组胺H2受体拮抗剂对骨髓造血重建的影响

用组胺H_2受体拮抗剂(甲氰咪胍或呋喃硝胺)处理正常和亚致死量γ-射线照射小鼠,探讨正常体内造血和再生骨髓中造血重建与组胺受体的关系。发现非毒性剂量的甲氰咪胍对正常小鼠骨髓多能造血干细胞(CFU-s)无抑制作用,但可抑制小鼠体内粒单系祖细胞(CFU-GM)的生长和亚致死量照射后CFU-s产率的恢复。组胺可能与骨髓的再生有关,组胺H_2受体拮抗剂可抑制骨髓的造血重建。     

氨甲酰胆碱和Impromidine对CFU—S细胞周期的影响

正常情况下处于S期的CFU-S比例低于10％。氨甲酰胆碱(Cach 10~(-13)—10~(-9)mol/L)和Impromidine(Impro 10~(-9)—10~(-4)mol/L)在体外与小鼠骨髓细胞短时培育后,增加了CFU-S对细胞毒剂羟基脲的敏感性。反应最大时,9d和13dCFU-S的减少率分别是32.8和60.6％(Cach)以及38.4和49.5％(Impro)。这种效应可分别被胆碱能N受体阻断剂筒箭毒(10~(-6)mol/L)和组胺H_2受体阻断剂甲氰咪呱(10~(-6)mol/L)所阻断,表明9d和13d CFU-S表面胆碱能N受体和组胺H_2受体的密度或活性存在差别,再次证实了CFU-S是一个不均一的细胞群。     

华南绒茧蜂属二新种（膜翅目：茧蜂科）

本文报道采自广西和江西的绒茧蜂属二新种。模式标本存于湖南农学院昆虫标本室。1.黄足绒茧蜂Apanteles vitellinipes,新种(图1～4) 雌 体黑色。触角柄节、梗节、下颚须、翅基片、足(包括基节)、腹部第一背板基部凹陷部分、第1～3背板侧膜边缘和产卵管黄色;触角鞭节、前翅前缘脉、翅痣、痣后脉、产卵管鞘深褐色;其它翅脉褐色。     

猴头菇对小鼠抗疲劳作用的实验研究

分别以猴头菇干粉（猴头菇Ⅰ组）和猴头菇浸出液（猴头菇Ⅱ组）饲喂小鼠,观察猴头菇对小鼠血清乳酸脱氢酶（ＬＤＨ）活力、血乳酸、血清尿素氮（ＢＵＮ）、肝糖原、肌糖原含量及运动耐力的影响。结果表明：实验６０ｄ后,猴头菇Ⅰ、Ⅱ组ＬＤＨ活力、肝糖原及肌糖原含量明显高于对照组（Ｐ＜０．０５或Ｐ＜０．０１）;运动后血乳酸的水平和ＢＵＮ的增量明显低于对照组（Ｐ＜０．０５或Ｐ＜０．０１）;运动后血乳酸消除速率显著高于对照组（Ｐ＜０．０５）;在运动耐力测定时在水中淹死的时间比对照组长得多（Ｐ＜０．０５）。提示：猴头菇具有明显的增强运动能力和解除疲劳的作用。     

SRY盒基因在斑马鱼和胡子鲇中的保守性分析

本文采用人类SRY基因探针,与EcoRI消化的中国的胡子鲇(Clarias fusc us)和美国的斑马鱼(Brachydanio rerio)基因组DNA进行了Southern印迹杂交分析。首次在这两种鱼中发现了SRY盒基因及其存在特征;在斑马鱼中发现了明显的限制性片段长度多态性; 并深入分析了SRY盒基因的进化保守性。 Abstract:Using,Southern bloting analysis,EcoRI-digested genomic DNA from Clarias fuscus and Brachydanio rerio were hybridizae to human SRY probe.Hybridization occurred in both male and female of the two species.RFLP associated with SRY-box genes was observed in Brachydanio rerio.The conservation of SRY-box genes was analysed.     

