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171.
排卵,排精前后文昌鱼体内GnRH的研究 总被引:1,自引:1,他引:0
利用放射免疫分析法测定了排卵、排精前后青岛文昌鱼体内促性腺激素释放激素(GnRH)的含量变化,并通过高效液相色谱比较了雌、雄文昌鱼性腺及除性腺外体部GnRH的种类和含量的异同。结果表明:1)生殖过程中雌、雄文昌鱼体内GnRH含量的变化规律不同;雌性文昌鱼体内GnRH总含量在排卵时有所下降,排后12小时政策最为明显,以后逐渐上升到排前水平;雄性文昌鱼仅在排精时有所下降,2小时后即稳定在排水平。2)文 相似文献
172.
Chronic Nicotine Administration Differentially Affects Neurotransmitter Release from Rat Striatal Slices 总被引:6,自引:3,他引:3
Abstract: The objective of these experiments was to determine whether the chronic administration of nicotine, at a dose regimen that increases the density of nicotine binding sites, alters the nicotine-induced release of [3H]dopamine ([3H]DA), [3H]norepinephrine ([3H]NE), [3H]serotonin ([3H]5-HT), or [3H]acetylcholine ([3H]ACh) from rat striatal slices. For these experiments, rats received subcutaneous injections of either saline or nicotine bitartrate [1.76 mg (3.6 µmol)/kg, dissolved in saline] twice daily for 10 days, and neurotransmitter release was measured following preloading of the tissues with [3H]DA, [3H]NE, [3H]5-HT, or [3H]choline. Chronic nicotine administration did not affect the accumulation of tritium by striatal slices, the basal release of radioactivity, or the 25 mM KCl-evoked release of neurotransmitter. Superfusion of striatal slices with 1, 10, and 100 µM nicotine increased [3H]DA release in a concentration-dependent manner, and release from slices from nicotine-injected animals was significantly (p < 0.05) greater than release from saline-injected controls; release from the former increased to 132, 191, and 172% of release from the controls following superfusion with 1, 10, and 100 µM nicotine, respectively. Similarly, [3H]5-HT release increased in a concentration-related manner following superfusion with nicotine, and release from slices from nicotine-injected rats was significantly (p < 0.05) greater than that from controls. [3H]5-HT release from slices from nicotine-injected rats evoked by superfusion with 1 and 10 µM nicotine increased to 453 and 217%, respectively, of release from slices from saline-injected animals. The nicotine-induced release of [3H]NE from striatal slices was also concentration dependent but was unaffected by chronic nicotine administration. [3H]ACh release from striatal slices could not be detected when samples were superfused with nicotine but was measurable when tissues were incubated with nicotine. The release of [3H]ACh from slices from nicotine-injected rats was significantly (p < 0.05) less than release from controls and decreased to 36, 83, and 77% of control values following incubation with 1, 10, or 100 µM nicotine, respectively. This decreased [3H]ACh release could not be attributed to methodological differences because slices from nicotine-injected rats incubated with nicotine exhibited an increased [3H]DA release, similar to results from superfusion studies. In addition, it is unlikely that the decreased release of [3H]ACh from striatal slices from nicotine-injected rats was secondary to increased DA release because [3H]ACh release from slices from hippocampus, which is not tonically inhibited by DA, also decreased significantly (p < 0.05) in response to nicotine; hippocampal slices from nicotine-injected rats incubated with 1 and 10 µM nicotine decreased to 42 and 70%, respectively, of release from slices from saline-injected animals. Results indicate that the chronic administration of nicotine increases the ability of nicotine to induce the release of [3H]DA and [3H]5-HT and decreases the ability of nicotine to evoke the release of [3H]ACh but does not alter the nicotine-induced release of [3H]NE from brain slices. 相似文献
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174.
大鼠心脏血压超负荷诱导左心室HSP70基因表达 总被引:15,自引:1,他引:14
本工作应用热休克蛋白70(HSP70)核酸分子杂交方法,测定了大鼠腹主动脉缩窄后左心室HSP70mRNA的水平。结果表明:大鼠腹主动脉缩窄后4h,动脉血压已明显升高,并持续在高水平上;大鼠左心室重/体重比在第三天开始增加,然后持续升高,第4周时比对照组增加59%;左心室HSP70mRNA在腹主动脉缩窄后4h已明显升高,1d,2d,1w均维持在高水平,1w后逐渐消失。实验结果提示:大鼠心肌负荷增加早期,左心室HSP70mRNA表达明显增加。 相似文献
175.
本研究报道从睡眠剥夺(SD)48—72h的灵长类原宗(primitivestock)Tupaiabelangerichinensis(TBC)提取内源性“睡眠因子”(sleepfactor)S2C和S4B。收集的尿液经超滤,清液冻干经SephadexG15分离得到FractionⅠ-Ⅴ。活性测定发现Fraction-Ⅲ(S2C)呈现显著δ-增强促眠效应。经SephadexG25和SephadexLH20进一步净化的S4B也呈现显著δ-增强促眠效应。 相似文献
176.
不同生境下蓝桉的木材解剖研究 总被引:9,自引:0,他引:9
用光镜及扫描电镜对不同生境下的蓝桉(Eucalyptusglobulus Labill.)木材结构进行了解剖学观察,描述了木材结构特征。以蓝桉木材的11 个主要特征——导管频率、单孔率、导管直径、导管分子长度、管胞长度和直径、纤维管胞长度和直径、纤维长度、射线频率和高度为指标,对生长在不同生境下的蓝桉进行比较发现:随着纬度的增加,生长轮变化不明显;木材中大部分组成分子的数量特征,除导管频率有降低趋势外均与纬度成正相关。评述了年降雨量对蓝桉木材结构的影响 相似文献
177.
178.
179.
芦苇耐盐变异植株及其细胞学鉴定 总被引:5,自引:0,他引:5
用甲基磺酸乙酯(EMS)处理芦苇(Phragm itescom m unis Trin.)胚性愈伤组织。从处理后的愈伤组织诱导获得芦苇耐盐变异植株R5002-12。变异植株能在含有1% NaCl的MS培养基上生长。细胞学检查变异植株是混倍体,染色体数目变异范围在100至33 之间。分蘖植株具有相似的形态学及染色体变异特性 相似文献
180.
The interfering effects of copper, zinc, and cobalt on the uptake of mugineic acid-ferric complex were studied in barley ( Hordeum vulgare , cv. Minorimugi) grown in nutrient solution. Short-term uptake experiments of 3 h were performed utilizing both ionic and mugineic acid-complex forms of each metal at two different concentrations. Copper was most effective in decreasing iron uptake when added in an ionic form at either concentration. The inhibition order at higher concentrations followed Cu(II) > Zn(II) ≥ Co(II), Co(III), which is consistent with the stability constants of these metal complexes with mugineic acid. The displacement of iron from its mugineic acid complex by these metals is suggested as a probable explanation for the decreased iron uptake. The inhibitory effect of metal complexes with mugineic acid on iron uptake was only found in cases with higher concentrations of Cu(II) and Zn(II) complexes. Deformation of the specific iron transport system in the plasma membrane due to their adsorption may be responsible for this effect. 相似文献