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991.
Jing Lu Mingxin Wen Yurong Huang Xiuquan He Yunshan Wang Qi Wu Zengchun Li Andres Castellanos-Martin Mar Abad Juan J. Cruz-Hernandez Cesar A. Rodriguez Jesus Perez-Losada Jian-Hua Mao Guangwei Wei 《Epigenetics》2013,8(6):571-583
Recently, it has been suggested that C2ORF40 is a candidate tumor suppressor gene in breast cancer. However, the mechanism for reduced expression of C2ORF40 and its functional role in breast cancers remain unclear. Here we show that C2ORF40 is frequently silenced in human primary breast cancers and cell lines through promoter hypermethylation. C2ORF40 mRNA level is significantly associated with patient disease-free survival and distant cancer metastasis. Overexpression of C2ORF40 inhibits breast cancer cell proliferation, migration and invasion. By contrast, silencing C2ORF40 expression promotes these biological phenotypes. Bioinformatics and FACS analysis reveal C2ORF40 functions at G2/M phase by downregulation of mitotic genes expression, including UBE2C. Our results suggest that C2ORF40 acts as a tumor suppressor gene in breast cancer pathogenesis and progression and is a candidate prognostic marker for this disease. 相似文献
992.
Hai Chun Liu Hua Zhao Jian Chen Wen Liang Wu Hong Liang Wang Gang Jun Jiao Yun Zhen Chen 《Journal of molecular histology》2013,44(5):535-544
Decreased levels of serum insulin-like growth factor-1 (IGF-1) have been proven to cause osteoporosis. Gene transfer of IGF-1 offers an attractive technology to treat skeletal metabolic disorders including osteoporosis, but the viral vectors are limited by their high antigenicity and immune response. Our purpose was to investigate the expression of a non-invasive vector, recombinant plasmid enhanced green fluorescent protein-N1 (pEGFP-N1) that transferred IGF-1 gene into ovariectomized (OVX) rats in vivo and evaluate the effect of this therapy on osteoporosis. OVX or sham operations were performed in 60 female, 7-month-old unmated SD rats. 12 weeks after OVX operation, the vectors were transfected to the 10-month-old rats and experimental data were detected from 48 h to 7 week after transfection. Our results showed that remarkable expression of fluorescence and serum IGF-1 was observed in the rats transfected by recombinant plasmids, indicating that IGF-1 gene was successfully transferred to OVX rats by injecting the vector through hydrodynamic method via the tail vein. The bone metabolism index including serum alkaline phosphatase, the histomorphometric parameters of lumbar vertebra including trabecular area percentage, trabecular thickness, trabecular number and trabecular separation, and the bone mineral density (BMD) and biomechanical parameters of lumbar vertebra including BMD, maximum condensing force, crushing strength in OVX rats transfected by pEGFP-N1-IGF-1 were improved remarkably compared with OVX+pEGFP-N1 rats, indicating that the transfection of recombinant plasmid pEGFP-N1-IGF-1 played a significant role in alleviating osteoporosis in rats induced by OVX. This encouraged a potential approach of IGF-1 gene therapy to the treatment of osteoporosis. 相似文献
993.
Caiyong Chen Daniel Garcia-Santos Yuichi Ishikawa Alexandra Seguin Liangtao Li Katherine H. Fegan Gordon J. Hildick-Smith Dhvanit I. Shah Jeffrey D. Cooney Wen Chen Matthew J. King Yvette Y. Yien Iman J. Schultz Heidi Anderson Arthur J. Dalton Matthew L. Freedman Paul D. Kingsley James Palis Barry H. Paw 《Cell metabolism》2013,17(3):343-352
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995.
Wen Fong Ooi Catherine Ong Tannistha Nandi Jason F. Kreisberg Hui Hoon Chua Guangwen Sun Yahua Chen Claudia Mueller Laura Conejero Majid Eshaghi Roy Moh Lik Ang Jianhua Liu Bruno W. Sobral Sunee Korbsrisate Yunn Hwen Gan Richard W. Titball Gregory J. Bancroft Eric Valade Patrick Tan 《PLoS genetics》2013,9(9)
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Highly sensitive and selective resonance Rayleigh scattering (RRS) and surface enhanced resonance Raman scattering (SERRS) spectral detection technique are developed by combining the functional nucleic acid including aptamer and DNAzyme, and nanoparticle such as gold/silver (NG/NS) aggregation and catalysis reaction. The recent progress of resonance scattering spectral technologies including RRS and SERRS are reviewed in this paper. 相似文献
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