全文获取类型
收费全文 | 101735篇 |
免费 | 7571篇 |
国内免费 | 212篇 |
专业分类
109518篇 |
出版年
2021年 | 1141篇 |
2020年 | 1025篇 |
2019年 | 1006篇 |
2018年 | 2415篇 |
2017年 | 2259篇 |
2016年 | 2945篇 |
2015年 | 3754篇 |
2014年 | 3893篇 |
2013年 | 5097篇 |
2012年 | 6057篇 |
2011年 | 5566篇 |
2010年 | 3542篇 |
2009年 | 2888篇 |
2008年 | 4515篇 |
2007年 | 4313篇 |
2006年 | 4124篇 |
2005年 | 3519篇 |
2004年 | 3408篇 |
2003年 | 3087篇 |
2002年 | 2905篇 |
2001年 | 4113篇 |
2000年 | 3798篇 |
1999年 | 2812篇 |
1998年 | 1032篇 |
1997年 | 834篇 |
1996年 | 787篇 |
1995年 | 698篇 |
1994年 | 621篇 |
1993年 | 559篇 |
1992年 | 1779篇 |
1991年 | 1589篇 |
1990年 | 1474篇 |
1989年 | 1420篇 |
1988年 | 1304篇 |
1987年 | 1258篇 |
1986年 | 1121篇 |
1985年 | 1114篇 |
1984年 | 891篇 |
1983年 | 732篇 |
1982年 | 563篇 |
1979年 | 824篇 |
1978年 | 636篇 |
1975年 | 674篇 |
1974年 | 771篇 |
1973年 | 750篇 |
1972年 | 735篇 |
1971年 | 677篇 |
1970年 | 668篇 |
1969年 | 679篇 |
1968年 | 570篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
Fátima H. Vaz Patrícia M. Machado Rita D. Brand?o Cátia T. Laranjeira Joana S. Eugénio Aires H. Fernandes Saudade P. André 《The journal of histochemistry and cytochemistry》2007,55(11):1105-1113
Only 20-25% of families screened for BRCA1/2 mutations are found positive. Because only a positive result is informative, we studied the role of BRCA1/2 immunohistochemistry as an additional method for patient selection. From 53 high-risk-affected probands, 18 (34%) had available paraffin blocks of their tumors and were selected for this study. Mutation screening was done by conformation-sensitive gel electrophoresis and multiplex ligation-dependent probe amplification. For immunohistochemistry, 21 neoplastic specimens (15 breast carcinomas, 5 ovary neoplasms, and 1 rectal adenocarcinoma) were analyzed with BRCA1 (monoclonal antibody, Ab-1, oncogene) and BRCA2 (polyclonal antibody, Ab-2, oncogene) antibodies. Absence of the BRCA1 protein was confirmed in negative tumors by Western blotting. Seven patients were positive for BRCA1/2 mutations: 5 for BRCA1 and 2 for BRCA2. Four out of five positive patients had tumors negative for BRCA1 immunostaining, and the remaining 13 BRCA1-negative patients had positive BRCA1 immunostaining in all tumor samples. Sensitivity to predict for BRCA1 mutation carriers was 80%, and specificity was 100%, with a positive predictive value of 100% and a negative predictive value of 93%. This correlation was statistically significant (p=0.001). No correlation was observed for BRCA2. If larger studies confirm these results, high-risk patients with BRCA1-negative tumors should be screened first for this gene. 相似文献
102.
Z Jesenská I Sajbidorová 《Journal of hygiene, epidemiology, microbiology, and immunology》1991,35(1):41-49
The biodegradation of T-2 toxin was studied by strains of micromycetes which were isolated from the environment. The 26 tested strains were divided into three groups. Group contains strains which degraded T-2 toxin very fast. This toxin could not be chromatographically determined in the medium even after 48 hours of incubation and the antifungal activity of residua against Kluyveromyces fragilis CCY-51-1-2 was low or zero. There were strains of Alternaria sp., Ulocladium sp., Aspergillus candidus, Cladosporium cladosporioides, Rhodotorula sp., Aspergillus flavus and Cladosporium macrocarpum. Group II contains with a low activity and in group III the results were variable and non stable. 相似文献
103.
104.
Abstract cDNA-RNA liquid hybridization analysis was used to compare the RNA sequence homology between two members of the Nudaurelia β virus family, Trichoplusia ni virus ( T.ni V) and Dasychira pudibunda virus ( D.p V). Heterologous hybridization experiments demonstrated that these viruses shared little sequence homology. Using oligo(dT) chromatography and oligo(dT)12–18 as a primer for cDNA synthesis it was shown that neither T.ni V nor D.p V RNA genomes possess a poly(A) tract at the 3' end. 相似文献
105.
In these studies we describe the production of three mAb raised to an idiotype on an IgG anticasein antibody isolated from the serum of one IgA-deficient blood donor. These are IgM kappa and block the binding of casein Ag to anticasein antibody. Sera of unrelated IgA-deficient donors were tested for the presence of the idiotype; 15 of 56 IgA-deficient sera (25%) contain the anticasein idiotype, whereas 1 of 45 normal sera was positive. Anticasein antibodies as a whole were predominantly of the IgG1 and IgG3 subclass; idiotype-positive anticaseins are predominantly of the IgG1 subclass. For IgA-deficient donors, the relative amount of idiotype-positive anticasein antibody was correlated with the level of anticasein present in the serum. Studies were done to investigate the potential inheritance of the idiotype in families; in three of four families the idiotype was inherited in an apparent autosomal dominant pattern. Our data show that a common cross-reactive idiotype can be detected in the sera of IgA-deficient individuals and their family members. This suggests that V region markers may be conserved in this humoral immunodeficiency disease. 相似文献
106.
107.
108.
I Martínez de Mara?ón N Chaudanson N Joly P Gervais 《Biotechnology and bioengineering》1999,65(2):176-181
Thermal resistance of Saccharomyces cerevisiae was found to be drastically dependent on the kinetics of heat perturbation. Yeasts were found to be more resistant to a plateau of 1 h at 50 degrees C after a slope of temperature increase (slow and linear temperature increments) than after a shock (sudden temperature change). Thermotolerance was mainly acquired between 40-50 degrees C during a heat slope, i.e., above the maximal temperature of growth. The death of the yeasts subjected to a heat shock might be related to the loss of membrane integrity: intracellular contents extrusion, i.e., membrane permeabilization, was found to precede cell death. However, the permeabilization did not precede cell death during a heat slope and, therefore, membrane permeabilization was a consequence rather than a cause of cell death. During a slow temperature increase, yeasts which remain viable may have time to adapt their plasma membrane and thus maintain membrane integrity. 相似文献
109.
110.
Abstract Azide, an inhibitor of ATPase, and a specific inhibitor of protein export was used in order to select for protein secretion mutants in Acinetobacter calcoaceticus A2. Two such mutants were isolated that were azide-resistant and defective in the general protein transport system. The mutation also conferred additional phenotypic changes, including an inability to grow on minimal media or at 40°C. The existence of protein secretion mutants with a selectable phenotype may be useful for the genetic study of protein export. 相似文献