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161.
Grebennikova TV Zaberezhnyĭ AD Vlasova AN Musienko MI Sokolov MA Grabovetskiĭ VV Tsybezov VV Bogdanova VS Orliankin BG Aliper TI Nepoklonov EA 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》2004,(2):37-40
The porcine reproductive and respiratory syndrome (PRRS) is a contagious viral pathology caused by PRRS virus. There are 2 types of the above virus--the European and American ones. Distribution patterns of the PRRS virus were studied for Russia and Byelorussia. Above 700 porcine sera obtained from 32 households of 21 Russia's administrative regions and from 19 households of 6 Byelorussia's administrative regions were tested for presence of antibodies to the PRRS virus. Simultaneously, the samples were tested for virus presence by polymerase chain reaction (PCR). It was proven serologically that the PRRS virus is widespread in the territories of Russia and Byelorussia. Noteworthily, all field isolates found in Russia and Byelorussia belong to the European type. Not a single viral isolate of the American PRRS type was found. The nucleocapsid (N) recombinant protein was obtained on the basis of the Russian field isolate of the PRRS virus by using the E. coli. expression system. Finally, it was shown as possible to use the recombinant protein in indirect immune enzyme assay for the sake of detecting the antibodies to the PRRS virus. 相似文献
162.
Holovats'kyĭ ID 《Ukrainski? biokhimicheski? zhurnal》2004,76(2):133-137
The 150th anniversary of birth of Ivan Yakovych Horbachevsky (15.05.1854-24.05.1942) is celebrated in 2004. He was a well-known biochemist and epidemiologist, professor of medical chemistry of the Vienna University, Czech University of Prague from 1883 (rector--from 1902), Member of the Academy of Sciences of the Ukr.SSR (sinse 1925), the well-known public figure. His main works belonged to the field of physiological chemistry. I. Ya. Horbachevsky was the first to synthesize uric acid (1882), he established sources and methods of its formation in the organism. He established that amino acids are the components of proteins, discovered the enzyme xanthine oxidase, performed synthesis of creatine, investigated vitamins, found out the causes of pellagra, studied the problems of nutrition of Halychina population. Professor I. Ya. Horbachevsky is the author of the manual in physiological chemistry in four volumes which he wrote in Czech (1904-1908) and in organical chemistry in Ukrainian (1924). He initiated the creation of Ukrainian chemical terminology. 相似文献
163.
Selenium is essential trace element, sulphur analogue with high chemical activity, component of some selenoproteins and enzymes: glutathione peroxidase and other peroxidases, blood and tissue proteins. As to their biological action mechanism selenium and its compounds are antioxidants. Selenium is active immunomodulator, much more potent anti-oxidant than vitamins E, C and A, beta-carotene, but much more toxic. It takes part in thyroxine conversion to triiodethyronine in thyroid hormone biosynthesis. As sperm antioxidant selenium protected its motility and fertility. Selenium is a serious factor of biological and antioxidant protection of vascular endothelium, of low-density lipoproteins, protection of DNA, chromosomes. As food component selenium is an exceptional agent of protection from atherosclerosis, coronary ischemic disease and cancer. Some hydrobionts, liver, kidney, meal, corn and garlic, onion, cabbage, broccoli are dietary products with high content of selenium. 相似文献
164.
The aim of the research consisted in the study of influence of beta-radiation on response of erythrocyte surface potential to inhibitors of eicosanoid metabolism enzymes (cyclo-, lipoxygenase and phospholipase A2). It was shown, that inhibitors of phospholipase A2 (quinacrine, 10-100 microM), cyclooxygenase (aspirin, 10-100 microM) and cyclo- and lipoxygenase (BW755c, 1-100 microM) lowered electrophoretic mobility (EPM) of erythrocytes by 20-30%. An analogous effect can be exerted by beta-radiation. Nonradioactive leucine in the studied concentrations cannot simulate EPM erythrocytes. Response of cellular EPM to these inhibitors depended on their concentration in the incubation medium. Addition of 14C to the incubation medium changed response of EPM of cells to inhibitors of cyclo- and lipoxygenase but not to quinacrine. However beta-radiation fully abolished the stimulative action of nonspecific activator of phospholipase A2 (Ca-independent), H2O2, on cellular EPM. Under these conditions beta-radiation enhanced EPM response to aspirin only at concentration of 100 microM. The EPM response to BW755c is reduced by irradiation at all concentrations with the exception of equal-effective one (10 microM). Data obtained evidence for modification of eicosanoid metabolism by beta-radiation, probably, as a result of phospholipase A2 inhibition, as evident from elimination by radiation of stimulated action of hydrogen peroxide on EPM. The radiation action can also affect the cyclooxygenase lipoxygenase activity ratio, this activity being mediated by cellular membrane signaling systems. 相似文献
165.