Trace elements in scalp hair of professional drivers and university teachers in Hong Kong

Hair samples of 23 male professional drivers and 20 male university teachers in Hong Kong were collected, and the concentrations of Al, Sb, As, Ca, Cu, Fe, Pb, Mg, Mn, Hg, K, Sr, S, V, and Zn were measured. Both of the target groups fell within the same age group of 35–45. The washing method of using detergent and powder was found to be comparable to that of using ether. Difference in the mean concentration of each detected element in the two groups was tested by the Student'st-test and the Wilcoxon rank-sums test. Hair concentrations of Al, Sb, Pb, Mg, Mn, and K in the «Driver Group» were significantly (p<0.05) higher than those in the “Teacher Group.” On the other hand, As and Hg were found to have a higher concentration in hair of teachers. Interpretation of the findings in terms of the environmental factor and the metabolic rate was attempted.     

Chemical induction of adventitious root formation in Taxus baccata cuttings

The effect of some auxins (IBA and NAA), phenolic compounds (phloroglucinol, gentisic acid and coumarin), a combination of auxins and phenolics, and a systemic fungicide (Bavistin) have been examined for stimulatory effects on adventitious root formation in stem cuttings (current season's growth) of Taxus baccata L. In general lower concentration (0.25 mM) of both IBA and NAA was more effective in inducing rooting of cuttings taken from both male and female trees. The combined treatment of IBA+NAA (0.25 mM each) showed some success in cuttings from male trees only (55%, compared to 15% rooting in cuttings from female trees). Generally, the callus formation was quite high (70%) in all auxin treatments (alone or in combination). Among the phenolics, 40% rooting success was achieved with phloroglucinol only, while coumarin and gentisic acid were ineffective. The combined treatment of auxins and phenolics also failed to promote rooting. On the other hand, Bavistin was extremely effective for callusing (90%) as well as rooting (80%). The effectiveness of various compounds tested for rooting of young stem cuttings declined in the order: 0.25 mM IBA>0.05% Bavistin>0.25 mM NAA>1.25 mM IBA>15 mM phloroglucinol>IBA+NAA (0.25 mM each). In addition to the auxins, IBA and NAA that are widely used for commercial propagation, the auxin-like properties of the fungicide Bavistin could be exploited for adventitious rooting in T. baccata, and in other plant species.     

48例原发性闭经患者的细胞遗传学分析

本文报告对48例原发闭经患者的临床和细胞遣传学分析,共发现染色体异常17例,占35.4%,其中包括45,X,7例;45,X/46,XX,2例;X染色体结构异常5例;核型中有Y染色体3例。讨论了原发闭经的细胞遗传学病因及异常核型与表型的关系。     

耳河螺生殖器官和精子的形态学研究

耳河螺「Ｒｉｖｕｌａｒｉａ ａｕｒｉｃｕｌａｔａ （Ｍａｒｔｅｎｓ）」为雌雄异体。雄性生殖器官由精巢,输精小管,贮精囊,输精管,前列腺和阴茎组成。精巢内有精子,精子有典型精子和非典型精子两种。扫描电镜下,典型精子头部呈螺旋状,尾端只有一根较粗壮;非典型精子头部和中部为棒状,尾部呈扫帚状,由８－１５根鞭毛组成。     

Transformation of Orychophragmus violaceus Using Agrobacterium tumefaciens And Regeneration of Transgenic Plants

ＴｒａｎｓｆｏｒｍａｔｉｏｎｏｆＯｒｙｃｈｏｐｈｒａｇｍｕｓｖｉｏｌａｃｅｕｓＵｓｉｎｇＡｇｒｏｂａｃｔｅｒｉｕｍｔｕｍｅｆａｃｉｅｎｓＡｎｄＲｅｇｅｎｅｒａｔｉｏｎｏｆＴｒａｎｓｇｅｎｉｃＰｌａｎｔｓａ￥ＺＨＯＵＪｉ－ｍｉｎｇ（周冀明）;ＷＥＩＺｈ...