Causes and Consequences of Changes in Nutrient Structure in the Jiaozhou Bay

Concentrations and ratios of nutrients in Jiaozhou Bay, China, have changed much in the past decades, with trends indicating an increase in nitrogen and a decrease in silicate. Statistical analysis has shown that the long-term variations of nutrients are associated with agricultural activities, precipitation, and anthropogenic factors. Stoichiometric calculations indicate that the nutrient structure has become more and more unbalanced. There has been almost no possibility for nitrogen limitation since the 1980s, the probability of P limitation has increased, and the probability of Si limitation has also increased markedly from the 1980s to the 1990s. As a consequence of changes in nutrient structure, a decrease in the abundance of net phytoplankton was evident, whereas total chlorophyll a levels have remained roughly unchanged at around 3.55 μg/L. Thus, it is likely that smaller species have taken the niche vacated by the larger species. Changes in phytoplankton size and species composition may ultimately lead to various functional and structural changes at the system level.     

黑色素皮质素受体激动剂的高通量筛选模型研究

为了建立黑色素皮质素受体（MC4R）激动剂的高通量筛选方法,将人的MC4R基因质粒（hMC4R/pCDNA3.1）与报告基因质粒（3×CRE/3×MRE/SRE-LUC）按1∶5的比例共转染到HEK293细胞,通过G418筛选,建立了稳定的MC4R激动剂筛选细胞株.利用MC4R内源激动剂α-MSH探索和优化了每孔接种细胞数目、激动剂孵育时间、溶剂DMSO终浓度、荧光素酶底物浓度等筛选条件,建立了可靠的筛选方法.实验表明：当细胞数目为4×10⁴个/孔,激动剂孵育时间为8 h,每孔DMSO终浓度小于1％和α-MSH终浓度为1 μmol/L时,系统Z'-因子接近0.7,能够用于MC4R激动剂的高通量筛选.     

C57BL/6小鼠听皮层脑片的长时程增强特性

采用脑片细胞外记录群体细胞兴奋性突触后电位方法,在成年C57BL／6小鼠听皮层上,研究长时程增强(10ng-termpotentiation,LTP)特征。用100Hz高频电脉冲刺激听皮层白质,可在听皮层灰质Ⅱ/Ⅲ层记录到明显的LTP。根据条件刺激后LTP的变化特征,将其分为缓慢上升(A类)和短暂快速上升(B类)两种类型。使用模拟的θ节律刺激参数,可更有效地诱导听皮层LTP,其群体细胞兴奋性突触后电位斜率增加更为明显(P＜0．01),诱导成功率也更高。     

播栽期对水稻产量和产量构成因素及生育期的影响

研究了不同播栽期对水稻产量和产量构成因素及生育期的影响。结果表明 ,随播栽期推迟 ,水稻产量有所降低。每穗成粒数减少是推迟播栽期引起水稻减产的主要原因 ,其次是千粒重的下降和成穗数的降低。针对目前的生产实际 ,提出了一些应对播栽期推迟的技术措施。     

A selective resonance scattering assay for immunoglobulin G using Cu(II)-ascorbic acid-immunonanogold reaction

In sodium acetate–acetic acid buffer solution, Au, Ag, Pt, Pd, Fe₃O₄, and Cu₂O nanoparticles have catalytic enhancement effect on the reduction of Cu²⁺ by ascorbic acid to form large copper particles that exhibit a strong resonance scattering peak at 610 nm. Those nanocatalytic reactions were studied by the resonance scattering spectral technique, and smaller nanogold exhibited stronger catalytic enhancement effect in pH 4.2 sodium acetate–acetic acid buffer solution. The resonance scattering intensity at 610 nm increased linearly with the concentrations of 0.02 to 1.60, 0.040 to 1.20, and 0.12 to 4.70 nM nanogold in sizes of 5, 10, and 15 nm with detection limits of 0.010, 0.030, and 0.10 nM, respectively. An immunonanogold-catalytic resonance scattering bioassay was established, combining the immunonanogold-catalytic effect on CuSO₄–ascorbic acid reaction with the resonance scattering detection technique. As a model, 0.03 to 7.5 ng ml⁻¹ immunoglobulin G can be assayed by this immunonanogold-catalytic resonance scattering bioassay with a detection limit of 0.015 ng ml⁻¹.     

High IFN－α expression is associated with the induction of experimental autoimmune uveitis （EAU） in Fischer 344 rat

INTRODUCTIONThe CD4 T cells can be subdivided intoTh1 and Th2 subsets based on their secreted cy-tokine profile. Th1 cells characteristical1y secreteInterferonry (IFN--ry), whereas Th2 cells maiuly pro--duce IL--4[1]. IL-12 po1arizes the differentiation ofnaitre, CD4 T cells towards Th1 pathWay, in con-trast IL--4 directs T cell differentiation towards Th2pathWay The broken balance between Th1 and Th2immune responses and predominant Th1 responseare crucial factors in initiation…     

海南岛霸王岭热带山地雨林群落结构及树种多样性特征的研究

 以海南岛霸王岭自然保护区1 hm2老龄原始林样地的调查材料为基础,分析了热带山地雨林群落的组成、高度结构、径级结构及有关的树种多样性特征。结果表明：霸王岭热带山地雨林树种较丰富,物种多样性指数较高。树种数和树木的密度都随高度级、径级的增加而呈负指数或负幂函数递减;热带山地雨林不同高度级、不同径级和不同小样方斑块内的树种数都与树木密度呈显著的正相关关系。热带山地雨林经过自然的演替达到老龄顶极群落后,最后进入主林层的只是少部分树种的少数个体。     

中国南部高等真菌的热带亲缘

中国南部,特别是北回归线以南的地区,高等真菌区系与世界热带真菌区系有着广泛的联系。该地区高等真菌具有明显的热带性质,主要有泛热带成分、热带亚洲—热带非洲和热带亚洲的成分,尽管在该区高等真菌区系中也有相当比重的温带成分。该区系中某些属种可能有古南大陆起源的背景,另一些区系成分与热带亚洲的有密切的亲缘关系。     

西藏拉萨地区三叠系诺利阶牙形石分带及其国际对比

麦龙岗剖面位于西藏拉萨地区达孜县麦龙岗村 (N2 9°5 6′0 6″ ,E91°2 7′5 5″)。麦龙岗组中共发现牙形石 7属 :Epigondolella ,Hindeodella ,Priniodella ,Xaniognathodus,Lonchodina ,Metalonchodina和Enantiognathus属。其中Epigondolella属有 9种 ,包括 1新种和 1未定种 ,它们是E .primitia ,E .spiculata ,E .cf.spiculata ,E .tozeri,E .violinformissp .nov .,E .cf.triangularisuniformis,E .postera ,E .bidentata ,E .sp .。基于本次发现的化石材料 ,对西藏林周地区麦龙岗组进行新的牙形石带划分。自下而上划分为 :?Epigondolellaprimitia ,E .spiculata ,E .tozer i,E .postera和E .bidentata等带。与毛力等 (1987)、Orchard(1983,1991,1994 )和Krystyn(1977)的划分方案进行对比和讨论