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排序方式: 共有98条查询结果,搜索用时 15 毫秒
41.
AM Zakharenko MI Kusaykin SN Kovalchuk VV Sova AS Silchenko AA Belik SD Anastyuk BM Ly VA Rasskazov TN Zvyagintseva 《Biochemistry. Biokhimii?a》2012,77(8):878-888
A specific 1→3-β-D-glucanase with molecular mass 37 kDa was isolated in homogeneous state from crystalline style of the commercial marine mollusk Tapes literata. It exhibits maximal activity within the pH range from 4.5 to 7.5 at 45dgC. The 1→3-β-D-glucanase catalyzes hydrolysis of β-1→3 bonds in glucans as an endoenzyme with retention of bond configuration, and it has transglycosylating activity. The K m for hydrolysis of laminaran is 0.25 mg/ml. The enzyme is classified as a glucan endo-(1→3)-β-D-glucosidase (EC 3.2.1.39). The cDNA encoding this 1→3-β-D-glucanase from T. literata was sequenced, and the amino acid sequence of the enzyme was determined. The endo-1→3-β-D-glucanase from T. literata was assigned to the 16th structural family (GHF 16) of O-glycoside hydrolases. 相似文献
42.
Shubenkova O. V. Zakharenko A. S. Galach’yants Yu. P. Kalmychkov G. V. Ivanov V. G. Khabuev A. V. Pogodaeva T. V. Chernitsyna S. M. Zemskaya T. I. 《Microbiology》2021,90(4):443-454
Microbiology - Diversity of methano- and methylotrophic bacteria in enrichments from the oxidized sediment layer maintained at 10°C in mineral media with nitrogen compounds (... 相似文献
43.
N V Timakova G I Aleshkin I V Titova M G Goderdzishvili V I Zakharenko G V Brukhanski? O G Loginova S Ia Ditiatkin B N Il'iashenko 《Biulleten' eksperimental'no? biologii i meditsiny》1990,109(3):299-301
The transcipients were obtained in intrageneric matings of E.coli donor harbouring the plasmid PR4::Mu cts 62 with Bac. cereus GP7 recipient cells with the frequency 10(-9). The transcipient clone Bac. cereus 682 was selected having stably inherited and expressed the hybrid plasmid PR4::Mu cts 62 genes for antibiotic resistance and temperature sensitivity. Production of the bacteriophage Mu cts 62 particles was not registered in the bacillary transcipient cells. The plasmid RP4::Mu cts 62 genes were localized in the chromosome of Bac. cereus 682 transcipient by the blot-hybridization technique with 32P-labelled DNA of the bacteriophage Mu cts 62 and the plasmid PR4. The transcipient of Bac. cereus 682 has the donor properties and transfers the RP4::Mu cts 62 genes to recipient cells of Bac. cereus DSM 318 with the frequency of 10(-6)-10(-7). The expression and transfer of the gram-negative plasmid genes in gram-positive bacterial cells are discussed. 相似文献
44.
D A Mel'nichuk N A Zakharenko N M Shabel'nik T V Liubetskaia G Ia Chizhskaia 《Ukrainski? biokhimicheski? zhurnal》1985,57(3):44-49
Activation possibilities of redox and biosynthetic processes are studied for lactic cows with their ration supplemented by mineral-ammonium preparation MII-15. In the blood of cows from the experimental group the amount of a number of glycolysis, biocarboxylic cycle, ammonium nitrogen exchange products as well as activity of overamination enzymes vary as compared to control, which testifies to the stimulation of redox processes and protein exchange in tissues. Application of the MII-15 preparation is accompanied by an increase in the lactic productivity of cows by 8-10% on the average. 相似文献
45.
The 3D-FISH was employed to investigate the telomere topology in polytene nuclei of salivary glands of Drosophila melanogaster. The majorities of telomeres in polytene nuclei of salivary glands in Drosophila strain y(2-717) are localized in the nuclear central area and have no contacts with nuclear membrane. In females of this strain, ectopic contacts between telomeres occur at 25 % higher frequency than in males. HeT-A DNA in y(2-717alk3-2) strain, which is a derivative of y(2-717) carrying an inversion between 1D and 13C bands, is found in region 13 of X chromosome. The frequency of ectopic contacts of telomeres in y(2-717alk3-2) males is 10 % higher than that in y(2-717) strain. The number of ectopic contacts can be significantly different in independent experiments, possibly indicating the role of random factors in the contact formation. 相似文献
46.
