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A A Sergeev Iu G Chernukha K L Shakhanina Z A Gol'denshte?n E Ia Potashnikova 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1989,(7):66-71
The possibility of using the enzyme-linked immunosorbent assay (ELISA) for the diagnosis of leptospirosis has been shown. This method has proved to be more simple and sensitive than the leptospiral microagglutination and lysis test. The data on obtaining genus-specific leptospiral antigens are presented. As revealed in this study, the antigens obtained by the complex treatment of microbial cells with ultrasound and detergents show the maximum activity in ELISA. The optimum parameters of the ELISA system for the diagnosis of leptospirosis have been established. 相似文献
104.
M V Kolmakova Z R Kuskova G M Ratner L M Laptakova 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1989,(5):47-50
Horse serum albumin has been shown to meet the requirements to protein preparations for microanalysis and thus to be suitable for use in kits of reagents for the radioimmunological determination of insulin and myoglobin, for the determination of tick-borne encephalitis virus antigen by the method of the enzyme immunoassay and for the stabilization of proteins in the hemagglutination test and the hemagglutination inhibition test. 相似文献
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Background
Meiotic prophase is a critical stage in sexual reproduction. Aberrant chromosome recombination during this stage is a leading cause of human miscarriages and birth defects. However, due to the experimental intractability of mammalian gonads, only a very limited number of meiotic genes have been characterized. Here we aim to identify novel meiotic genes important in human reproduction through computational mining of cross-species and cross-sex time-series expression data from budding yeast, mouse postnatal testis, mouse embryonic ovary, and human fetal ovary. 相似文献109.
R. A. Tobey H. A. Crissman M. E. Wilder F. Traganos Z. Darzynkiewicz 《Cell proliferation》1987,20(3):363-366
Abstract. A population of line CHO Chinese hamster cells was synchronized by mitotic selection and allowed to enter early G1 , after which the largest and smallest cells in the population were sorted, irradiated, and their viability determined. Despite sizeable differences in volume, metabolic capability and cell cycle progression rates, an equivalent level of survival was obtained for the two populations, indicating that the factors responsible for the volume, metabolic and progression heterogeneity do not contribute greatly to radiation sensitivity. 相似文献
110.