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951.
Riley KE Murray JS Fanfrlík J Rezáč J Solá RJ Concha MC Ramos FM Politzer P 《Journal of molecular modeling》2011,17(12):3309-3318
In the past several years, halogen bonds have been shown to be relevant in crystal engineering and biomedical applications.
One of the reasons for the utility of these types of noncovalent interactions in the development of, for example, pharmaceutical
ligands is that their strengths and geometric properties are very tunable. That is, substitution of atoms or chemical groups
in the vicinity of a halogen can have a very strong effect on the strength of the halogen bond. In this study we investigate
halogen-bonding interactions involving aromatically-bound halogens (Cl, Br, and I) and a carbonyl oxygen. The properties of
these halogen bonds are modulated by substitution of aromatic hydrogens with fluorines, which are very electronegative. It
is found that these types of substitutions have dramatic effects on the strengths of the halogen bonds, leading to interactions
that can be up to 100% stronger. Very good correlations are obtained between the interaction energies and the magnitudes of
the positive electrostatic potentials (σ-holes) on the halogens. Interestingly, it is seen that the substitution of fluorines
in systems containing smaller halogens results in electrostatic potentials resembling those of systems with larger halogens,
with correspondingly stronger interaction energies. It is also shown that aromatic fluorine substitutions affect the optimal
geometries of the halogen-bonded complexes, often as the result of secondary interactions. 相似文献
952.
House flies were collected from 16 different provinces in the Aegean and Mediterranean regions of Turkey, and the frequencies of pyrethroid resistance-associated mutations in Vssc1 and CYP6D1 in these field-collected populations were studied. Although there is no organized resistance management program for house fly control in Turkey, it is known that different groups of insecticides, including pyrethroids, are used. The frequencies of both Vssc1 and CYP6D1 alleles were weighted toward the susceptibles, with Vssc1-susceptible alleles having higher frequencies in both regions (0.75 in Aegean and 0.69 in Mediterranean populations) than CYP6D1-susceptible alleles (0.65 in Aegean and 0.56 in Mediterranean populations). The frequencies of kdr-his alleles were higher than the frequencies of kdr alleles in these populations. While the frequencies of kdr-his alleles were close to each other in the Aegean (0.23) and Mediterranean (0.17) populations, the frequencies of kdr alleles remarkably differed in these two regions, with values of 0.02 and 0.14, respectively. In contrast to Europe, Asia, and the U.S.A., no super-kdr allele was detected in the samples from both regions. We identified six and eight different Vssc1+CYP6D1 genotype classes in the Aegean and Mediterranean regions, respectively. The three most common genotype classes in the regions were susceptible Vssc1 with heterozygous CYP6D1v1 (29%), sus/kdr-his1 with heterozygous CYP6D1v1 (23%), and susceptible Vssc1 with CYP6D1 (22%). The total frequencies of these three most common genotype classes (approximately 75%) obtained in our study were very close to the value obtained in Florida in a previous study, which was related by the similarity of temperature patterns between Florida and the corresponding regions of Turkey. This may reflect the lack of overwintering fitness cost associated with resistance alleles in both climates. 相似文献
953.
954.
Saldarriaga JF Gile GH James ER Horák A Scheffrahn RH Keeling PJ 《The Journal of eukaryotic microbiology》2011,58(6):487-496
Pseudotrichonympha is a large hypermastigote parabasalian found in the hindgut of several species of rhinotermitid termites. The genus was discovered more than 100 years ago, and although over a dozen species have since been described, this represents only a small fraction of its likely diversity: the termite genera from which Pseudotrichonympha is known are all species rich, and in most cases their hindgut symbionts have not been examined. Even formally described species are mostly lacking in detailed microscopic data and/or sequence data. Using small subunit ribosomal RNA gene sequences and light and scanning electron microscopy we describe here the morphology and molecular phylogenetic position of two Pseudotrichonympha species: the type species for the genus, Pseudotrichonympha hertwigi from Coptotermes testaceus (described previously in line drawing only), and Pseudotrichonympha paulistana from Heterotermes tenuis (described previously based on light microscopy only). 相似文献
955.