Kolodkina VL Fel'dman EV Dronina AM Titov LP Kozhemiakin AK Zakharenko DF 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1999,(4):34-38
The outbreak of diphtheria in the Republic of Belarus developed in the presence of a sharp deficiency of immunity among the population aged 35 years and over. In connection with a higher initial immunity level resulting from vaccination made in one injection the minimum antibody level (> or = 0.01 I.U./ml) was found in 79.7% of the examinees among the population under 35 years. For the age group of 35-60 years vaccination in one and two injections proved to be insufficiently effective (the minimum protective antibody titer was found, respectively, in 64.3% and 63.3% of the vaccinees). To achieve the full protection of this group of the population, vaccination in three injections was necessary; after that the minimum protective antibody titer was found in 90.7%, the protective antibody titer (0.1 I.U./ml) was found in 53.5% and the highly protective antibody titer (1.0 I.U./ml) was found in 11.6% of the examinees. The low level of highly protected persons among vaccinees receiving three injections was due to a low dose of diphtheria toxoid (2 Lf) in adsorbed DT-m vaccine with reduced antigen content. 相似文献
47.
Sitnikova Tatiana Ya. Naumova Tatiana V. Mekhanikova Irina V. Kiyashko Sergey I. Kalmychkov Gennady V. Karanovic Ivana Zakharenko Alexandra S. Bukin Yuri S. Khabuev Andrey V. Ivanov Vyacheslav G. Khlystov Oleg M. Zemskaya Tamara I. 《Hydrobiologia》2022,849(8):1947-1968
Hydrobiologia - The biological traits of invertebrates inhabiting freshwater methane seeps are poorly understood. We analysed the relationship between invertebrate abundance and environmental... 相似文献
48.
Chepanova A. A. Li-Zhulanov N. S. Sukhikh A. S. Zafar A. Reynisson J. Zakharenko A. L. Zakharova O. D. Korchagina D. V. Volcho K. P. Salakhutdinov N. F. Lavrik O. I. 《Russian Journal of Bioorganic Chemistry》2019,45(6):647-655
Russian Journal of Bioorganic Chemistry - Tyrosyl-DNA phosphodiesterase 1 (Tdp1) is one of the important DNA repair enzymes responsible for the repair of DNA damage caused by anticancer drugs, such... 相似文献
49.
The effects of genotype of the laboratory strains, C(1)DX, ywf/Y, 23.5 MRF/CyL 4, and C(1)DX,yf; π2, on locus-specific instability in the yellow gene of the strains y 2-217, y 2-715, and y 2-700 from Uman’ population of Drosophila melanogaster was studied. Crosses of the males from Uman’-derived lines with the C(1)DX,ywf/Y females yielded a cascade of derivatives, mostly consisting of y + and y 2 alleles, while their crosses with the 23.5 MRF/CyL 4 and C(1)DX,yf; π2 females mostly resulted in the appearance of y + and y 1 derivatives. The genomes of laboratory strains used in the study contained the full-sized hobo elements, which could differ from one another relative to the structure of variable region and affinity to different DNA sequences. 相似文献
50.
L. P. Zakharenko M. P. Perepelkina O.V. Antonenko O. V. Vykhristyuk V. M. Efimov L. A. Vasil’eva 《Cell and Tissue Biology》2011,5(5):428-439
The effect of selecting the length of a radial vein on the distribution of hybridization sites for P and hobo transposons and mdg1 and mdg2 retrotransposons on polytene chromosomes of Drosophila melanogaster salivary glands was studied. The pattern of the transposable element (TEs) distribution was polymorphic in both parental
and selected strains. The similarity in mdg1 and mdg2 distribution between strains selected in one direction was closer than between strains selected in the opposite direction,
but the selected strains were closer to each other than to the parental strain, regardless of direction of selection. No new
mdg2 hybridization sites that would be absent from the control were found in the selected strains compared to the control. The
number of mdg1 and hobo hybridization sites was more selected in strains in the (+) direction than in the (−) direction. The mobility of hobo copies in the strains correlated with the presence of its full-sized copy in the genome. The polymorphism of all TEs studied
except for mdg1 was higher in strains selected in the (+) direction than in the (−) direction. These results suggest that some TEs migrate
over the genome independently of selection, whereas the other are markers of evolutionary events rather than their causes. 相似文献