人乳腺癌cAMP结合蛋白

建立了测定人乳腺癌胞浆ｃＡＭＰ结合蛋白（ｃＡＭＰｂ．ｐ．）方法。综合研究了其温度、保温时间、配体浓度、稳定性等条件。ｃＡＭＰｂ．ｐ．的Ｋ_Ｄ值为２．９０×１０~（－８）ｍｏｌ／Ｌ．并测定了６０例雌激素受体（ＥＲ）Ｆｕ性乳腺癌标本的ｃＡＭＰｂ．ｐ．含量。此组病人术后均接受系统的内分泌治疗,ＥＲ／ｃＡＭＰｂｐ,比值范围为７．７～３６２×１０~（－３）,ＥＲ／ｃＡＭＰｂ．ｐ．比值≥４０×１０~（－３）的五年生存率明显高于比值＜４０×１０~（－３）组,（ｐ＜０．００５）．表明测定ＥＲ／ｃＡＭＰｂ．ｐ．比值对预测患者内分泌治疗疗效,优于单独测定ＥＲ．     

Characterization of a Chlamydomonas reinhardtii gene encoding a protein of the DNA photolyase/blue light photoreceptor family

The organization and nucleotide sequence of a gene from Chlamydomonas reinhardtii encoding a member of the DNA photolyase/blue light photoreceptor protein family is reported. A region of over 7 kb encompassing the gene was sequenced. Northern analysis detected a single 4.2 kb mRNA. The gene consists of eight exons and seven introns, and encodes a predicted protein of 867 amino acids. The first 500 amino acids exhibit significant homology with previously sequenced DNA photolyases, showing the closest relationship to mustard (Sinapis alba) photolyase (43% identity). An even higher identity, 49%, is obtained when the Chlamydomonas gene product is compared to the putative blue-light photoreceptor (HY4) from Arabidopsis thaliana. Both the Chlamydomonas and the Arabidopsis proteins differ from the well characterized DNA photolyases in that they contain a carboxyl terminal extension of 367 and 181 amino acids, respectively. However, there is very little homology between the carboxyl terminal domains of the two proteins. A previously isolated Chlamydomonas mutant, phrl, which is deficient in DNA photolyase activity, especially in the nucleus, was shown by RFLP analysis not to be linked to the gene we have isolated. We propose this gene encodes a candidate Chlamydomonas blue light photoreceptor.     

绞股蓝属的染色体研究

报道了葫芦科绞股蓝属（Ｇｙｎｏｓｔｅｍｍａ Ｂ１．）８种共２０个居群的染色体数目,分别为２ｎ＝２２,３３,４４,６６,８８多倍体现象极为普遍。两个亚属;绞股蓝亚属（Ｓｕｂｇｅｎ．Ｇｙｎｏｓｔｅｍｍａ）和喙果藤亚属（Ｓｕｂｇｅｎ．Ｔｒｉｏｓｔｅｌｌｕｍ）的染色体基数均为Ｘ＝１１,并结合该属植物形态特征、繁殖方式和地理分布,对普遍出现的多倍体现象进行了讨论。     

Cloning of cDNA for Vitellogenin of the Parasitoid Wasp, Pimpla nipponica (Hymenoptera: Apocrita: Ichneumonidae): Vitellogenin Primary Structure and Evolutionary Considerations

The cDNA for vitellogenin (Vg) of the parasitoid wasp Pimpla nipponica (Hymenoptera: Apocrita) was cloned and sequenced.¹ The deduced amino acid sequence with 1807 residues was obtained. The N-terminal 20 amino acids chemically determined for vitellin (Vn) agreed completely with the deduced 20 amino acids that follow the 16 amino acid residues for putative signal peptide. The cDNA clone for the Vg of the turnip sawfly Athalia rosae (Hymenoptera: Symphyta), previously obtained and partially sequenced, was also completely sequenced and the amino acid sequence deduced. Amino acid sequences were compared between these two species and also with known Vg sequences from other insects. Common to all these insects is the presence of two long regions with relatively well-conserved amino acid sequences, one near the N-terminal extending 267–282 residues (including two cysteines at conserved locations), and the other starting at position 450 to 655 and extending 279–283 residues, and of a region at the C-terminal extending some 200 residues (about 250 in Aedes aegypti due to the presence of a serine-rich stretch) with 10 cysteines at conserved locations. A molecular phylogenetic tree was constructed.     

藻胆蛋白复合物的合成及其分子内能量传递

通过偶联剂3-(2-吡啶联巯基)丙酸N-羟基琥珀亚胺酯(SPDP)及改变配料比, 合成了两个R-藻红蛋白(R-PE)与C-藻蓝蛋白(C-PC)的复合物A和B.利用吸收光谱确定了分子内R-PE与C-PC的摩尔比为6∶1和2∶1. 通过荧光光谱, 观察到能量传递现象, 并计算出能量传递效率为63%和88%.证明分子内能量传递效率很高. 二硫苏糖醇(DTT)还原连接R-PE与C-PC的二硫桥键后, 能量传递被阻断. 这一现象进一步证明复合物中存在分子内能量传递.     

