) were reared on homogenised pig kidney, Deroceras reticulatum, Arion lusitanicus, and Galleria mellonella, the faeces of D. reticulatum and A. lusitanicus, or leaf compost. Development time, yield, lipid reserves and the body length of nematodes were assessed. All P. hermaphrodita strains were able to grow and reproduce on all tested substrates; however, yields were markedly higher on invertebrate media. Lipid content and body size varied across the substrates and strains. A uniform response of all nematode strains and the most dramatic differences among substrates were recorded in yields, which could indicate that the quality of the substrate is expressed mainly in yield. Monoxenized strains had higher yield, length, lipid content and shorter development time. In general, the strain differences between the observed parameters were most likely due to different bacterial associates. The dramatic differences in yields on invertebrate substrates, in comparison to the others, illustrate the evolutionary advantage of the association of nematodes with invertebrates. 相似文献
137.
Houštek J Hejzlarová K Vrbacký M Drahota Z Landa V Zídek V Mlejnek P Šimáková M Šilhavy J Mikšík I Kazdová L Oliyarnyk O Kurtz T Pravenec M 《Physiological genomics》2012,44(9):487-494
Common inbred strains of the laboratory rat can be divided into four different mitochondrial DNA haplotype groups represented by the SHR, BN, LEW, and F344 strains. In the current study, we investigated the metabolic and hemodynamic effects of the SHR vs. LEW mitochondrial genomes by comparing the SHR to a new SHR conplastic strain, SHR-mt(LEW); these strains are genetically identical except for their mitochondrial genomes. Complete mitochondrial DNA (mtDNA) sequence analysis comparing the SHR and LEW strains revealed gene variants encoding amino acid substitutions limited to a single mitochondrial enzyme complex, NADH dehydrogenase (complex I), affecting subunits 2, 4, and 5. Two of the variants in the mt-Nd4 subunit gene are located close to variants known to be associated with exercise intolerance and diabetes mellitus in humans. No variants were found in tRNA or rRNA genes. These variants in mt-Nd2, mt-Nd4, and mt-Nd5 in the SHR-mt(LEW) conplastic strain were linked to reductions in oxidative and nonoxidative glucose metabolism in skeletal muscle. In addition, SHR-mt(LEW) conplastic rats showed increased serum nonesterified fatty acid levels and resistance to insulin stimulated incorporation of glucose into adipose tissue lipids. These results provide evidence that inherited variation in mitochondrial genes encoding respiratory chain complex I subunits, in the absence of variation in the nuclear genome and other confounding factors, can influence glucose and lipid metabolism when expressed on the nuclear genetic background of the SHR strain. 相似文献
138.
Claus Orendt Georg Wolfram Zdeněk Adámek Pavel Jurajda Mechthild Schmitt-Jansen 《Biologia》2012,67(1):180-199
Macroinvertebrate communities were investigated along a gradient of heavy industrial and municipal pollution in the highland
Bílina River (Czech Republic). Physico-chemical determinants and ions were monitored and community analysis performed focusing
on taxonomic composition, ecological functioning (feeder and dweller guilds) and water quality metrics, including saprobity
index, BMWP and diversity. Impacted sites differed significantly from reference and from recovered stretches. Chemical data
revealed two main pollution factors, (1) a “salinity determinant”, described best by conductivity and SO42−, and (2) an “organic pollution determinant”, represented best by O2 concentrations and NO2−, all varying locally and temporally. Some metrics and taxa showed significant correlations to abiotic parameters. Functional
communities showed a stronger relationship to the “organic pollution determinant”, suggesting that elevated organic pollution
had a dominating influence on functional community metrics; though other variables may also have an influence in this multistress
environment. On the other hand, there were indications that the taxonomic community was more influenced by ion concentrations
(“salinity determinant”). The gradient from reference sites to polluted sites was weaker in the final sampling campaign. The
results presented here can be used as a reference for assessing future changes in environmental impact from pollution, being
finer and more detailed than assessment according to the EU’s WFD. 相似文献
139.
Drahota Z Endlicher R Staňková P Rychtrmoc D Milerová M Cervinková Z 《Journal of bioenergetics and biomembranes》2012,44(3):309-315
We describe a new method for the analysis of mitochondrial swelling curves. Using classical swelling curves, only the maximum extent of the swelling can be calculated in a numerical form. However, taking the derivative of the classical swelling curves enables the evaluation of two additional parameters of the swelling process in a numerical form, namely, the maximum swelling rate after the addition of the swelling inducer (as dA???/10 s) and the time (in sec) at which the maximum swelling rate after the addition of the swelling inducer is obtained. The use of these three parameters enables the better characterization of the swelling process as demonstrated by the evaluation of calcium and phosphate interactions in the opening of the mitochondrial permeability transition pore and by the characterization of the peroxide potentiating action. 相似文献
140.
Levin BA Freyhof J Lajbner Z Perea S Abdoli A Gaffaroğlu M Ozuluğ M Rubenyan HR Salnikov VB Doadrio I 《Molecular phylogenetics and evolution》2012,62(1):542-549
We reconstructed the matrilineal phylogeny of Asian algae-eating fishes of the genus Capoeta based on complete mitochondrial gene for cytochrome b sequences obtained from 20 species sampled from the majority of the range and 44 species of closely related barbs of the genera Barbus s. str. and Luciobarbus. The results of this study show that Capoeta forms a strongly supported monophyletic subclade nested within the Luciobarbus clade, suggesting that specialized scraping morphology appeared once in the evolutionary history of the genus. We detected three main groups of Capoeta: the Mesopotamian group, which includes three species from the Tigris-Euphrates system and adjacent water bodies, the Anatolian-Iranian group, which has the most diversified structure and encompasses many species distributed throughout Anatolian and Iranian inland waters, and the Aralo-Caspian group, which consists of species distributed in basins of the Caspian and Aral Seas, including many dead-end rivers in Central Asia and Northern Iran. The most probable origination pathway of the genus Capoeta is hypothesized to occur as a result of allopolyploidization. The origin of Capoeta was found around the Langhian-Serravallian boundary according to our molecular clock. The diversification within the genus occurred along Middle Miocene-Late Pliocene periods. 相似文献