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61.
用固相pH梯度(pH 5.05~5.60)等电聚焦技术对随机抽取的188名北京地区健康汉族人的血清转铁蛋白(Tf)进行分型调查,并统计基因频率,检出了在中国还未见报道的TfC3基因.其表型分别是:TfC1, TfC2, TfC1C2, TfC1C3, TfC1Dchi, TfC2Dchi.TfC1=0.7420, TfC2=0.2420, TfC3=0.0027, TfDchi=0.0133, 符合Hardy-Weinberg定律, 并与其他已见报道的汉族 Tf基因频率大致相符. 相似文献
62.
Purification and characterization of 2,6-dichloro-p-hydroquinone chlorohydrolase from Flavobacterium sp. strain ATCC 39723. 总被引:1,自引:0,他引:1
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The biochemistry of pentachlorophenol (PCP) degradation by Flavobacterium sp. strain ATCC 39723 has been studied, and two enzymes responsible for the conversion of PCP to 2,6-dichloro-p-hydroquinone (2,6-DiCH) have previously been purified and characterized. In this study, enzymatic activities consuming 2,6-DiCH were identified from the cell extracts of strain ATCC 39723. The enzyme was purified to apparent homogeneity by a purification scheme consisting of seven steps. Gel filtration chromatography showed a native molecular weight of about 40,000, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a single protein of 42,500 Da. The purified enzyme converted 2,6-DiCH to 6-chlorohydroxyquinol (6-chloro-1,2,4-trihydroxybenzene), which was easily oxidized by molecular oxygen and hard to detect. The end product, 6-chlorohydroxyquinol, was detected only in the presence of a reductase and NADH in the reaction mixture. The enzyme dechlorinated 2,6-DiCH but not 2,5-DiCH. The enzyme required Fe2+ for activity and was severely inhibited by metal chelating agents. The optimal conditions for activity were pH 7.0 and 40 degrees C. The Kcat for 2,6-DiCH was 35 microM, and the kcat was 0.011 s-1. 相似文献
63.
Sequence variation and genetic diversity in the giant panda 总被引:3,自引:0,他引:3
ZHANG YapingOliver A. RyderFAN Zhiyong ZHANG HemingHE TingmeiHE Guangxin ZHANG Anju FEI LisongZHONG Shunlong CHEN HongZHANG Chenglin YANG Minghai ZHU Feibing PENG Zhenxin PU Tianchun CHEN Yucun YAO OMinda GUO Wei 《中国科学:生命科学英文版》1997,40(2):210-216
About 336–444 bp mitochondrial D-loop region and tRNA gene were sequenced for 40 individuals of the giant panda which were
collected from Mabian, Meigu, Yuexi, Baoxing, Pingwu, Qingchuan, Nanping and Baishuijiang, respectively. 9 haplotypes were
found in 21 founders. The results showed that the giant panda has low genetic variations, and that there is no notable genetic
isolation among geographical populations. The ancestor of the living giant panda population perhaps appeared in the late Pleistocene,
and unfortunately, might have suffered bottleneck attacks. Afterwards, its genetic diversity seemed to recover to some extent.
Project supported by the “8.5” Key Project of Chinese Academy of Sciences, the Chairman Foundation of Chinese Academy of Sciences,
K. C. Wang Education Foundation, the Applied Basic Research Foundation of Yunnan, the National Natural Science Foundation
of China, the Special Foundation for Returned Chinese Scientists, and Zoological Society of San Diego. 相似文献
64.
Purification and Properties of Component B of 2,4,5-Trichlorophenoxyacetate Oxygenase from Pseudomonas cepacia AC1100 总被引:3,自引:1,他引:2
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Pseudomonas cepacia AC1100 degrades 2,4,5-trichlorophenoxyacetate (2,4,5-T), an herbicide and chlorinated aromatic compound. Although some progress has been made in understanding 2,4,5-T degradation by AC1100 by molecular analysis, little is known about the biochemistry involved. Enzymatic activity converting 2,4,5-T to 2,4,5-trichlorophenol in the presence of NADH and O(inf2) was detected in cell extracts of AC1100. Phenyl agarose chromatography of the ammonium sulfate-fractionated cell extracts yielded no active single fractions, but the mixing of two fractions, named component A and component B, resulted in the recovery of enzyme activity. Component B was further purified to homogeneity by hydroxyapatite and DEAE chromatographies. Component B had a native molecular weight of 140,000, and it was composed of two 49-kDa (alpha)-subunits and two 24-kDa (beta)-subunits. Component B was red, and its spectrum in the visible region had maxima at 430 and 560 nm (shoulder), whereas upon reduction it had maxima at 420 (shoulder) and 530 nm. Each mole of (alpha)(beta) heterodimer contained 2.9 mol of iron and 2.1 mol of labile sulfide. These properties suggest strong similarities between component B and the terminal oxygenase components of the aromatic ring-hydroxylating dioxygenases. Component A was highly purified but not to homogeneity. The reconstituted 2,4,5-T oxygenase, consisting of components A and B, converted 2,4,5-T quantitatively into 2,4,5-trichlorophenol and glyoxylate with the coconsumption of NADH and O(inf2). 相似文献
65.
本文记述分布于我国陕西太白山的园蛛科Araneidae尖腹蛛属Aculeperia一新种,太白尖腹蛛Aculeperiataibaishanensissp.nov.,模式标本分别保存河北教育学院和西安师范学院。 相似文献
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69.
大鼠尾核头部对中缝大核神经元单位活动的影响及其可能途径 总被引:1,自引:0,他引:1
用玻璃微电极细胞外记录大鼠中缝大核(NRM)神经元的单位放电。共记录277个细胞,NRM 神经元自发放电频率大都在每秒0.5—20次之间,平均为6.41 Hz。其中221个神经元被电刺激尾所激活,35个被抑制,21个无明显变化。NRM 神经元对躯体刺激的反应类型与自发放电的特征有关,兴奋型神经元的自发放电频率较低((?)=4.96Hz),而抑制性神经元的自发放电频率较高((?)=15.03 Hz)。在24例兴奋型神经元中,刺激尾核头部能够激活大多数 NRM 神经元的自发放电和抑制其伤害感受性反应。导水管周围灰质微量注射纳洛酮(2.5ug/0.5μl,n=15)。能够明显阻断刺激尾核头部激活 NRM 神经元自发放电和抑制伤害感受性反应的效应。 相似文献
70.
本文记载散白蚁两个新种。模式标本保存于广东省昆虫研究所。 1.蟹腿散白蚁Reticulitermes cancrifemuris新种 兵蚁(图1,a—g;表1—2):体中型,头部橙黄色,上颚黄褐色,颚基色较浅,触角、胸部橙黄色,腹部色较淡,体被密毛。 头长方形,长大于宽,两侧平行,中后部微扩张,后侧角圆匀。额峰隆起,高出头水平 相似文献