贵州剑河寒武系“清虚洞组”管状微体骨骼化石初步研究

华南寒武系地层中广泛富集微体骨骼化石,为解决某些疑难化石的亲缘关系及研究早期后生动物的演化提供了重要化石证据。在贵州剑河八郎"清虚洞组"中发现一些管状微体骨骼化石。经鉴定后主要有4属,分别为小钻孔螺Torellella、似软舌螺Hyolithellus、鞘状螺Coleoloides和表面具鳞片状管状化石Mongolitubulus squamifer。Mongolitubulus分布范围较为广泛,本文结合前人对该化石亲缘关系的探讨及剑河寒武系"清虚洞组"化石的特征,推测M.squamifer可能是高肌虫的装饰刺。     

罗汉松雄球花及其雄配子体发育的形态结构

该研究采用光学显微镜和扫描电镜,观察罗汉松雄球花、小孢子及其配子体发育过程的形态结构特征,以揭示罗汉松小孢子的发生和雄配子体的发育规律,为罗汉松的生殖和杂交组合提供胚胎学证据。结果发现：(1)罗汉松花芽于每年的7月开始分化,至次年5月花粉成熟散粉,雄球花由单生的卵圆形转为2～3个葇荑花序并生,小孢子叶螺旋状着生于圆柱状的花序轴上,每一小孢子叶远轴面基部并列着生2个小孢子囊。(2)小孢子囊壁发育过程中由外及里出现各由1层薄壁细胞组成的表皮、药室内壁、中层和绒毡层,至散粉前,后两者基本被分解吸收。(3)同一小孢子囊内的造孢细胞发育在时间上存在差异,小孢子母细胞减数分裂后形成的四分体有四面体型和十字交叉型两种排列方式,成熟的雄配子体包括生殖细胞和粉管细胞,发育过程中出现的第一和第二原叶细胞大部分被分解消失。(4)电镜下罗汉松花粉粒为典型的松花型花粉,两侧各具1个气囊,远极面具一萌发沟,花粉粒表面具纹理或皱褶。     

矿质营养对生长素代谢影响的研究现状与展望

植物从环境中吸收的矿质元素直接参与植物生理代谢,植物内源激素对植物生理代谢具有重要的调节作用,矿质营养与植物内源激素的相互关系一直是植物营养研究的重要内容。生长素是植物五大内源激素之一,对细胞的分裂和伸长具有重要的调节作用。本文从矿质营养对IAA的量及分布,矿质营养对IAA合成,矿质营养对IAA运输,矿质营养对IAA的转化等方面对矿质营养与生长素的关系的研究进展进行了概述,并对矿质营养与生长素的关系的进一步研究进行了展望。     

阿尔茨海默病核酸疫苗的构建及诱导体液免疫反应的初步研究

近年,内源性蛋白主动免疫预防和治疗阿尔茨海默病(AD)的策略成为AD研究领域的热点,并获得迅猛发展。构建了以β-片层结构的淀粉样蛋白(AB)及其可溶性与病理性突变体为抗原基因的DNA疫苗用于免疫小鼠,经过对免疫方案的探索和调整,结果能够打破其自身耐受,在外周血中诱发出较高滴度的抗-AB抗体。初步探讨了诱导体液免疫学效应的规律。并在原代培养的海马神经元AB毒性模型中验证了免疫后血清的生物学活性。     

小麦高分子量麦谷蛋白亚基分离方法的研究

小麦高分子量麦谷蛋白亚基（ＨＭＷ－ＧＳ）与小麦面包烘烤质量和面粉的加工特性密切相关,ＳＤＳ－ＰＡＧＥ是其常用的分离方法之一。ＳＤＳ－ＰＡＧＥ方法一般分为２类：第一类采用１１％和５％浓度的胶,后者用于分离２亚基和２＾＊亚基,该种方法常使用碱性提取液,需要２次电泳过程,且在５％浓度的胶中ＨＭＷ－ＧＳ易于和麦醇蛋白混淆;另外一类ＳＤＳ－ＰＡＧＥ采用梯度胶,配合使用银染方法,制梯度胶则使用梯度仪及磁力搅拌     

