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901.
The molecular pathogenesis of infections caused by group A Streptococcus (GAS) is not fully understood. We recently reported that a recombinant protein derived from the collagen-like surface protein, Scl1, bound to the human collagen receptor, integrin α2 β1 . Here, we investigate whether the same Scl1 variant expressed by GAS cells interacts with the integrin α2 β1 and affects the biological outcome of host–pathogen interactions. We demonstrate that GAS adherence and internalization involve direct interactions between surface expressed Scl1 and the α2 β1 integrin, because (i) both adherence and internalization of the scl1- inactivated mutant were significantly decreased, and were restored by in-trans complementation of Scl1 expression, (ii) GAS internalization was reduced by pre-treatment of HEp-2 cells with anti-α2 integrin-subunit antibody and type I collagen, (iii) recombinant α2 -I domain bound the wild-type GAS cells and (iv) internalization of wild-type cells was significantly increased in C2C12 cells expressing the α2 β1 integrin as the only collagen-binding integrin. Next, we determined that internalized GAS re-emerges from epithelial cells into the extracellular environment. Taken together, our data describe a new molecular mechanism used by GAS involving the direct interaction between Scl1 and integrins, which increases the overall capability of the pathogen to survive and re-emerge. 相似文献
902.
Technology transfer from worms and flies to vertebrates: transposition-based genome manipulations and their future perspectives
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To meet the increasing demand of linking sequence information to gene function in vertebrate models, genetic modifications must be introduced and their effects analyzed in an easy, controlled, and scalable manner. In the mouse, only about 10% (estimate) of all genes have been knocked out, despite continuous methodologic improvement and extensive effort. Moreover, a large proportion of inactivated genes exhibit no obvious phenotypic alterations. Thus, in order to facilitate analysis of gene function, new genetic tools and strategies are currently under development in these model organisms. Loss of function and gain of function mutagenesis screens based on transposable elements have numerous advantages because they can be applied in vivo and are therefore phenotype driven, and molecular analysis of the mutations is straightforward. At present, laboratory harnessing of transposable elements is more extensive in invertebrate models, mostly because of their earlier discovery in these organisms. Transposons have already been found to facilitate functional genetics research greatly in lower metazoan models, and have been applied most comprehensively in Drosophila. However, transposon based genetic strategies were recently established in vertebrates, and current progress in this field indicates that transposable elements will indeed serve as indispensable tools in the genetic toolkit for vertebrate models. In this review we provide an overview of transposon based genetic modification techniques used in higher and lower metazoan model organisms, and we highlight some of the important general considerations concerning genetic applications of transposon systems. 相似文献
903.
The femoral organ of Zodarion spiders has not been investigated in detail yet. In this study we describe the external and internal structure of this organ. The organ is situated at the distal tip of the femora of all the legs during all developmental stages. The size of the organ (expressed as the number of hairs) increased with the ontogenetic development of Zodarion species. The organ was confirmed to occur in both sexes of all 47 Zodarion species examined. It is possibly present in all species of this genus. The size of the organ increased with the size of the species. A comparative anatomical study was performed in juveniles and adults of both sexes of Zodarion rubidum, and females of both Z. cyrenaicum and Z. jozefienae. The femoral organ represents an exocrine gland composed of a group of secretory cells located below the epidermis. Each gland cell is connected with the leg surface by a single duct. The ducts run in intercellular spaces and specialised canal cells are lacking. The structure of the secretory cells, namely the abundance of smooth endoplasmic reticulum, suggests that the gland produces a volatile compound(s). The composition and the role of the secretion, however, remains unknown. 相似文献
904.
Hydrobiologia - Small streams in the temperate continental region of central Europe have been recently exposed to frequent drying. We investigated the effects of drying on clitellate communities in... 相似文献
905.
Říha Milan Rabaneda-Bueno Ruben Jarić Ivan Souza Allan T. Vejřík Lukáš Draštík Vladislav Blabolil Petr Holubová Michaela Jůza Tomas Gjelland Karl Ø. Rychtecký Pavel Sajdlová Zuzana Kočvara Luboš Tušer Michal Prchalová Marie Seďa Jaromír Peterka Jiří 《Hydrobiologia》2022,849(15):3351-3371
Hydrobiologia - To understand the spatiotemporal overlap in the habitat use of sympatric predators, we studied longitudinal activity and reservoir section and depth use of pike (Esox lucius),... 相似文献
906.
Kuşçu Gökçe Ceren Gürel Çevik Buhur Aylin Oltulu Fatih Akman Levent Köse Timur Yavaşoğlu Nefise Ülkü Karabay Yavaşoğlu Altuğ 《Molecular biology reports》2022,49(3):1721-1729
Molecular Biology Reports - Polycystic ovary syndrome (PCOS) is a metabolic disease that causes infertility due to anovulation in women in reproductive age. It is known that clomiphene citrate (CC)... 相似文献
907.
Demogenetic structure of brown trout Salmo trutta Linnaeus, 1758 populations in mountain headwaters: implications for conservation management
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L. Závorka P. Horký J. Kohout L. Kalous O. Slavík 《Zeitschrift fur angewandte Ichthyologie》2015,31(3):501-508
A demogenetic analysis based on 7 years of observation (2005–2011) was conducted to examine the population structure of brown trout Salmo trutta in pristine dendritic headwaters. The value of genetic divergence (FST) among sampling units ranged from ?0.03 to 0.16. Demographic synchrony was low or moderate, and the average correlation coefficient of population growth between sampling units () ranged from 0.28 to 0.66. No isolation by distance was observed, but genetic divergence was negatively correlated with demographic synchrony among sampling units. Variance in the population growth rate (i.e. local extinction probability) increased with distance from the mainstream and from other sampling units. In contradiction with the usual model of stream‐dwelling salmonids, the upstream sections of headwaters holds only ephemeral subpopulations, whereas the mainstream played a role in the source area of the metapopulation. These findings stress the importance of the mainstream in management conservation for brown trout in low productive mountain headwaters. 相似文献
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