Plant regeneration from callus and explants of Sesbania spp.

Root, hypocotyl and cotyledon explants of Sesbania bispinosa, Sesbania cannabina, Sesbania formosa, and Sesbania sesban were cultured on Murashige and Skoog medium with benzyladenine (BA; 2.22, 4.44, 8.88 M) in combination with 2,4-dichlorophenoxyacetic acid (2,4-d; 2.26, 4.52, 9.05 M), indolebutyric acid (IBA; 0.25, 0.49, 4.92 M) or naphthaleneacetic acid (NAA; 2.69, 5.37, 10.74 M). Although all explant types developed some callus, callus occurred earliest and continued to grow fastest with hypocotyls. Media including 2.4-d or NAA gave the fastest growing callus. Callus was subcultured up to 10 times at 20-day intervals and retained a rapid growth rate. Shoots regenerated readily from both hypocotyls or cotyledons but not from roots. Shoot organogenesis was most frequent with IBA (0.25–4.92 M) in combination with BA (4.44–8.88 M) and did not occur with 2,4-d. With each species at least one medium induced shoot differentiation from more than 50 percent of the callus pieces. With one exception, media containing IBA that induced shoot organogenesis on explants also did so in callus, but media containing NAA, even when effective with explants, did not cause differentiation of callus. Shoots that differentiated were excised and cultured on MS medium without growth regulators or with IBA (2.46, 4.92, 9.84 M). Roots developed after 3–8 days on an appropriate rooting medium, often without IBA. Rooted plantlets were transplanted to pots in a greenhouse and developed into normal plants. Suitable media and protocols for initiating and subculturing callus and regenerating whole plants in vitro from callus and explants have thus been established for four species of Sesbania.     

嗜热蓝藻优雅粘囊藻藻胆体的特性和别藻蓝蛋白的亚基组成

研究了优雅粘囊藻藻胆体（ＰＢＳ）的特性。从聚丙烯酰胺凝胶电泳（ＰＡＧＥ）、吸收光谱和二阶导数图谱可证明,它的ＰＢＳ含有一种红色藻胆蛋白,两种Ｃ－藻蓝蛋白和三种别藻蓝蛋白。但是,用ＰＡＧＥ和羟基磷灰石柱层析分离和纯化所得藻胆蛋白,一般仅得到一种Ｃ－ＰＣ和一种亚基组成特殊的别藻蓝蛋白ＡＰＣ。这种ＡＰＣ吸收光谱和荧光发射相同于已报告的ＡＦＣ６６０ｎｍ。但是它的亚基组成是（αα′ββ′）２而不是（α′α２β２β′）Ｌｃ１０或（αβ）３。     

新疆的刺球座属、穴壳属及普氏腔孢属

本文报道了新疆腔菌纲座囊菌目刺球座属(Lasiobotrys)、穴壳属(Dothiora)和普氏腔孢属(Plowrightia)的六种子囊菌,即:忍冬刺球座菌(L.loniccrae)、花楸穴壳菌 (D.sorbi)及其无性阶段花楸疡壳孢(Dothichiza sorbi)、茶蔗子普氏腔孢菌(P.ribesia)、小檗普氏腔孢菌(P.berberidiJ)、沙棘普氏腔孢菌(P.hippophaeos)及雕刻普氏腔孢菌(P.insculpta)。这三个属的真菌在我国均未报道过,为我国新纪录属(种)。标本均采于新疆,保存于新疆八一农学院植保系真菌标本室(HMAAC)。     

Human TRP-1 Has Tyrosine Hydroxylase but no DOPA Oxidase Activity

Human TRP-1 has been immunopurified from normal human melanocytes cultured from black neonatal subjects and used to investigate the catalytic function of TRP-1 for the two substrates, L-tyrosine and L-DOPA. Immunopurified TRP-1 did not demonstrate DOPA staining on SDS/PAGE nor DOPA oxidase (DO) activity with either routine or modified assays. The purified TRP-1 also demonstrated no tyrosine hydroxylase (TH) activity using the routine Pomerantz assay. However, there was apparent TH activity exhibited by immunopurified TRP-1 under conditions with low tyrosine concentration (≤0.8 μCi/ml of ³H-tyrosine), prolonged incubation time (i.e., overnight) and in the absence of the cofactor L-DOPA. Using these latter specific conditions, TH activity was also detected in cell lysates from a tyrosinase-negative albino melanocyte line which exhibited no TH activity with the routine Pomerantz assay. In addition, TH activity under low substrate assay conditions was not exhibited in a melanocyte line derived from a TRP-1 deficient, Brown albino individual. However, the absence of TH in this Brown albino cell line could be compensated for by the addition of L-DOPA to the assay. These results suggested that TRP-1 has some tyrosine hydroxylase but no DOPA oxidase activity. We propose that one function of TRP-1 is to modulate tyrosinase activity by making DOPA available as a cofactor to perpetuate the initial steps in melanogenesis.     

