首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   9697篇
  免费   449篇
  国内免费   1篇
  2021年   105篇
  2019年   98篇
  2018年   218篇
  2017年   190篇
  2016年   246篇
  2015年   259篇
  2014年   294篇
  2013年   399篇
  2012年   438篇
  2011年   449篇
  2010年   329篇
  2009年   287篇
  2008年   346篇
  2007年   315篇
  2006年   326篇
  2005年   268篇
  2004年   297篇
  2003年   260篇
  2002年   218篇
  2001年   218篇
  2000年   198篇
  1999年   171篇
  1998年   114篇
  1992年   129篇
  1991年   110篇
  1990年   141篇
  1989年   113篇
  1988年   119篇
  1987年   109篇
  1986年   111篇
  1985年   113篇
  1984年   129篇
  1983年   101篇
  1982年   96篇
  1981年   102篇
  1980年   93篇
  1979年   133篇
  1978年   107篇
  1977年   118篇
  1976年   95篇
  1975年   128篇
  1974年   132篇
  1973年   132篇
  1972年   128篇
  1971年   93篇
  1970年   120篇
  1969年   132篇
  1968年   100篇
  1967年   104篇
  1966年   110篇
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
Genetic substructuring in plant populations may evolve as a consequence of sampling events that occur when the population is founded or regenerated, or if gene dispersal by pollen and seeds is restricted within a population. Silene tatarica is an endangered, perennial plant species growing along periodically disturbed riverbanks in northern Finland. We investigated the mechanism behind the microspatial genetic structure of S. tatarica in four subpopulations using amplified fragment length polymorphism markers. Spatial autocorrelation revealed clear spatial genetic structure in each subpopulation, even though the pattern diminished in older subpopulations. Parentage analysis in an isolated island subpopulation indicated a very low level of selfing and avoidance of breeding between close relatives. The mean estimated pollen dispersal distance (24.10 m; SD = 10.5) was significantly longer and the mean seed dispersal distance (9.07 m; SD = 9.23) was considerably shorter than the mean distance between the individuals (19.20 m; SD = 13.80). The estimated indirect and direct estimates of neighbourhood sizes in this subpopulation were very similar, 32.1 and 37.6, respectively. Our results suggested that the local spatial genetic structure in S. tatarica was attributed merely to the isolation-by-distance process rather than founder effect, and despite free pollen movement across population, restricted seed dispersal maintains local genetic structure in this species.  相似文献   
992.
Helminth genome projects: all or nothing   总被引:1,自引:0,他引:1  
  相似文献   
993.
994.
Коммуникации занимается дегенерации производства штамма Streptomyces erythreus. Новые Jare представлены выводы:
  1. (1)
    Штамма включает в себя как sporogenous и asporogenous морфологических компонент, каждая из которых может быть делится на подтипы далее. По оценке производственной деятельности индивидуального морфологические подтипов было показано что sporogenous компонент культуры включает в себя лиц, в то время производственного asporogenous компонент включает в себя только непроизводственной или с низким уровнем производственного лиц. Микроскопического изучения показали, что sporogenous подтипов формируется прямо вегетативной hyphae, в то время как asporogenous подтипов формируется толще и сморщенное вегетативной hyphae.  相似文献   
995.
996.
BBK32 is a fibronectin-binding protein from the Lyme disease-causing spirochete Borrelia burgdorferi. In this study, we show that BBK32 shares sequence similarity with fibronectin module-binding motifs previously identified in proteins from Streptococcus pyogenes and Staphylococcus aureus. Nuclear magnetic resonance spectroscopy and isothermal titration calorimetry are used to confirm the binding sites of BBK32 peptides within the N-terminal domain of fibronectin and to measure the affinities of the interactions. Comparison of chemical shift perturbations in fibronectin F1 modules on binding of peptides from BBK32, FnBPA from S. aureus, and SfbI from S. pyogenes provides further evidence for a shared mechanism of binding. Despite the different locations of the bacterial attachment sites in BBK32 compared with SfbI from S. pyogenes and FnBPA from S. aureus, an antiparallel orientation is observed for binding of the N-terminal domain of fibronectin to each of the pathogens. Thus, these phylogenetically and morphologically distinct bacterial pathogens have similar mechanisms for binding to human fibronectin.  相似文献   
997.
