全文获取类型
收费全文 | 2730篇 |
免费 | 244篇 |
国内免费 | 1篇 |
专业分类
2975篇 |
出版年
2023年 | 7篇 |
2022年 | 23篇 |
2021年 | 52篇 |
2020年 | 24篇 |
2019年 | 26篇 |
2018年 | 44篇 |
2017年 | 33篇 |
2016年 | 78篇 |
2015年 | 145篇 |
2014年 | 143篇 |
2013年 | 185篇 |
2012年 | 236篇 |
2011年 | 229篇 |
2010年 | 143篇 |
2009年 | 130篇 |
2008年 | 169篇 |
2007年 | 174篇 |
2006年 | 136篇 |
2005年 | 139篇 |
2004年 | 133篇 |
2003年 | 114篇 |
2002年 | 107篇 |
2001年 | 34篇 |
2000年 | 22篇 |
1999年 | 20篇 |
1998年 | 36篇 |
1997年 | 27篇 |
1996年 | 25篇 |
1995年 | 34篇 |
1994年 | 23篇 |
1993年 | 17篇 |
1992年 | 22篇 |
1991年 | 19篇 |
1990年 | 12篇 |
1989年 | 13篇 |
1988年 | 10篇 |
1987年 | 10篇 |
1986年 | 9篇 |
1985年 | 17篇 |
1984年 | 10篇 |
1983年 | 12篇 |
1982年 | 13篇 |
1981年 | 7篇 |
1980年 | 9篇 |
1979年 | 10篇 |
1978年 | 9篇 |
1977年 | 8篇 |
1976年 | 17篇 |
1975年 | 13篇 |
1969年 | 7篇 |
排序方式: 共有2975条查询结果,搜索用时 46 毫秒
31.
32.
33.
34.
Extreme temperatures,foundation species,and abrupt ecosystem change: an example from an iconic seagrass ecosystem 下载免费PDF全文
Jordan A. Thomson Derek A. Burkholder Michael R. Heithaus James W. Fourqurean Matthew W. Fraser John Statton Gary A. Kendrick 《Global Change Biology》2015,21(4):1463-1474
Extreme climatic events can trigger abrupt and often lasting change in ecosystems via the reduction or elimination of foundation (i.e., habitat‐forming) species. However, while the frequency/intensity of extreme events is predicted to increase under climate change, the impact of these events on many foundation species and the ecosystems they support remains poorly understood. Here, we use the iconic seagrass meadows of Shark Bay, Western Australia – a relatively pristine subtropical embayment whose dominant, canopy‐forming seagrass, Amphibolis antarctica, is a temperate species growing near its low‐latitude range limit – as a model system to investigate the impacts of extreme temperatures on ecosystems supported by thermally sensitive foundation species in a changing climate. Following an unprecedented marine heat wave in late summer 2010/11, A. antarctica experienced catastrophic (>90%) dieback in several regions of Shark Bay. Animal‐borne video footage taken from the perspective of resident, seagrass‐associated megafauna (sea turtles) revealed severe habitat degradation after the event compared with a decade earlier. This reduction in habitat quality corresponded with a decline in the health status of largely herbivorous green turtles (Chelonia mydas) in the 2 years following the heat wave, providing evidence of long‐term, community‐level impacts of the event. Based on these findings, and similar examples from diverse ecosystems, we argue that a generalized framework for assessing the vulnerability of ecosystems to abrupt change associated with the loss of foundation species is needed to accurately predict ecosystem trajectories in a changing climate. This includes seagrass meadows, which have received relatively little attention in this context. Novel research and monitoring methods, such as the analysis of habitat and environmental data from animal‐borne video and data‐logging systems, can make an important contribution to this framework. 相似文献
35.
