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931.
932.
Tang TT Zhu ZF Wang J Zhang WC Tu X Xiao H Du XL Xia JH Dong NG Su W Xia N Yan XX Nie SF Liu J Zhou SF Yao R Xie JJ Jevallee H Wang X Liao MY Shi GP Fu M Liao YH Cheng X 《PloS one》2011,6(9):e24272
Objective
Animal studies suggest that regulatory T (Treg) cells play a beneficial role in ventricular remodeling and our previous data have demonstrated defects of Treg cells in patients with chronic heart failure (CHF). However, the mechanisms behind Treg-cell defects remained unknown. We here sought to elucidate the mechanism of Treg-cell defects in CHF patients.Methods and Results
We performed flow cytometry analysis and demonstrated reduced numbers of peripheral blood CD4+CD25+FOXP3+CD45RO−CD45RA+ naïve Treg (nTreg) cells and CD4+CD25+FOXP3+CD45RO+CD45RA− memory Treg (mTreg) cells in CHF patients as compared with non-CHF controls. Moreover, the nTreg/mTreg ratio (p<0.01), CD4+CD25+FOXP3+CD45RO− CD45RA+CD31+ recent thymic emigrant Treg cell (RTE-Treg) frequency (p<0.01), and T-cell receptor excision circle levels in Treg cells (p<0.01) were lower in CHF patients than in non-CHF controls. Combined annexin-V and 7-AAD staining showed that peripheral Treg cells from CHF patients exhibited increased spontaneous apoptosis and were more prone to interleukin (IL)-2 deprivation- and CD95 ligand-mediated apoptosis than those from non-CHF individuals. Furthermore, analyses by both flow cytometry and real-time polymerase chain reaction showed that Treg-cell frequency in the mediastinal lymph nodes or Foxp3 expression in hearts of CHF patients was no higher than that of the non-CHF controls.Conclusion
Our data suggested that the Treg-cell defects of CHF patients were likely caused by decreased thymic output of nascent Treg cells and increased susceptibility to apoptosis in the periphery. 相似文献933.
Enrichment of a common wheat genetic map and QTL mapping for fatty acid content in grain 总被引:2,自引:0,他引:2
Ying-ying Wang Xian-yin Sun Yan Zhao Fan-mei Kong Ying Guo Gui-zhi Zhang Yan-yan Pu Ke WuSi-shen Li 《Plant science》2011,181(1):65-75
DArT and SSR markers were used to saturate and improve a previous genetic map of RILs derived from the cross Chuan35050 × Shannong483. The new map comprised 719 loci, 561 of which were located on specific chromosomes, giving a total map length of 4008.4 cM; the rest 158 loci were mapped to the most likely intervals. The average chromosome length was 190.9 cM and the marker density was 7.15 cM per marker interval. Among the 719 loci, the majority of marker loci were DArTs (361); the rest included 170 SSRs, 100 EST-SSRs, and 88 other molecular and biochemical loci. QTL mapping for fatty acid content in wheat grain was conducted in this study. Forty QTLs were detected in different environments, with single QTL explaining 3.6-58.1% of the phenotypic variations. These QTLs were distributed on 16 chromosomes. Twenty-two QTLs showed positive additive effects, with Chuan35050 increasing the QTL effects, whereas 18 QTLs were negative with increasing effects from Shannong483. Six sets of co-located QTLs for different traits occurred on chromosomes 1B, 1D, 2D, 5D, and 6B. 相似文献
934.
The common wheat line three-pistil (TP) is a valuable mutant for wheat breeding. The TP mutation has normal spike morphology; however, it only produces three pistils per floret. Therefore, it has potential to increase the grain number per spike. In order to determine the underlying molecular mechanism, an annealing control primer system was used to identify the different expressed genes in three-pistil mutation. Using 120 arbitrary ACP primers, we identified three differentially expressed genes in young spikes between two near-isogenic lines (i.e., Chuanmai 28 TP and Chinese Spring TP) and their recurrent parents. We tentatively designated the three differentially expressed genes as DETP-1, DETP-2, and DETP-3. DETP-1 showed similar function with maize cytoplasmic membrane protein, which is involved in cell division in bacteria. DETP-3 is homologous to maize endo-1, 4-beta-glucanase (EGases), which is associated with plant development, cell wall loosening, stem flowering, and root expansion. DETP-2 showed no significant hit with any sequence found in the database and translates unknown protein. These genes would likely play an important role in determining the three pistils trait in wheat. 相似文献
935.
