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排序方式: 共有453条查询结果,搜索用时 625 毫秒
101.
Yanjie Chen Xinzhao Zuo Qinglv Wei Jie Xu Xiaoyi Liu Shiling Liu Haocheng Wang Qingya Luo Yuya Wang Yu Yang Hongyan Zhao Jing Xu Tao Liu Ping Yi 《International journal of biological sciences》2023,19(2):691
Cervical cancer (CC) is one of the most common gynecological malignancies with poor prognosis for advanced CC patients. LRRC8A is a volume-regulated anion channel protein involved in cellular homeostasis, but its role in CC remains largely unknown. In this study, we found that LRRC8A is elevated in CC and associated with poor prognosis. LRRC8A maintains cell survivals under the hypotonic condition, and promotes tumorigenesis through apoptosis suppression in vitro and in vivo. Notably, LRRC8A is upregulated by NSUN2-mediated m5C modification. m5C modified-LRRC8A mRNA is bound by the RNA binding protein YBX1 followed by the increased RNA stability. Moreover, loss of NSUN2 suppresses the proliferation and metastasis of CC cells, and NSUN2 expression is positively correlated with LRRC8A expression in CC. Altogether, our study demonstrates that the NSUN2-m5C-LRRC8A axis is crucial and would be a potential therapeutic target for CC. 相似文献
102.
Perdeuteration in neutron crystallography is an effective method for determining the positions of hydrogen atoms in proteins. However, there is shortage of evidence that the high-resolution details of perdeuterated proteins are consistent with those of the nondeuterated proteins. In this study, we determined the X-ray structure of perdeuterated high-potential iron-sulfur protein (HiPIP) at a high resolution of 0.85 å resolution. The comparison of the nondeuterated and perdeuterated structures of HiPIP revealed slight differences between the two structures. The spectroscopic and spectroelectrochemical studies also showed that perdeuterated HiPIP has approximately the same characteristics as nondeuterated HiPIP. These results further emphasize the suitability of using perdeuterated proteins in the high-resolution neutron crystallography. 相似文献
103.
Yuya Takahashi Xianghua Li Chigen Tsukamoto Kejing Wang 《Molecular breeding : new strategies in plant improvement》2018,38(7):93
Group A saponins are thought to be the cause of bitter and astringent tastes in processed foods of soybean (Glycine max), and the elimination of group A saponins is an important breeding objective. The group A saponins include two main Aa and Ab types, controlled by codominant alleles at the Sg-1 locus that is one of several key loci responsible for saponin biosynthesis in the subgenus Glycine soja. However, A0 mutant lacking group A saponin is a useful gene resource for soybean quality breeding. Here, eight Chinese wild soybean A0 accessions were sequenced to reveal the mutational mechanisms, and the results showed that these mutants were caused by at least three kinds of mechanisms involving four allelic variants (sg-10-b2, sg-10-b3, Sg-1b-0, and Sg-1b-01). The sg-10-b2 had two nucleotide deletions at positions +?72 and +?73 involving in the 24th and 25th amino acids. The sg-10-b3 contained a stop codon (TGA) at the 254th residue. The Sg-1b-0 and Sg-1b-01 were two novel A0-type mutants, which likely carried normal structural alleles, and nevertheless did not encode group A saponin due to unknown mutations beyond the normal coding regions. In addition, to reveal the structural features, allelic polymorphism, and mechanisms of the abiogenetic absence of group A (i.e., A0 phenotype), nucleotide sequence analysis was performed for the Sg-1 locus in wild soybean (Glycine soja). The results showed that Sg-1 alleles had a lower conservatism in the coding region; as high as 18 sequences were found in Chinese wild soybeans in addition to the Sg-1a (Aa) and Sg-1b (Ab) alleles. Sg-1a and Sg-1b alleles were characterized by eight synonymous codons and nine amino acid substitutions. Two evolutionarily transitional allelic sequences (Sg-1a7 and Sg-1b2) from Sg-1a toward Sg-1b were detected. 相似文献
104.
Sosuke Yoshinaga Norihito Ishida Tatsuichiro Tsuji Akihiro Sonoda Kaori Yunoki Mitsuhiro Takeda Etsuko Toda Yuya Terashima Kouji Matsushima Hiroaki Terasawa 《Biomolecular NMR assignments》2018,12(2):259-262
FROUNT is a cytoplasmic protein that interacts with the membrane-proximal C-terminal regions (Pro-Cs) of the CCR2 and CCR5 chemokine receptors. The interactions between FROUNT and the chemokine receptors play an important role in the migration of inflammatory immune cells. Therefore, FROUNT is a potential drug target for inflammatory diseases. However, the structural basis of the interactions between FROUNT and the chemokine receptors remains to be elucidated. We previously identified the C-terminal region (residues 532–656) of FROUNT as the structural domain responsible for the Pro-C binding, referred to as the chemokine receptor-binding domain (CRBD), and then constructed its mutant, bearing L538E/P612S mutations, with improved NMR spectral quality, referred to as CRBD_LEPS. We now report the main-chain and side-chain 1H, 13C, and 15N resonance assignments of CRBD_LEPS. The NMR signals of CRBD_LEPS were well dispersed and their intensities were uniform on the 1H–15N HSQC spectrum, and thus almost all of the main-chain and side-chain resonances were assigned. This assignment information provides the foundation for NMR studies of the three-dimensional structure of CRBD_LEPS in solution and its interactions with chemokine receptors. 相似文献
105.