Procházková J Kubala L Kotasová H Gudernová I Šrámková Z Pekarová M Sarkadi B Pacherník J 《Free radical research》2011,45(7):779-787
Intracellular production of reactive oxygen species (ROS) plays an important role in the control of cell physiology. For the assessment of intracellular ROS production, a plethora of fluorescent probes is commonly used. Interestingly, chemical structures of these probes imply they could be substrates of plasma membrane efflux pumps, called ABC transporters. This study tested whether the determination of intracellular ROS production and mitochondrial membrane potential by selected fluorescent probes is modulated by the expression and activity of ABC transporters. The sub-clones of the HL-60 cell line over-expressing MDR1, MRP1 and BCRP transporters were employed. ROS production measured by luminol- and L-012-enhaced chemiluminescence and cytochrome c reduction assay showed similar levels of ROS production in all the employed cell lines. It was proved that dihydrorhodamine 123, dihexiloxocarbocyanine iodide, hydroethidine, tetrachloro-tetraethylbenzimidazolocarbo-cyanine iodide and tetramethylrhodamine ethyl ester perchlorate are substrates for MDR1; dichlorodihydrofluoresceine, hydroethidine and tetramethylrhodamine ethyl ester perchlorate are substrates for MRP1; dichlorodihydrofluoresceine, dihydrorhodamine 123, hydroethidine and tetrachloro-tetraethylbenzimidazolocarbo-cyanine iodide are substrates for BCRP. Thus, the determination of intracellular ROS and mitochondrial potential by the selected probes is significantly altered by ABC transporter activities. The activity of these transporters must be considered when employing fluorescent probes for the assessment of ROS production or mitochondrial membrane potential. 相似文献
956.
957.
Since the 1950s, agricultural intensification has affected the structure and functioning of ecological systems including wet
grasslands. Our study site, a wet grassland near Třeboň, Czech Republic, was historically a sedge meadow, but increased nutrient
additions, a long-lasting flood in 2002 and changed mowing patterns resulted in domination by Phalaris arundinacea. The aim of the study was to determine how different nutrient conditions may affect plant structure and production in a wet
grassland used for hay production. Species composition and percent cover were determined from line intercepts. Aboveground
biomass was harvested six times each in 2007 and 2008 and primary production then calculated. Ingrowth core bags were used
to determine belowground production. Aboveground production was two times greater in the high nutrient versus the low nutrient
area in both years, while belowground production was very similar. The high nutrient area was still dominated by P. arundinacea, but Carex gracilis was now a co-dominant in the low nutrient area. However, other factors, such as water level and mowing regime, may interact
with nutrient level to govern wet grassland structure and function. 相似文献
958.
Christian Skov Kim Aarestrup Finn Sivebæk Stig Pedersen Trude Vrålstad Søren Berg 《Biological invasions》2011,13(6):1269-1274
North American signal crayfish (Pacifastacus leniusculus) are invasive in Europe and pose a serious threat to indigenous European crayfish such as the noble crayfish (Astacus astacus). This is mainly because signal crayfish is the carrier of crayfish plague agent, Aphanomyces astaci, which freshwater crayfish from all other continents are highly susceptible to. Until recently, the distribution of signal crayfish in Danish streams has been considered local and restricted to a small geographical area. Here we present data demonstrating that signal crayfish are now widespread in Denmark, including the largest Danish river, River Gudenå. For one of the rivers where co-existing signal crayfish and indigenous noble crayfish were documented, sensitive molecular tests could not detect the crayfish plague agent Aphanomyces astaci in either species. Hence, it seems that not all signal crayfish are chronic carriers of the disease. For the remaining freshwater systems with the introduced signal crayfish, the infection status is presently unknown. Large areas of the freshwater systems in Denmark also remain unexplored with respect to presence/absence of signal crayfish and noble crayfish. Nevertheless, our preliminary data that covers about 14% of the Danish rivers, strongly suggests that signal crayfish should be considered as a common invader that poses an increased threat to the biota in Danish streams, in particular for the indigenous noble crayfish. 相似文献
959.
960.
Exploiting Allee effects for managing biological invasions 总被引:1,自引:0,他引:1
Biological invasions are a global and increasing threat to the function and diversity of ecosystems. Allee effects (positive density dependence) have been shown to play an important role in the establishment and spread of non-native species. Although Allee effects can be considered a bane in conservation efforts, they can be a benefit in attempts to manage non-native species. Many biological invaders are subject to some form of an Allee effect, whether due to a need to locate mates, cooperatively feed or reproduce or avoid becoming a meal, yet attempts to highlight the specific exploitation of Allee effects in biological invasions are surprisingly unprecedented. In this review, we highlight current strategies that effectively exploit an Allee effect, and propose novel means by which Allee effects can be manipulated to the detriment of biological invaders. We also illustrate how the concept of Allee effects can be integral in risk assessments and in the prioritization of resources allocated to manage non-native species, as some species beset by strong Allee effects could be less successful as invaders. We describe how tactics that strengthen an existing Allee effect or create new ones could be used to manage biological invasions more effectively. 相似文献