全文获取类型
收费全文 | 93400篇 |
免费 | 146003篇 |
国内免费 | 42628篇 |
专业分类
282031篇 |
出版年
2021年 | 2404篇 |
2020年 | 2494篇 |
2019年 | 4147篇 |
2018年 | 3143篇 |
2017年 | 2946篇 |
2016年 | 3058篇 |
2015年 | 3783篇 |
2014年 | 4638篇 |
2013年 | 4150篇 |
2012年 | 5499篇 |
2011年 | 5967篇 |
2010年 | 7114篇 |
2009年 | 12398篇 |
2008年 | 6520篇 |
2007年 | 6205篇 |
2006年 | 5280篇 |
2005年 | 4764篇 |
2004年 | 4103篇 |
2003年 | 3433篇 |
2002年 | 4081篇 |
2001年 | 5245篇 |
2000年 | 2986篇 |
1999年 | 7227篇 |
1998年 | 9115篇 |
1997年 | 9190篇 |
1996年 | 8564篇 |
1995年 | 8814篇 |
1994年 | 8210篇 |
1993年 | 7859篇 |
1992年 | 7779篇 |
1991年 | 7817篇 |
1990年 | 8638篇 |
1989年 | 7899篇 |
1988年 | 7188篇 |
1987年 | 6275篇 |
1986年 | 5806篇 |
1985年 | 5246篇 |
1984年 | 4045篇 |
1983年 | 3265篇 |
1982年 | 3600篇 |
1981年 | 3233篇 |
1980年 | 3154篇 |
1979年 | 3247篇 |
1978年 | 2967篇 |
1977年 | 2897篇 |
1976年 | 2722篇 |
1974年 | 2459篇 |
1973年 | 2464篇 |
1972年 | 2811篇 |
1971年 | 2590篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
992.
Localization of an alpha-amanitin resistance mutation in the gene encoding the largest subunit of mouse RNA polymerase II. 总被引:20,自引:7,他引:13 下载免费PDF全文
RNA polymerase II is inhibited by the mushroom toxin alpha-amanitin. A mouse BALB/c 3T3 cell line was selected for resistance to alpha-amanitin and characterized in detail. This cell line, designated A21, was heterozygous, possessing both amanitin-sensitive and -resistant forms of RNA polymerase II; the mutant form was 500 times more resistant to alpha-amanitin than the sensitive form. By using the wild-type mouse RNA polymerase II largest subunit (RPII215) gene (J.A. Ahearn, M.S. Bartolomei, M. L. West, and J. L. Corden, submitted for publication) as the probe, RPII215 genes were isolated from an A21 genomic DNA library. The mutant allele was identified by its ability to transfer amanitin resistance in a transfection assay. Genomic reconstructions between mutant and wild-type alleles localized the mutation to a 450-base-pair fragment that included parts of exons 14 and 15. This fragment was sequenced and compared with the wild-type sequence; a single AT-to-GC transition was detected at nucleotide 6819, corresponding to an asparagine-to-aspartate substitution at amino acid 793 of the predicted protein sequence. Knowledge of the position of the A21 mutation should facilitate the study of the mechanism of alpha-amanitin resistance. Furthermore, the A21 gene will be useful for studying the phenotype of site-directed mutations in the RPII215 gene. 相似文献
993.
O K Haffar A K Vallerga S A Marenda H J Witchel G L Firestone 《Molecular and cellular biology》1987,7(4):1508-1517
994.
995.
Unusual sequence element found at the end of an amplicon. 总被引:17,自引:1,他引:16
In a polyomavirus-transformed rat cell line, designated LPT, the polyomavirus DNA is integrated into a single chromosomal site. Treatment of LPT cells with carcinogens induces amplification of the integrated virus DNA and flanking cellular sequences. We show that the amplification is arrested within a specific cell DNA segment that maps 1.3 to 1.85 kilobases beyond one virus-cell DNA junction, defined as the left junction. We also present the sequence of an 897-base-pair fragment spanning the arrest site. This fragment contains an unusual sequence element, which consists of two contiguous components, a potential cruciform with stems of 6 base pairs and a d(G-A)27 X d(T-C)27 tract, and maps 1,497 to 1,564 nucleotides beyond the left junction. The possibility that this unusual sequence plays a role in the arrest of the amplification process is discussed. 相似文献
996.
997.
Alterations in pp60c-src accompany differentiation of neurons from rat embryo striatum. 总被引:17,自引:9,他引:8
C A Cartwright R Simantov P L Kaplan T Hunter W Eckhart 《Molecular and cellular biology》1987,7(5):1830-1840
Cultured neurons from rat embryo striatum were found to contain two structurally distinct forms of pp60c-src. The 60-kilodalton (kDa) form appeared similar to pp60c-src from cultured rat fibroblasts or astrocytes. The 61-kDa form was specific to neurons and differed in the NH2-terminal 18 kDa of the molecule. In undifferentiated neurons the predominant phosphorylated species of pp60c-src was the fibroblast form. Upon differentiation, a second phosphorylated form of pp60c-src was detected. This form had two or more additional sites of serine phosphorylation within the NH2-terminal 18-kDa region of the molecule, one of which was Ser-12. The specific protein-tyrosine kinase activity of the total pp60c-src population increased 14-fold, as measured by autophosphorylation, or 7-fold, as measured by phosphorylation of an exogenous substrate, as striatal neurons differentiated. This elevation in protein kinase activity occurred without a detectable decrease in Tyr-527 phosphorylation or increase in Tyr-416 phosphorylation. Our results support the idea that the expression of the neuron-specific form of pp60c-src and the increase in specific protein kinase activity may be important for neuronal differentiation. 相似文献
998.
DNA sequence analysis of spontaneous mutations at the aprt locus of hamster cells. 总被引:13,自引:3,他引:10 下载免费PDF全文
To determine the nature of spontaneous mutational events in cellular genes in hamster cells, mutant adenine phosphoribosyltransferase (aprt) genes were cloned and the regions to which we mapped alterations were sequenced. A variety of nucleotide changes were found to occur in the 12 mutant genes analyzed. Most mutations were simple base-pair substitutions-transitions (both G X C----A X T and A X T----G X C) and transversions. The only multiple mutation was a simple transition next to a single-base-pair insertion. Of the 12 mutations, 4 were more complex, involving small deletions or duplications. Two of these were similar to previously described deletions in that they occurred between short direct sequence repeats. No hot spots were detected. Three independent mutations were characterized at one restriction endonuclease site, although no other mutations were detected in the nucleotides surrounding this site in other mutant strains. At a functional level, sequence changes were either in exons (resulting in missense and, in one instance, nonsense mutations) or at splicing sites. 相似文献
999.
1000.