Golden bananas in the field: elevated fruit pro‐vitamin A from the expression of a single banana transgene 下载免费PDF全文
Jean‐Yves Paul Harjeet Khanna Jennifer Kleidon Phuong Hoang Jason Geijskes Jeff Daniells Ella Zaplin Yvonne Rosenberg Anthony James Bulukani Mlalazi Pradeep Deo Geofrey Arinaitwe Priver Namanya Douglas Becker James Tindamanyire Wilberforce Tushemereirwe Robert Harding James Dale 《Plant biotechnology journal》2017,15(4):520-532
Vitamin A deficiency remains one of the world's major public health problems despite food fortification and supplements strategies. Biofortification of staple crops with enhanced levels of pro‐vitamin A (PVA) offers a sustainable alternative strategy to both food fortification and supplementation. As a proof of concept, PVA‐biofortified transgenic Cavendish bananas were generated and field trialed in Australia with the aim of achieving a target level of 20 μg/g of dry weight (dw) β‐carotene equivalent (β‐CE) in the fruit. Expression of a Fe'i banana‐derived phytoene synthase 2a (MtPsy2a) gene resulted in the generation of lines with PVA levels exceeding the target level with one line reaching 55 μg/g dw β‐CE . Expression of the maize phytoene synthase 1 (ZmPsy1) gene, used to develop ‘Golden Rice 2’, also resulted in increased fruit PVA levels although many lines displayed undesirable phenotypes. Constitutive expression of either transgene with the maize polyubiquitin promoter increased PVA accumulation from the earliest stage of fruit development. In contrast, PVA accumulation was restricted to the late stages of fruit development when either the banana 1‐aminocyclopropane‐1‐carboxylate oxidase or the expansin 1 promoters were used to drive the same transgenes. Wild‐type plants with the longest fruit development time had also the highest fruit PVA concentrations. The results from this study suggest that early activation of the rate‐limiting enzyme in the carotenoid biosynthetic pathway and extended fruit maturation time are essential factors to achieve optimal PVA concentrations in banana fruit. 相似文献
36.
Quantitative comparison of Fusarium oxysporum competitiveness in relation to carbon utilization 总被引:3,自引:0,他引:3
Abstract The competitive saprophytic interaction between pathogenic and non-pathogenic strains of Fusarium oxysporum was investigated by studying their ability to competitively colonize sterilized soil. It was demonstrated that carbon was the first limiting substrate of Fusarium oxysporum growth in sterilized soil. Moreover, the efficiency with which glucose was utilized in the formation of propagules varied from one strain to another. Results of competitiion experiments showed that a linear relationship existed between the ratio of inoculum densities at the plateau (carrying capacity) and the ratio of inoculum densities incorporated into non-amended sterilized soil. The slope of the regression line indicated the competitiveness index, i.e. the competitiveness of a non-pathogenic strain in relation to that of a pathogenic strain. This parameter could be related to the yield coefficient of the strains in glucose-amended soil. 相似文献
37.
38.
39.
Advances in synthetic biology are contributing to diverse research areas, from basic biology to biomanufacturing and disease
therapy. We discuss the theoretical foundation, applications, and potential of this emerging field. 相似文献
40.
Proteome analysis reveals novel proteins associated with proliferation and differentiation of the colorectal cancer cell line Caco-2 总被引:26,自引:0,他引:26
Stierum R Gaspari M Dommels Y Ouatas T Pluk H Jespersen S Vogels J Verhoeckx K Groten J van Ommen B 《Biochimica et biophysica acta》2003,1650(1-2):73-91
Here, we describe a proteomics approach to study protein expression changes in differentiating Caco-2 cells. Caco-2 is a colorectal carcinoma cell line, which upon differentiation loses its tumorigenic phenotype and displays characteristics of mature enterocytes, including brush borders with microvilli. Cells were grown in culture flasks and harvested at different stages of differentiation (days post-confluence: -3, 0, 3, 7, 10, 14, and 18). Two-dimensional gel electrophoresis was used to analyse proteome changes. Approximately 1400 protein spots were detected within the Caco-2 proteome, within the pH 4-7 range. Two-dimensional gel electrophoresis allowed for the detection of 18 proteins from which the levels of expression were found to be associated with differentiation. Of these proteins, 11 were identified by means of MALDI-TOF or NANO-ESI-MS/MS mass spectrometry and include liver fatty acid binding protein (FABL), three forms of alpha-enolase (ENOA), nucleoside diphosphate kinase A (NDKA), cofilin-1 (COF1), translationally controlled tumour protein (TCTP), mitochondrial 60-kDa heat shock protein (CH60), probable protein disulfide isomerase (ER60), creatine kinase B (KCRB), and glutathione S-transferase alpha (GTA1). Thus, proteomics revealed that the differentiation-related change in phenotype of Caco-2 involves changes in a variety of distinct biochemical pathways. Some of these proteins have not been shown before to be associated with Caco-2 differentiation (ER60; COF1; CH60; NDKA; TCTP and ENOA). Therefore, processes related to protein folding and disulfide bridge formation, cytoskeleton formation and maintenance, nucleotide metabolism, glycolysis as well as tumorigenesis-associated proteins may be involved in Caco-2 differentiation. Changes in the expression of CH60, TCTP, GTA1, NDKA, and FABL have also been reported to be associated with in vivo colon carcinogenesis. These findings illustrate that a combination of proteomics and cell culture is a useful approach to find markers for Caco-2 differentiation, which could contribute to the comprehension of the process of colon carcinogenesis. 相似文献