Kae?Wakabayashi Michio?Oguro Tomoyuki?Itagaki Satoki?SakaiEmail authorView authors OrcID profile 《Plant Ecology》2018,219(8):985-997
Little is known about how plants protect flowers—their reproductive organs—against florivory. Additionally, the induced floral defense system has been examined in only a few species. We tested the inducibility of putative floral defenses and investigated the relationship between natural florivory and the floral defenses of 12 naturally growing plant species. The relationships between florivory and four chemical traits (nitrogen, phosphorus, total phenolics, and condensed tannins) were investigated in 12 plant species. We also studied whether flowers induce changes in chemical defenses in response to artificial damage in 10 plant species. A higher concentration of floral nitrogen was associated with a decreasing frequency of florivore attacks. Among the four traits of the 10 plant species studied, no trait changed in response to the artificial damage. We suggest that induced defense systems may not be advantageous for flowers, although it is also possible that these species simply do not use induced defense in any of their plant parts. 相似文献
106.
Takeshi Minami Yuko Okazaki Hisanao Komiya Yoshitaka Horiuchi Toru Inoue Yuya Yamada Hisako Fushimi 《Biological trace element research》1995,49(1):67-73
Calcification occurred in the kidney of streptozotocin (STZ)-induced diabetic rats fed a low-zinc diet. The deposits were
stained by the von Kossa method and were detected intracellularly in the tubular cells, mainly in the cortico-medullar region.
The deposits were found to be a heterogenous substance on electron microscopy. There were various sizes of deposits, and the
surfactant was very much distorted. Many deposits grew up to bind small particles, and the core-like substance was observed
in the center of the deposit. The component of the deposit was analyzed by an X-ray microanalyzer, and was found to be calcium
and phosphorus. The ratio of calcium to phosphorus was 2.159, which coincided with the ratio of standard hydroxyapatite. From
these observations, the deposit is believed to be hydroxyapatite. It is thought that the core is formed at first, many particles
are then bound to the core, and deposits grow up. 相似文献
107.
108.
Convective and radiative heat transfer coefficients for individual human body segments 总被引:6,自引:0,他引:6
R. J. de Dear Edward Arens Zhang Hui Masayuki Oguro 《International journal of biometeorology》1997,40(3):141-156
Human thermal physiological and comfort models will soon be able to simulate both transient and spatial inhomogeneities in
the thermal environment. With this increasing detail comes the need for anatomically specific convective and radiative heat
transfer coefficients for the human body. The present study used an articulated thermal manikin with 16 body segments (head,
chest, back, upper arms, forearms, hands, pelvis, upper legs, lower legs, feet) to generate radiative heat transfer coefficients
as well as natural- and forced-mode convective coefficients. The tests were conducted across a range of wind speeds from still
air to 5.0 m/s, representing atmospheric conditions typical of both indoors and outdoors. Both standing and seated postures
were investigated, as were eight different wind azimuth angles. The radiative heat transfer coefficient measured for the whole-body
was 4.5 W/m2 per K for both the seated and standing cases, closely matching the generally accepted whole-body value of 4.7 W/m2 per K. Similarly, the whole-body natural convection coefficient for the manikin fell within the mid-range of previously published
values at 3.4 and 3.3 W/m2 per K when standing and seated respectively. In the forced convective regime, heat transfer coefficients were higher for hands,
feet and peripheral limbs compared to the central torso region. Wind direction had little effect on convective heat transfers
from individual body segments. A general-purpose forced convection equation suitable for application to both seated and standing
postures indoors was h
c=10.3v
0.6 for the whole-body. Similar equations were generated for individual body segments in both seated and standing postures.
Received: 21 May 1996/Accepted: 27 November 1996 相似文献
109.
110.
Dr. Masatoshi Ito Hideo Tsuda Katsuhiko Oguro Kozo Mutoh Hideyuki Shiraishi Yukiyoshi Shirasaka Haruki Mikawa 《Neurochemical research》1990,15(9):933-936
GABA-gated chloride ion influx was measured in brain microsac preparations of epileptic El mice. There was significantly greater sensitivity to GABA in stimulated El mice (which had 14–18 convulsions induced at weekly intervals) than in unstimulated El mice (which had not experienced convulsions) or ddY mice. GABA-gated chloride ion influx was significantly decreased 20 min after a single convulsion, and returned to the preconvulsion level 60 min after a convulsion. These findings suggest that the functional state of GABA-gated chloride channel in El mice is changed secondarily by single or repeated convulsions. 相似文献