间作缓解蚕豆连作障碍的根际微生态效应 总被引:7,自引:0,他引:7
通过田间小区试验,研究了3个品种蚕豆(92-24、云豆324、凤豆6号)与小麦间作对蚕豆产量、枯萎病病情指数、根际镰刀菌数量、根际真菌代谢功能多样性和土壤酶活性的影响。结果表明:与单作蚕豆相比,云豆324与小麦间作(YD324/W)和凤豆6号与小麦间作(FD6/W)处理均显著提高了蚕豆地上部干重、籽粒产量和百粒重。YD324/W和FD6/W处理使蚕豆枯萎病发病初期病情指数分别降低57.14%和41.67%,镰刀菌数量分别降低32.06%和29.88%,而92-24与小麦间作(92-24/W)处理蚕豆产量、枯萎病病情指数和镰刀菌数量与单作蚕豆均无显著差异。YD324/W和FD6/W处理显著提高了蚕豆根际真菌的多样性指数和丰富度指数,并使蚕豆根际真菌的AWCD值分别比单作蚕豆提高了61.75%和46.49%;YD324/W和FD6/W处理明显改变了蚕豆根际真菌的群落结构。而92-24/W处理对蚕豆根际真菌的多样性指数、丰富度指数和AWCD值均无显著影响,也未明显改变真菌的群落结构。不同发病时期,YD324/W和FD6/W处理均显著提高了蚕豆根际土壤的蔗糖酶、脲酶和过氧化氢酶活性;而92-24/W处理蚕豆根际蔗糖酶、脲酶和过氧化氢酶活性与单作蚕豆均无显著差异。总之,小麦与不同品种蚕豆间作改变了蚕豆根际的真菌群落结构,提高了蚕豆根际真菌的活性、多样性和丰富度,提高土壤酶活性并改善蚕豆生长,增加了蚕豆产量。表明小麦与蚕豆间作改善了根际土壤的微生态环境,降低了镰刀菌的数量,缓解了蚕豆连作障碍,但蚕豆品种的差异影响间作控病效果。 相似文献
936.
【背景】广聚萤叶甲是恶性入侵杂草豚草的一种重要专一性天敌,在冬季低温且缺乏食物的条件下,广聚萤叶甲的耐饥饿能力直接关系到其越冬种群的虫源基数。【方法】在室内观察广聚萤叶甲成虫在10℃低温条件下的存活率和死亡率,研究了水分(有水、无水)、不同密度(一雌一雄、二雌二雄、五雌五雄)、加入泥土枯枝对广聚萤叶甲耐饥饿能力的影响。【结果】无水、有水条件下,广聚萤叶甲成虫的平均存活时间分别为(15.23±1.01)、(13.33±0.88)d,水分对广聚萤叶甲的耐饥力的影响不显著;随着密度的增加,广聚萤叶甲的耐饥饿能力增强,一雌一雄、二雌二雄和五雌五雄的平均存活时间依次为(11.96±0.57)、(13.78±0.60)、(14.81±0.42)d;加入泥土和豚草枯枝后,广聚萤叶甲的耐饥饿能力明显提高,其平均存活时间为(15.97±1.05)d。【结论与意义】低温条件下广聚萤叶甲的耐饥饿能力较强,可保证部分广聚萤叶甲的自然种群在野外安全越冬,研究低温条件下广聚萤叶甲的耐饥饿能力对豚草的生物防治具有重要意义。 相似文献
937.
武汉东湖浮游病毒的丰度及多样性 总被引:5,自引:0,他引:5
运用透射电镜及荧光显微技术 ,对富营养化的武汉东湖中浮游病毒丰度及多样性进行了研究。电镜观察和计数结果显示 ,东湖中浮游病毒的丰度达到 10 8个 /mL。荧光显微镜计数结果约是电镜计数结果的 2 72倍 ,差异极显著 (P <0 0 1)。在东湖超富营养区的水样中观察到了多种与各种病毒形态类似的颗粒 ,其中大部分与噬菌体和噬藻体类似 ,具多种形态的尾部和六边形头部。还有一些线状、杆状、子弹形等形态的病毒粒子。研究结果显示东湖水环境中的浮游病毒不仅丰度极高 ,而且种类丰富。提示浮游病毒在东湖水环境和水生态系统中可能扮演重要角色。 相似文献
938.