A Comparison of the Biological Characteristics of EV71 C4 Subtypes from Different Epidemic Strains

The comparative analysis of the biological characterization and the genetic background study of EV71 circulating strains is commonly recognized as basic work necessary for development of an effective EV71 vaccine. In this study, we sequenced five EV71 circulating strains, isolated from Fuyang, Hefei, Kunming and Shenzhen city of China and named them FY-23, FY-22, H44, K9 and S1 respectively. The sequence alignment demonstrated their genotypes be C4. The genetic distance of the VP1 gene from these isolates s...     

SOCIAL BEHAVIOUR AND MASS MORTALITY IN THE BASAL CERATOPSIAN DINOSAUR PSITTACOSAURUS (EARLY CRETACEOUS, PEOPLE'S REPUBLIC OF CHINA)

Abstract:  An exceptionally preserved herd of the ceratopsian dinosaur Psittacosaurus was recovered from the Lujiatun Beds of the Lower Cretaceous Yixian Formation, Liaoning Province, north-east China. This specimen provides the earliest direct evidence of post-nestling gregarious behaviour in this important dinosaur clade. The sediment in which the skeletons occur represents a lahar (volcanic mudflow), indicating that the six individuals present were entombed simultaneously during a catastrophic mass mortality event. Reconstruction of the herd age profile shows it was composed of offspring from at least two clutches, revealing that group fidelity in these dinosaurs extended beyond time spent together in the nest. This demonstrates that sociality appeared much earlier in ceratopsian evolution than is generally acknowledged and that such behaviour preceded the evolution of the elaborate horns and frills seen in derived ceratopsids.     

Alterations in sucrose metabolizing enzyme activities and total phenol content of Curcuma Ionga L. as affected by different triazole compounds

Changes in the sucrose metabolism of Cur-cuma longa L. plants were studied under treatment with different triazole compounds viz., triadimefon (TDM) and propiconazole (PCZ). Plants were treated with TDM at 15 mg/L and PCZ at 10 mg/L separately by soil drenching on 80, 110, and 140 days after planting (DAP). The plants were harvested randomly on 90, 120, and 150DAP to determine the effect of both the triazoles on sucrose metabolizing enzymes and phenol content. The sucrose metabolism was studied by analyzing sucrose metaboliz-ing enzymes like sucrose synthase and sucrose phosphate synthase. All the analyses were assayed in leaves and tubers of both control and treated plants. It was found that both of the triazole compounds had profound effects on these parameters.     

去甲基化药物治疗肿瘤新进展

人类肿瘤的发生是多基因、多步骤共同作用的结果,抑癌基因在这一进程中扮演着重要的角色。最新研究表明,在肿瘤形成过程中,启动子区异常高甲基化的抑癌基因（包括几个关键基因）始终保持沉默、功能丧失,而启动子区去甲基化后抑癌基因则可以重新激活,其产物恢复表达。据此,人们开发出了去甲基化药物,并已应用于临床治疗及研究,结果证实去甲基化药物是一类安全、有效的抗肿瘤新药。随着研究的不断深入,必将有更多的去甲基化药物问世。     

Estimation of Cellobiohydrolase I Activity by Numerical Differentiation of Dynamic Ultraviolet Spectroscopy

1,4-β-D-glucan cellobiohydrolase I (CBH I),p-nitrophenyl β-D-cellobioside,p-nitrophenol andcellobiose show distinct ultraviolet spectra,allowing the design of an assay to track the dynamic process ofp-nitrophenyl β-D-cellobioside hydrolysis by CBH I.Based on the linear relationship between p-nitrophenolformation in the hydrolysate and its first derivative absorption Curve of AUC340_400_(nm)(area under the curve),a new sensitive assay for the determination of CBH I activity was developed.The dynamic parameters ofcatalysis reaction,such as Vm and k_(cat),can all be derived from this result.The influence of β-glucosidase andendoglucanase in crude enzyme sample on the assay was discussed in detail.This approach is useful foraccurate determination of the activity of CBHs.