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911.
Tsuneo Kagawa Takashi Fukinbaba Yusuke Sumiki 《Bioscience, biotechnology, and biochemistry》2013,77(4):285-291
The production of gibberellin A7 by Gibberella fujikuroi was studied by using newly devised assay method. Gibberellin A7 increased at preferable temperature range between 32°C and 34°C at the controlled pH(6.0~7.5). The improved isolation process by using column chromatography composed of granular charcoal was found to be extremely convenient, because of its quick elution with satisfactory separation from gibberellic acid which is always accompanied by gibberellin A7 in culture medium. 相似文献
912.
Yusuke Hanaki Ryo C. Yanagita Takahiro Sugahara Misako Aida Harukuni Tokuda Nobutaka Suzuki 《Bioscience, biotechnology, and biochemistry》2013,77(6):888-895
Aplog-1 is a simplified analog of the tumor-promoting aplysiatoxin with anti-proliferative and cytotoxic activities against several cancer cell lines. Our recent findings have suggested that protein kinase Cδ (PKCδ) could be one of the target proteins of aplog-1. In this study, we synthesized amide-aplog-1 (3), in which the C-1 ester group was replaced with an amide group, to improve chemical stability in vivo. Unfortunately, 3 exhibited seventy-fold weaker binding affinity to the C1B domain of PKCδ than that of aplog-1, and negligible anti-proliferative and cytotoxic activities even at 10?4 M. A conformational analysis and density functional theory calculations indicated that the stable conformation of 3 differed from that of aplog-1. Since 27-methyl and 27-methoxy derivatives (1, 2) without the ability to bind to PKC isozymes exhibited marked anti-proliferative and cytotoxic activities at 10?4 M, 3 may be an inactive control to identify the target proteins of aplogs. 相似文献
913.
Eriko Yoshizawa Mai Kaizuka Ayumi Yamagami Mieko Higuchi-Takeuchi Minami Matsui Yusuke Kakei 《Bioscience, biotechnology, and biochemistry》2013,77(3):420-429
Brassinosteroids are plant steroid hormones that regulate plant organs and chloroplast development. The detailed molecular mechanism for plant development by BR signaling is yet to be revealed, and many points regarding the relationship between BR signaling and chloroplast development remain unknown. We identify here the dominant mutant Brz-insensitive-pale green3-1D (bpg3-1D) from the Arabidopsis FOX lines that show reduced sensitivity to the chlorophyll accumulation promoted by the BR biosynthesis inhibitor, Brassinazole (Brz), in the light. BPG3 encodes a novel chloroplast protein that is evolutionally conserved in bacteria, algae, and higher plants. The expression of BPG3 was induced by light and Brz. The inhibition of electron transport in photosystem II of the chloroplasts was detected in bpg3-1D. These results suggest that BPG3 played an important role in regulating photosynthesis in the chloroplast under BR signaling. 相似文献
914.
Biochemical Studies on “Bakanae” Fungus. Part 70–71. Synthesis of Substances related to Gibberellins
Kenji Mori Tomoya Ogawa Masanao Matsui Yusuke Sumiki 《Bioscience, biotechnology, and biochemistry》2013,77(4):239-247
Several gibbane-10-carboxylic acids and esters, including methyl (±)-desoxogibberate and methyl (±))-desoxoepigibberate, were synthesized. 相似文献
915.
Kenji Mori Masanao Matsui Yusuke Sumiki 《Bioscience, biotechnology, and biochemistry》2013,77(3):179-183
Degradation of gibberellin C afforded methyl 1,7-dimethyl-2α-hydroxy-8-oxo-7α-gibb-4a(4b)-ene-1,10-dicarboxylate, from which gibberellin C was reconstructed. Reactions of desoxogibberellin C are also described. 相似文献
916.
Hongmin Zhen Koichi Nakamura Yasuyuki Kitaura Yoshihiro Kadota Takuya Ishikawa Yusuke Kondo 《Bioscience, biotechnology, and biochemistry》2013,77(12):2057-2062
Plasma concentrations of amino acids reflect the intracellular amino acid pool in mammals. However, the regulatory mechanism requires clarification. In this study, we examined the effect of leucine administration on plasma amino acid profiles in mice with and without the treatment of 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH) or rapamycin as an inhibitor of system L or mammalian target of rapamycin complex 1, respectively. The elevation of plasma leucine concentration after leucine administration was associated with a significant decrease in the plasma concentrations of isoleucine, valine, methionine, phenylalanine, and tyrosine; BCH treatment almost completely blocked the leucine-induced decrease in plasma amino acid concentrations. Rapamycin treatment had much less effects on the actions of leucine than BCH treatment. These results suggest that leucine regulates the plasma concentrations of branched-chain amino acids, methionine, phenylalanine, and tyrosine, and that system L amino acid transporters are involved in the leucine action. 相似文献
917.
Kenji Mori Makoto Matsui Yusuke Sumiki 《Bioscience, biotechnology, and biochemistry》2013,77(1):22-34
A synthesis of a compound of gibberic acid type is described. 相似文献
918.
Yoko Tanaka Tomoe Nose Yusuke Jikumaru Yuji Kamiya 《The Plant journal : for cell and molecular biology》2013,74(3):448-457
The number and density of stomata are controlled by endogenous and environmental factors. Despite recent advances in our understanding of stomatal development, mechanisms which prevent stomatal‐lineage entry remain unclear. Here, we propose that abscisic acid (ABA), a phytohormone known to induce stomatal closure, limits initiation of stomatal development and induces enlargement of pavement cells in Arabidopsis cotyledons. An ABA‐deficient aba2‐2 mutant had an increased number/proportion of stomata within a smaller cotyledon, as well as reduced expansion of pavement cells. This tendency was reversed after ABA application or in an ABA over‐accumulating cyp707a1cyp707a3 doublemutant. Our time course analysis revealed that aba2‐2 shows prolonged formation of meristemoids and guard mother cells, both precursors of stoma. This finding is in accordance with prolonged gene expression of SPCH and MUTE, master regulators for stomatal formation, indicating that ABA acts upstream of these genes. Only aba2‐2 mute, but not aba2‐2 spch double mutant showed additive phenotypes and displayed inhibition of pavement cell enlargement with increased meristemoid number, indicating that ABA action on pavement cell expansion requires the presence of stomatal‐lineage cells. 相似文献
919.
Hiroyuki Konno Yusuke Otsuki Kenta Matsuzaki Kazuto Nosaka 《Bioorganic & medicinal chemistry letters》2013,23(14):4244-4247
Synthesis and antifungal activity of cyclic octapeptide derivatives of burkholdines are described. To construct cyclic octapeptides, the combination of Fmoc-SPPS and cyclization with DIC/HOBt in the solution phase was employed. Synthesized peptides were evaluated for antifungal activity with MIC values against Saccharomyces cerevisiae, Aspergillus oryzae, and Candida viswanathii. As a result, the lipid side chain and the stereochemistry of each amino acid of Bk-1097 analogues significantly affected antifungal activity. 相似文献
920.