Cost of defensive spraying by larval Osmylus hyalinatus (Neuroptera: Osmylidae) for post-larval development

Journal of Ethology - The chemical defense of insects is effective for avoiding predation, but may carry a cost in terms of life history traits. If chemical defenses require the resources and/or...     

Local factors affecting winter habitat use of non-native rainbow trout in a boreal stream in northern Japan

Ichthyological Research - Despite its potential use for population control, the winter ecology of nonnative fishes is still poorly understood due to the difficulty of conducting field surveys. In...     

Description of Optioservus gapyeongensis new species and Heterlimnius hasegawai (Nomura) (Coleoptera: Elmidae) new to Korea

A new riffle beetle species Optioservus gapyeongensis n. sp. is described from the adult and larval stages. Heterlimnius hasegawai (Nomura), previously misidentified in Korea as Optioservus (Cyclolimnius) kubotai Nomura or Optioservus variabilis Nomura, is redescribed. The adults and larvae of both species inhabit attached mosses on substrates of clean mountain streams, headwaters or springs. Photographs of dorsal adults and habitats and line drawings of diagnostic characters are provided.     

Metabolic engineering to improve 1,5-diaminopentane production from cellobiose using β-glucosidase-secreting Corynebacterium glutamicum

Microbial production of 1,5-diaminopentane (DAP) from renewable feedstock is a promising and sustainable approach for the production of polyamides. In this study, we constructed a β-glucosidase (BGL)-secreting Corynebacterium glutamicum and successfully used this strain to produce DAP from cellobiose and glucose. First, C. glutamicum was metabolically engineered to produce l -lysine (a direct precursor of DAP), followed by the coexpression of l -lysine decarboxylase and BGL derived from Escherichia coli and Thermobifida fusca YX (Tfu0937), respectively. This new engineered C. glutamicum strain produced 27 g/L of DAP from cellobiose in CGXII minimal medium using fed-batch cultivation. The yield of DAP was 0.43 g/g glucose (1 g of cellobiose corresponds to 1.1 g of glucose), which is the highest yield reported to date. These results demonstrate the feasibility of DAP production from cellobiose or cellooligosaccharides using an engineered C. glutamicum strain.