排序方式: 共有71条查询结果,搜索用时 171 毫秒
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Fractionation of proteins from the thylakoid lumen of spinach chloroplasts combined with peptidyl-prolyl cis/trans isomerase (PPIase) measurements revealed a major isomerase activity that was ascribed to a novel enzyme TLP20 (thylakoid lumen PPIase of 20 kDa). TLP20 was inhibited by cyclosporin A and mass spectrometric sequencing of tryptic peptides confirmed its classification as a cyclophilin. Genes encoding similar putative thylakoid cyclophilins with a unique insert of three amino acids NPV in their N-termini were found in chromosome 5 of both Arabidopsis and rice. TLP20 is suggested to be the major PPIase and protein folding catalyst in the thylakoid lumen of plant chloroplasts. 相似文献
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McMullan D Schwarzenbacher R Jaroszewski L von Delft F Klock HE Vincent J Quijano K Abdubek P Ambing E Biorac T Brinen LS Canaves JM Dai X Deacon AM DiDonato M Elsliger MA Eshaghi S Floyd R Godzik A Grittini C Grzechnik SK Hampton E Karlak C Koesema E Kreusch A Kuhn P Levin I McPhillips TM Miller MD Morse A Moy K Ouyang J Page R Reyes R Rezezadeh F Robb A Sims E Spraggon G Stevens RC van den Bedem H Velasquez J Wang X West B Wolf G Xu Q Hodgson KO Wooley J Lesley SA Wilson IA 《Proteins》2004,56(3):615-618
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Maryam Mehdipour Reza Yekta Mina Hanifeh Ahagh Majid Mahdavi Zarrin Ghasemi 《Nucleosides, nucleotides & nucleic acids》2019,38(2):101-118
Stilbene derivatives have been found to possess promising anticancer activities against human cancer cell lines in vitro. In the present study, we have investigated cytotoxic, apoptosis induction and DNA binding activity of new stilbene derivative, (E)-1-(4-Chlorophenyl)-4,5-diphenyl-2-[4-(4-methoxystryl)phenyl]-1H-imidazol (STIM) on K562 chronic myeloid leukemia cell line. Via MTT assay STIM demonstrated cytotoxic activity against K562 cell line with IC50 value of 150?µM. Apoptosis, as the mechanism of cell death, was evaluated by morphological study and flow cytometric analysis. In vitro DNA binding property of STIM has been studied by vital spectroscopic techniques, which indicated that STIM interact with ctDNA through groove binding mode and binding constant (Kb) was estimated to be 6.9?×?104?M?1. Docking studies revealed that hydrophobic is the most important interaction in STIM-DNA complex, and that the ligand (STIM) interacts with DNA via groove binding mode and the bindiyspng energy was calculated as ?13.37?kcal/mol. Taken together, the present study suggests that STIM exhibits anticancer effect on K562 cell line through the induction of apoptosis as well as cell cycle arrest at Sub-G1 phase and also can bind to double helix DNA in vitro. 相似文献
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Mycetomas are the subcutaneous and relatively rare chronic pustular infections. The etiologic agents of mycetomas are a group
of saprophytic fungi and actinomycetes living in soil. We retrospectively discussed the overall prevalence of mycetomas and
the prevalence of infective agents in Iran between 1972 and 2005. Seventy-six cases of mycetomas have been reported from various
geographical locations in Iran during 33 years. Analysis of the records revealed that 84.5% were actinomycetoma and only 15.5%
were eumycetoma. Disease mainly has been seen in foot, and the male to female ratio was 2:1. Mycetomas were abundant among
farmers in rural areas of Iran. The commonest agents of mycetomas were Nocardia asteroids, Actinomadura
madura (actinomycetoma) and Allesheria boydii (eumycetoma). The peak age of onset was between 31 and 51 years. 相似文献
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Seyed Mohammad Bagher Hashemi Fakhri Shahidi Seyed Ali Mortazavi Elnaz Milani Zarrin Eshaghi 《Probiotics and antimicrobial proteins》2014,6(1):22-31
In this study, the probiotic potential of Lactobacillus strains isolated from traditional Kurdish cheese was investigated. The Lactobacillus strains were examined for resistance to gastric acidity and bile toxicity, antimicrobial activities, autoaggregation, coaggregation, hydrophobicity, adhesion to Caco-2 cells, and antibiotic susceptibility. The results showed that all strains tested tolerate acid gastric conditions (pH 2.0 and 3.0), and all of them were bile resistant (at 0.3 and 1 % concentration). Although no antibacterial activity was detected in vitro assay for the treated (neutralized to pH 6.5 and treated with catalase) cell-free culture supernatant (CFCS) of strains, untreated CFCS showed strong antagonistic activity against two known pathogens bacteria. All strains exhibited a strong autoaggregating phenotype and manifested a high degree of coaggregation with pathogens. On the other hand, majority of studied strains were found sensitive to different antibiotics, such as ampicillin, penicillin, ciprofloxacin, chloramphenicol, erythromycin, rifampicin, and tetracycline, and were resistant to vancomycin and streptomycin. Finally, isolated strains showed good hydrophobicity and adherence to Caco-2 cell line, so they could be exploited for food manufacture. 相似文献
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Ghahremanifard Parisa Afzali Farzaneh Rostami Amin Nayeri Zahra Bambai Bijan Minuchehr Zarrin 《International journal of peptide research and therapeutics》2020,26(2):1127-1136
International Journal of Peptide Research and Therapeutics - Hepatocellular carcinoma (HCC) is one of the leading cancer-related deaths worldwide. Recently, studies for HCC treatment are focused on... 相似文献
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