Studying dynamic process of photosensitization by ESR method

By kinetic methods, functional relation of TAN radical, produced in the process of TEMPONE trapping O2, vs. time during photosensitization was established. Accordingly relative rate constants of generating all kinds of active intermediates through types I and II mechanism of photosensitization can be calculated. Using the formula and experimental results, the relative rate constants of generating O2, O2 and PS2 of three kinds of perylenequinone photosensitizer: HA, HB and CP in DMF-H20 and DMSO-H2O system were calculated, and then the structure-activity relationship of perylenequinone photosensitizer and the relation between photosensitivity and solvent was studied.     

中华大蟾蜍输卵管促受精因子的实验分析

在孕酮作用厂,切除卵巢的中华大蟾蜍输卵管上皮细胞不仅能够使外源移植的卵母细胞在输卵管中正常运行,还保证卵母细胞正常受精。根据输卵管卷曲部分泌物的ＳＤＳ－ＰＡＧＥ分析,存在依赖孕酮的３３ｋＤ蛋白质。经兔抗３３ｋＤ蛋白抗体处理具输卵管分泌物的卵母细胞,受精率受到显著抑制·提示该蛋白有促受精活性。输卵管分泌物介入受精涉及复杂的多种因子。     

高必需氨基酸转基因马铃薯的研究

８０年代以来,马铃薯遗传转化系统日趋成熟,转基因工程植株已被广泛应用于基础科学研究［１］。作为食物蛋白和能量主要来源的马铃薯,提高其蛋白质含量及质量的遗传工程研究正受到人们的普遍关注［２］。Ｙａｎｇ等［２］将旨在改善氨基酸平衡的ＣＡＴ－ＨＥＡＡＥ（氯酶素乙酰转移酶－高含量人体必需氨基酸）融合基因导入马铃薯,获得了Ｓｏｕｔｈｅｒｎｂｌｏｔ、Ｎｏｒｔｈｅｒｎｂｌｏｔ、Ｗｅｓｔｅｒｎｂｌｏｔ的证据,但尚缺少氨基酸分析的资料。玉米醇溶蛋白（ｚｅｉｎ）［３］是一个富含甲硫氨酸的贮存蛋白,它和人工合成的ＨＥ…     

干旱和湿润生境下辽东栎群体遗传结构及其适应意义的初步研究

应用同工酶分析方法,测定北京市东灵山区两个分别代表干旱和湿润生境的辽东栋（ＱｕｅｒｃｕｓｌｉａｏｔｕｎｇｅｎｓｉｓＫｏｉｚ．）群体的遗传结构。共分析统计了１３ 个酶系统３０ 个位点。结果表明：辽东栎群体内部存在丰富的遗传变异（多态位点百分率为８６．６％ ,等位基因平均数为２．２５）。两群体遗传结构的相似性程度很高（Ｄ＝ ０．０２９, ＧＳＴ＝ ０．０４８）;但在个别位点上仍存在较大差异,这些差异的产生可能与对小生境的适应有关。对不同年龄段的初步分析结果显示,基因频率的动态变化可能有其适应意义     

杨树新品种叶肉原生质体培养和植株再生

从１ 个月龄的ＮＬ－８０１０６ 杨（Ｐｏｐｕｌｕｓｄｅｌｔｏｉｄｅｓ×Ｐ． ｓｉｍ ｏｎｉｉ）无菌苗叶片分离得到大量原生质体,纯化后其原生质体产量为４×１０７／ｇ ｆｒ．ｗ ｔ． 纯化的原生质体在含２,４－Ｄ ２ ｍ ｇ／Ｌ、ＮＡＡ ０．５ ｍ ｇ／Ｌ和ＫＴ ０．５ ｍ ｇ／Ｌ的ＫＭ８ｐ 和ＭＳ培养基中进行高密度液体浅层培养,渗透势为０．４０ ｍ ｏｌ／Ｌ的ＫＭ８ｐ 培养基中原生质体分裂频率最高． 培养第５ 天观察到第一次细胞分裂,培养１０ ｄ 的分裂频率为４．５％ ,１２ 周内可形成大量的细胞团和小愈伤组织． ＮＬ－８０１０６杨叶肉原生质体在富含有机氮并以葡萄糖为碳源的培养基中具有较高的分裂频率和植板率．小愈伤组织在ｇｅｌｒｉｔｅ 固化的ＮＬＺ１ 培养基上增殖生长,３ 周后形成４—６ ｍ ｍ 结构紧密的鲜红色愈伤组织,转至ＮＬＦ分化培养基,分化成苗率为１００％ ． 待芽伸长到３ ｃｍ 时,从基部切下转至１／２ ＭＳ培养基上诱导生根,形成完整植株