Gubs'kyĭ IuI Ianits'ka LV Velykyĭ MM Kuchmerovs'ka TM 《Ukrainski? biokhimicheski? zhurnal》2004,76(6):106-110
Alterations of Na+,K+-ATPase activity and serotoninergic system functioning were investigated in brain synaptosomes fractions of rats under experimental acute 1,2-dichloroethane (DChE) intoxication. It was shown that Na+,K+-ATPase activity was markedly increased (by 41,8%) in a period of 24 h after DChE intoxication and decreased (by 27%) after 48 h intoxication. The level of [2-14C]-serotonin uptake by synaptosomes was progressively diminished after 24 and 48 h after DChE injection whereas the activity of monoamine uptake proved to be unchanged. Nicotinamide (200 mg/kg of body weight) was administered to rats subjected to DChE 1, 24 and 36 h after poisoning. The treatment of rats with nicotinamide resulted in some normalization of brain synaptosomal Na+, K+-ATPase activity and serotonin uptake controlled at 48 h after DChE intoxication. 相似文献
166.
The analysis of natural foci of haemorrhagic fever with renal syndrome (HFRS) and tick-borne borreliosis on the territory of the Ulyanovsk region is presented. Reduced rat control interventions result in a wide spread of these and other infections with natural focality. Monitoring of natural foci of HFRS, tick-borne borreliosis and arbovirus infections, their typing and determination of main reservoirs are the most promising among epidemiological surveillance methods. 相似文献
167.
Osipova IG Makhaĭlova NA Sorokulova IB Vasil'eva EA Gaĭderov AA 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2003,(3):113-119
Data on probiotic preparations prepared from spore-forming bacteria is reviewed. Information on indications for use, effectiveness and the mechanism of action is given. Preparations being produced in Russia (Biosporin, Sporobacterin and Bactosporin) are evaluated in detail as well as the prospects of the application of probiotics from spore-forming bacteria. 相似文献
168.
Detection of Treponema pallidum DNA and RNA in clinical material from patients with syphilis at different stages 总被引:2,自引:0,他引:2
169.
Kirillov DA Chaĭnikova IN Perunova NB Chelpachenko OE Pan'kov AS Smoliagin AI Valyshev AV 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2003,(4):74-78
The effect of the synthetic immunomodulator polyoxydonium (PO) on some biological properties of pathogenic bacteria (Shigella flexneri, Salmonella enteritidis), opportunistic bacteria (Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacteroides fragilis, Peptostreptococcus anaerobius, Prevotella melaninogenica, Propionibacterium propionicum, Clostridium difficile) and fungi (Candida albicans), isolated during enteric infections, enteric dysbiosis, pyoinflammatory diseases, was evaluated in a number of in vitro experiments. The study revealed that the recommended therapeutic concentration of PO decreased antilysozyme activity (ALA) and the anticomplement activity in Klebsiella, Shigella, Propionibacterium, Clostridium, bacteroids, fungi of the genus Candida, but increased ALA in nonhemolytic Escherichia. Under the action of PO an increased sensitivity of the microorganisms under study to definite antibiotics of the lincosamide, fluoroquinolon, carbapenem and cephalosporin groups was noted. The data obtained in this study reveal one of the possible mechanisms of the corrective action of PO on the microbiocenosis of the intestine in dysbiosis, enteric infections and pyoinflammatory diseases. 相似文献
170.
Tuĭgunov MM Gabidullin ZG Zurochka AV Bukharin OV 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2003,(4):23-27
Modern data on the molecular mechanisms of relationships between the host organism and the pathogenic representatives of the family Enterobacteriaceae in the host-parasite system are presented. The process of cytokine and eicosanoid regulation of the immune process of the host in the norm and pathology states are analyzed. The examples of the mechanisms of immune suppression, false antigenic stimulation and the mimicry of pathogens are given. 相似文献