为建立人低密度脂蛋白受体(LDLR)、CD81和浸入诱导蛋白L(Sip—L)等多个HCV感染相关分子共表达的转基因小鼠,首先分别构建了白蛋白启动子调控的人LDLR、CD81和Sip-L基因的小鼠肝脏组织特异性表达载体,将3种质粒同时采用水动力转染技术导入小鼠体内,FIT—PCR和免疫组化技术检测转入体内基因的表达及持续时间。结果表明,转染8h后即可检测到3种基因在体内转录,人LDLR和CD81在转染1~4d后可在50%-90%的肝细胞中高效表达,7d后检测不到目的基因表达。为了进一步延长转染基因在小鼠体内的表达时间,又分别构建了人LDLR、CD81和Sip—L的染色体整合型表达载体,将它们与噬菌体ФC31的整合酶表达载体同时水动力转染小鼠,3种基因在体内表达时间可持续到转染后25d。以上结果表明建立了多个HCV感染相关分子共表达的转基因小鼠,为确定这些分子能否使小鼠感染HCV奠定了基础。 相似文献
939.
Genetic analysis and characterization of a new maize association mapping panel for quantitative trait loci dissection 总被引:3,自引:0,他引:3
Xiaohong Yang Jianbing Yan Trushar Shah Marilyn L. Warburton Qing Li Lin Li Yufeng Gao Yuchao Chai Zhiyuan Fu Yi Zhou Shutu Xu Guanghong Bai Yijiang Meng Yanping Zheng Jiansheng Li 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(3):417-431
Association mapping based on the linkage disequilibrium provides a promising tool to identify genes responsible for quantitative variations underlying complex traits. Presented here is a maize association mapping panel consisting of 155 inbred lines with mainly temperate germplasm, which was phenotyped for 34 traits and genotyped using 82 SSRs and 1,536 SNPs. Abundant phenotypic and genetic diversities were observed within the panel based on the phenotypic and genotypic analysis. A model-based analysis using 82 SSRs assigned all inbred lines to two groups with eight subgroups. The relative kinship matrix was calculated using 884 SNPs with minor allele frequency ≥20% indicating that no or weak relationships were identified for most individual pairs. Three traits (total tocopherol content in maize kernel, plant height and kernel length) and 1,414 SNPs with missing data <20% were used to evaluate the performance of four models for association mapping analysis. For all traits, the model controlling relative kinship (K) performed better than the model controlling population structure (Q), and similarly to the model controlling both population structure and relative kinship (Q + K) in this panel. Our results suggest this maize panel can be used for association mapping analysis targeting multiple agronomic and quality traits with optimal association model. 相似文献
940.
Gabriel O. Reznik Prashanth Vishwanath Michelle A. Pynn Joy M. Sitnik Jeffrey J. Todd Jun Wu Yan Jiang Brendan G. Keenan Andrew B. Castle Richard F. Haskell Temple F. Smith Ponisseril Somasundaran Kevin A. Jarrell 《Applied microbiology and biotechnology》2010,86(5):1387-1397
Surfactants find wide commercial use as foaming agents, emulsifiers, and dispersants. Currently, surfactants are produced from petroleum, or from seed oils such as palm or coconut oil. Due to concerns with CO2 emissions and the need to protect rainforests, there is a growing necessity to manufacture these chemicals using sustainable resources In this report, we describe the engineering of a native nonribosomal peptide synthetase pathway (i.e., surfactin synthetase), to generate a Bacillus strain that synthesizes a highly water-soluble acyl amino acid surfactant, rather than the water insoluble lipopeptide surfactin. This novel product has a lower CMC and higher water solubility than myristoyl glutamate, a commercial surfactant. This surfactant is produced by fermentation of cellulosic carbohydrate as feedstock. This method of surfactant production provides an approach to sustainable manufacturing of new surfactants. 相似文献