重组人IL6／2融合蛋白对6.5Gy照射小鼠造血功能恢复的影响

观察了ｈＦＰＩＬ６／２对６．５Ｇｙγ线照射ＮＩＨ小鼠第１０天造血功能恢复的影响。结果表明：照射小鼠连续４ｄ给予ｈＦＰＩＬ６／２２５０μｇ·ｋｇ－１·ｄ－１,其脾重、ＣＦＵ－８、骨髓有核细胞数及ＣＭ－ＣＦＵ分别比对照组增加５９．０％、２７８．５％、５７．９％和１３８．２％,统计学处理均有显著差异;对此四项指标的改善也明显优于２５μｇ组。另外,２５０μｇ剂量组小鼠外周血象３０ｄ的动态观察结果表明,ｈＦＰＩＬ６／２不但能明显提高红细胞和血红蛋白的最低值,而且能使血小板的恢复提前。提示ｈＦＰＩＬ６／２在促进血小板生成和促进红系造血方面可能具有良好的应用前景。     

尼群地平对实验性糖尿病大鼠心肌收缩性的影响

腹腔注射链脲佐霉素（６５ｍｇ／ｋｇ）诱发Ｗｉｓｔａｒ大鼠糖尿病。糖尿病发病４周后,向饲料中加尼群地平（３０ｍｇ·ｋｇ－１·ｄ－１）。结果表明,糖尿病４周时大鼠心室舒张功能首先受损,８周后心室舒张和收缩功能均明显受累。尼群地平处理对糖尿病大鼠的心肌收缩性有一定的改善作用。提示尼群地平对大鼠糖尿病性心肌病有一定有益作用。     

茶叶中总硒含量及其影响因素的研究

茶叶中硒素总量测定结果表明：土壤含硒量的高低是直接影响茶叶中硒的总量。茶树根、茎、叶、果中均有硒元素,叶片是茶硒积累的主要器官,尤其是老叶,其含量是嫩叶的几倍．茶树品种间含量的差异显著,最大差异达10倍以上．毛茶加工的成品茶含硒量受加工技术措施影响较大,其不同等级的含硒量与级别没有线性关系．     

河南木兰属9种植物过氧化物同工酶分析

本文采用聚丙烯酰胺凝胶电泳系统,测定了河南木兰属8种、1变种50多份的成熟叶片材料的过氧化物同工酶酶谱。测定结果表明,该属植物9种、变种的酶谱均有差异性,每种、变种均有特征酶谱,种间酶谱显著大于种内酶谱,根据其酶谱差异性,可以进行生物种、变种的鉴别和良种的选择。为免除其酶带Rf单一指标,鉴别生物种、变种可能出现的问题,作者创立了"酶谱多指标综合判断距离法"分析技术,首次将该属植物种、变种酶谱的酶带数目、Rf、酶带宽度及其活性强弱4个因子的差异性,进行编码联机、电脑运算分析,其结果与该属玉兰亚属内的玉兰派和辛夷派的形态分类相吻合。但是,从酶学观点不支持椭圆叶玉兰作为独立种的存在,以作为河南玉兰的变种为宜,也不支持河南玉兰派和腋花玉兰派的成立,以并入辛夷派为佳。     

烧伤狗浆血游离氨基酸的动态变化

烧伤狗浆血游离氨基酸的动态变化黎君友,周幼勤,赖业馥,赵有,伊少杰ＳｅｑｕｅｎｔｉａｌＣｈａｎｇｅｓｉｎＰｌａｓｍａＦｒｅｅＡｍｉｎｏＡｃｉｄＣｏｎｃｅｎｔｒａｔｉｏｎｉｎＢｕｒｎｅｄＤｏｇｓ￥Ｌｉｊｕｎｙｏｎ;Ｚｈｏｕｙｏｕｑｉｎ;Ｌａｉｙｅｆｕ;...     

林农复合生态系统的原理、特点及其类型

林农复合生态系统是根据生态位、生物种群之间共生、边缘效应、利用层、食物链“加环”和生态系统工程等原理建立起来的。林农复台生态系统依历史发展过程可分为原始林农复合系统、传统林农复会系统和现代林农复合系统;根据生产方式可分为“牧童”式、林农轮作式、林农间作式、食物网链式、树木菜园式、塘雅系统式和复合母系统式等７种类型;依复台种类及其用途来分,主要有林、农系统,林、牧系统,林、渔系统,林、渔、农系统。林、渔、牧系统,林、食用菌系统,林、水生作物、渔系统和林．副系统等８种类型。