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91.
Kawarazaki H Ando K Fujita M Matsui H Nagae A Muraoka K Kawarasaki C Fujita T 《American journal of physiology. Renal physiology》2011,300(6):F1402-F1409
Excessive salt intake is known to preferentially increase blood pressure (BP) and promote kidney damage in young, salt-sensitive hypertensive human and animal models. We have suggested that mineralocorticoid receptor (MR) activation plays a major role in kidney injury in young rats. BP and urinary protein were compared in young (3-wk-old) and adult (10-wk-old) uninephrectomized (UNx) Sprague-Dawley rats fed a high (8.0%)-salt diet for 4 wk. The effects of the MR blocker eplerenone on BP and renal injury were examined in the high-salt diet-fed young UNx rats. Renal expression of renin-angiotensin-aldosterone (RAA) system components and of inflammatory and oxidative stress markers was also measured. The effects of the angiotensin receptor blocker olmesartan with or without low-dose aldosterone infusion, the aldosterone synthase inhibitor FAD286, and the antioxidant tempol were also studied. Excessive salt intake induced greater hypertension and proteinuria in young rats than in adult rats. The kidneys of young salt-loaded rats showed marked histological injury, overexpression of RAA system components, and an increase in inflammatory and oxidative stress markers. These changes were markedly ameliorated by eplerenone treatment. Olmesartan also ameliorated salt-induced renal injury but failed to do so when combined with low-dose aldosterone infusion. FAD286 and tempol also markedly reduced urinary protein. UNx rats exposed to excessive salt at a young age showed severe hypertension and renal injury, likely primarily due to MR activation and secondarily due to angiotensin receptor activation, which may be mediated by inflammation and oxidative stress. 相似文献
92.
Sato K Horiuchi Y Jin Y Malchinkhuu E Komachi M Kondo T Okajima F 《Journal of neurochemistry》2011,117(1):164-174
Action mechanism of lipopolysaccharide (LPS), interleukin-1β (IL-1β), and lysophosphatidic acid (LPA) to regulate motility, an important process of astrogliosis, was investigated in rat astrocytes. While LPA exerted no significant effect on the cell migration, the prior treatment of the cells with LPS or IL-1β resulted in the appearance of migration activity in response to LPA. The LPS induction of the migration response to LPA was associated with the production of IL-1β precursor protein and inhibited by the IL-1 receptor antagonist. The IL-1β treatment also allowed LPA to activate Rac1. The LPA-induced Rac1 activation and migration were inhibited by pertussis toxin, a small interfering RNA specific to LPA(1) receptors, and LPA(1) receptor antagonists, including Ki16425. However, the IL-1β treatment had no appreciable effect on LPA(1) receptor mRNA expression and LPA-induced activation of ERK, Akt, and proliferation. The induction of the migration response to LPA by IL-1β was inhibited by a constitutively active RhoA. Moreover, LPA significantly activated RhoA through the LPA(1) receptor in the control cells but not in the IL-1β-treated cells. These results suggest that IL-1β inhibits the LPA(1) receptor-mediated Rho signaling through the IL-1 receptor, thereby disclosing the LPA(1) receptor-mediated G(i) protein/Rac/migration pathway. 相似文献
93.
Hau Duc Tran Izumi Kinoshita Kensaku Azuma Tomoaki Iseki Yuta Yagi Jun-ichi Nunobe Thuy Thi Ta 《Environmental Biology of Fishes》2014,97(12):1387-1396
To examine regional specific diversity in development and growth of Ayu (Plecoglossus altivelis) larvae, we collected and compared collections from the Kalong estuary in Vietnam, and the Shimanto and Muko estuary, and the Niigata coast in Japan. Among the four areas, most of the morphometrics through ontogeny were similar except that the snout tended to be shorter and the anus hardly migrated in Kalong larvae. The snout length increased gradually with growth in the Vietnamese larvae, while this value increased significantly until ca. 10 mm BL, subsequently being constant up to 30 mm body length (BL) in the Japanese larvae. The water temperature when the larvae were collected was higher in the Kalong than in most of the Japan sites. Growth-rates estimated from otolith increments were from highest to lowest, Niigata (mean?=?0.54 mm/day), Kalong (0.47), Shimanto (0.38) and Muko (0.34). The higher growth-rates were obtained not in Niigata of highest latitudinal region, but in Kalong of lowest latitudinal region. This indicates that Ayu could experience their early developmental stages from the cool temperate to tropical regions, implying the potential biodiversity of this fish species in the world. 相似文献
94.