The effect of noncatalytic domains 2+3 on the intrinsic activity and thermostability of the EF-Tu GTPase center was evaluated in experiments with isolated domains 1 and six chimeric variants of mesophilic Escherichia coli (Ec) and thermophilic Bacillus stearothermophilus (Bst) EF-Tus. The isolated catalytic domains 1 of both EF-Tus displayed similar GTPase activities at their optimal temperatures. However, noncatalytic domains 2+3 of the EF-Tus influenced the GTPase activity of domains 1 differently, depending on the domain origin. Ecdomains 2+3 suppressed the GTPase activity of the Ecdomain 1, whereas those of BstEF-Tu stimulated the Bstdomain 1 GTPase. Domain 1 and domains 2+3 of both EF-Tus positively cooperated to heat-stabilize their GTPase centers to attain optimal activity at a temperature close to the optimal growth temperature of either organism. This can be explained by a stabilization effect of domains 2+3 on alpha-helical regions of the G-domain as revealed by CD spectroscopy.  相似文献   
998.
999.
With reference to two specific modalities of sensation, the taste of saltiness of chloride salts, and the loudness of steady tones, it is shown that the laws of sensation (logarithmic and power laws) are expressions of the entropy per mole of the stimulus. That is, the laws of sensation are linear functions of molar entropy. In partial verification of this hypothesis, we are able to derive an approximate value for the gas constant, a fundamental physical constant, directly from psychophysical measurements. The significance of our observation lies in the linking of the phenomenon of “sensation” directly to a physical measure. It suggests that if the laws of physics are universal, the laws of sensation and perception are similarly universal. It also connects the sensation of a simple, steady physical signal with the molecular structure of the signal: the greater the number of microstates or complexions of the stimulus signal, the greater the magnitude of the sensation (saltiness or loudness). The hypothesis is currently tested on two sensory modalities.  相似文献   
1000.
Zupán K  Herényi L  Tóth K  Egyeki M  Csík G 《Biochemistry》2005,44(45):15000-15006
We studied the complex formation of tetrakis(4-N-methylpyridyl)porphyrin (TMPyP) with double stranded DNAs and T7 phage nucleoprotein complex. We analyzed the effect of base pair composition of DNA, the presence of capsid protein, and the composition of the microenvironment on the distribution of TMPyP between binding forms as determined by the decomposition of porphyrin absorption spectra. No difference was found in the amount of bound TMPyP between DNAs of various base compositions; however, the ratio of TMPyP binding forms depends on the AT/GC ratio. The presence of protein capsid opposes the binding of TMPyP to DNA. This behavior offers a possibility to investigate the protein capsid integrity due to the analysis of porphyrin binding. Increasing ionic strength of monovalent ions decreases the amount of bound porphyrin through the inhibition of intercalation, but does not influence the quantity of groove-binding forms when TMPyP interacts with isolated DNA. In the case of the nucleoprotein complex the groove-binding is also inhibited already at 140 mM ionic strength. The presence of 1 mM divalent cations (Mg(2+), Ca(2+), Cu(2+) and Ni(2+)) in a buffer solution of 70 mM ionic strength does not influence significantly the free to bound ration of TMPyP when it interacts with isolated DNA. The contribution of binding forms is remarkably different in Mg(2+)/Ca(2+) and Cu(2+)/Ni(2+) containing solutions. Transition metals significantly decrease the binding sites for intercalation in both DNA and nucleoprotein complex, but facilitate the groove-binding of TMPyP to isolated DNA.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号