Yoko Matsumura Kazunori Yoshizawa Ryuichiro Machida Yuta Mashimo Romano Dallai Marco Gottardo Thomas Kleinteich Jan Michels Stanislav N. Gorb Rolf G. Beutel 《Biological journal of the Linnean Society. Linnean Society of London》2014,112(1):40-54
Very unusual genitalia of the species Zorotypus caudelli are described. It contains the unique configuration of two different intromittent organs, one of them strongly elongated. Hyper elongated genitalia are known in different groups of insects. Males have to accommodate these unwieldy structures in the limited spaces of the abdomen and manipulate them acutely during copulation. A crucial question is how do species with elongated genitalia cope with these requirements? To investigate this, we studied key features enabling storage, insertion, and withdrawal of the elongated genitalia. The co‐existence of an elongated narrow tube and a bulky spermatophore is a highly unusual and apparently paradoxical condition. However, we demonstrate that the tube is not involved in sperm transmission, whereas the large spermatophore is transferred to females by a membranous fold of the genitalia. The movement of the spermatophore is caused by haemolymph pressure, which likely also promotes the insertion of both intromittent organs. A comparison with the genital anatomy and reproductive mode in related groups suggests that the elongated tube and its accommodating pouch is a de novo structure, and that the ancestral sperm transport via spermatophore is a preadaptive condition for the acquisition of this unusual structure. © 2014 The Linnean Society of London, Biological Journal of the Linnean Society, 2014, 112 , 40–54. 相似文献
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97.
Solar ultraviolet-B (UVB) radiation has deleterious effects on plant-dwelling mites. We assessed the biological effects of
UVB radiation on the eggs of the twospotted spider mite, Tetranychus urticae Koch, under both near ambient (UV+) and UV-attenuated (UV−) conditions from spring to autumn and compared them to the effects
of temperature and humidity. The ambient daily UVB irradiance increased from January to August and then decreased rapidly
until December, whereas egg hatchability under UV+ was lowest in April (10.7%) and increased almost linearly until October
(74.9–92.3%). In contrast, hatchability under UV− was consistently high (96.2–99.8%) through all seasons. For UV+, the stepwise
multiple linear regression analysis supported the negative correlation of hatchability with cumulative UVB irradiance during
egg periods (cumulative dose), but did not support that with the mean daily UVB irradiance (dose rate), suggesting that UVB-induced
mortality in T. urticae eggs is cumulative dose dependent rather than dose rate dependent. The high mortality in April may have reflected the slower
development caused by the relatively lower temperature and higher UVB radiation, increasing the cumulative dose, while the
low mortality in October may have reflected the faster development caused by the relatively higher temperature and lower UVB
radiation, decreasing the cumulative dose. 相似文献
98.
99.
Karyotypes of four Chinese species of field mice of the genus Apodemus were examined, including Apodemus chevrieri(diploid chromosome number,2 n=48, fundamental number of autosomal arms,FNa=56), A. draco(2 n=48, FNa=48), A. ilex(2 n=48, FNa=48), and A. latronum(2 n=48, FNa=48).Karyotypes of A. chevrieri, A. draco, and A. ilex are reported here for the first time, providing useful information for their species taxonomy. Determining the karyotypes of all species of Apodemus in Asia,both in this and previous studies, provides a solid overview of the chromosome evolution and species differentiation of the genus in East Asia. In addition to allopatric speciation, chromosome rearrangements likely played an important role in the formation of the four Apodemus species groups as well as speciation within each group in East Asia. For example, increased centromeric heterochromatin in A. latronum may have contributed to the post-mating reproductive isolation from the A. draco-A. ilex-A. semotus clade. 相似文献
100.
In the present study, formaldehyde dismutase from Methylobacterium sp. FD1 was partially purified and analyzed by nanoLC–MS/MS; it was then cloned from the genomic DNA of FD1 by PCR. The open reading frame of the formaldehyde dismutase gene of FD1 was estimated to be 1203 bp in length. The molecular weight and pI of formaldehyde dismutase (401 aa), as deduced from the FD1 gene, were calculated at 42,877.32 and 6.56, respectively. NAD(H)-binding residues and zinc-binding residues were found in the amino acid sequence of the deduced formaldehyde dismutase of FD1 by BLAST search. The resting Escherichia coli cells that were transformed with the FD1 formaldehyde dismutase gene degraded high concentrations of formaldehyde and produced formic acid and methanol that were molar equivalents of one-half of the degraded formaldehyde. The lyophilized cells of the recombinant E. coli also degraded high concentrations of formaldehyde. 相似文献