A new species of the genus Malakichthys (Perciformes: Acropomatidae) from Japan

A new acropomatid, Malakichthys barbatus, is described on the basis of 27 specimens (76.3–180.1 mm SL) collected from Suruga Bay to Tosa Bay, along the southern Pacific coast of Japan. The new species is unique in having numerous pairs of spines on the chin (a pair of spines in other congeners). Although the new species resembles M. elegans in having a slender body (the depth less than 35% SL), the former is further distinguishable from the latter in having a slender first proximal radial of the anal fin with no hollow (broad with a cone-shaped hollow in M. elegans), 43–48 lateral line scales (48–51), modally 21 gill rakers on the lower arm (modally 23), and a dark blotch on the opercle (no remarkable blotch). Received: July 2, 2000 / Revised: September 27, 2000 / Accepted: January 30, 2001     

Influence of leaf trichomes on boundary layer conductance and gas‐exchange characteristics in Metrosideros polymorpha (Myrtaceae)

The amount of trichomes on the leaves of Metrosideros polymorpha varies enormously, ranging from 0 to ca 150 g/m² across environmental gradients on the island of Hawaii. Pubescent individuals are abundant in dry areas or on young lava flows, whereas glabrous individuals are abundant in wet areas or on developed soils. To understand the adaptive advantages of pubescent individuals in arid environments, we addressed the following questions: (1) whether leaf trichomes increase the boundary layer resistance to gas diffusion, which in turn reduces the transpiration rate and increases water‐use efficiency (WUE); and (2) whether pubescent individuals have other associated leaf and shoot traits that have adaptive significance in arid environments. We made detailed ecophysiological measurements on M. polymorpha in three populations in habitats that varied in aridity. We found a large allocation of leaf mass to trichomes, up to 33 percent at the arid site, but our analyses showed that trichomes had small effects (1–9%) on gas exchange and negligible effects on WUE, suggesting the trichomes may have roles beyond increasing WUE. However, pubescent individuals did have higher Rubisco amount and a lower leaf‐to‐ sapwood area ratio, which are considered adaptive in arid environments. These results suggest that pubescent individuals of M. polymorpha are indeed adapted to arid environments with changes in a suite of traits. The adaptive significance of the enormous variation in amounts of trichomes remains unclear and may be related to functions other than increasing boundary layer resistance.     

The emetic activity of staphylococcal enterotoxins,SEK, SEL,SEM, SEN and SEO in a small emetic animal model,the house musk shrew

Staphylococcal enterotoxins (SEs) produced by Staphylococcus aureus are the most recognizable causative agents of emetic food poisoning in humans. New types of SEs and SE‐like (SEl) toxins have been reported. Several epidemiological investigations have shown that the SEs and SEl genes, particularly, SEK, SEL, SEM, SEN and SEO genes, are frequently detected in strains isolated from patients with food poisoning. The purpose of the present study was to evaluate the emetic activity of recently identified SEs using a small emetic animal model, the house musk shrew. The emetic activity of these SEs in house musk shrews was evaluated by intraperitoneal administration and emetic responses, including the number of shrews that vomited, emetic frequency and latency of vomiting were documented. It was found that SEs induce emetic responses in these animals. This is the first time to demonstrate that SEK, SEL, SEM, SEN and SEO possess emetic activity in the house musk shrew.     

Rediscovery of the “extinct” blind ground beetle (Coleoptera: Carabidae: Trechinae)

The blind ground beetle Ishikawatrechus intermedius (Coleoptera: Carabidae: Trechinae), called Kadota‐mekurachibigomimushi in Japanese, was first found in 1952 in the cave Ôchi‐dô, Kôchi Prefecture, Japan. However, this cave and a large part of the surrounding hill were destroyed by limestone mining in the 1970s. Because most species of blind ground beetles are restricted to specific caves or cave systems, I. intermedius has been considered endemic to very narrow areas of the cave Ôchi‐dô and the upper hypogean zone around the cave. Therefore, I. intermedius was considered to have become extinct. In this study, during 2011–2015, we collected ten individuals of a blind ground beetle, which appeared to be I. intermedius, from the upper hypogean zone, approximately 0.3–1.0 m below ground, in the southern part of the type locality. A comparison with type specimens indicated that the individuals were indeed I. intermedius. Therefore, we conclude that I. intermedius survives in the upper hypogean zone near the type locality. The results of this study will be useful for quickly establishing conservation measures for threatened or vulnerable species of Trechinae beetles.     

The Effects of Touching and Stroking a Cat on the Inferior Frontal Gyrus in People

Our study evaluated the effects on the prefrontal cortex, especially the inferior frontal gyrus (IFG), of people when touching and stroking a real or soft toy cat, using functional near infrared spectroscopy. Thirty under-graduate students (10 males, 20 females) were recruited and performed three tactile tasks with a real cat and a soft toy cat using their right hand. They also filled in the Self-Assessment Manikin (SAM), to measure their emotional responses, and the NEO-Five Factor Inventory, to assess their personalities. During the tactile interactions with the real cat, the integral values of oxygenated hemoglobin in the left IFG of the females were significantly greater than in the males. The valence scores of the SAM after the real cat-associated tasks in females were significantly higher than after the toy cat-associated tasks. Additionally, the number of times the females stroked the real cat was significantly positively correlated with the activation levels of the left IFG and the valence scores of the SAM. The activation levels of the left IFG in females were also positively correlated with neuroticism when stroking the real cat. Thus, in females, especially those with higher levels of neuroticism, touching/stroking the cat improved their mood. The effects of interacting with a cat may be different between the genders.     

Bioethanol production from steam-exploded rice husk by recombinant <Emphasis Type="Italic">Escherichia coli</Emphasis> KO11

Rice husk is one of the most abundant types of lignocellulosic biomass. Because of its significant amount of sugars, such as cellulose and hemicellulose, it can be used for the production of biofuels such as bioethanol. However, the complex structure of lignocellulosic biomass, consisting of cellulose, hemicellulose and lignin, is resistant to degradation, which limits biomass utilization for ethanol production. The protection of cellulose by lignin contributes to the recalcitrance of lignocelluloses to hydrolysis. Therefore, we conducted steam-explosion treatment as pretreatment of rice husk. However, recombinant Escherichia coli KO11 did not ferment the reducing sugar solution obtained by enzymatic saccharification of steam-exploded rice husk. When the steam-exploded rice husk was washed with hot water to remove inhibitory substances and M9 medium (without glucose) was used as a fermentation medium, E. coli KO11 completely fermented the reducing sugar solution obtained by enzymatic saccharification of hot water washing-treated steam-exploded rice husk to ethanol. We report here the efficient production of bioethanol using steam-exploded rice husk.     

Practical enantioresolution of alcohols with 2-methoxy-2-(1-naphthyl)propionic acid and determination of their absolute configurations by the (1)H NMR anisotropy method.

The enantioresolution of racemic alcohols as esters of 2-methoxy-2-(1-naphthyl)propionic acid (MalphaNP acid 1) and the determination of their absolute configurations on the basis of (1)H NMR anisotropy effect are described. The enantiopure MalphaNP acid (S)-(+)-1 was allowed to react with racemic 2-alkanols and 1-octyn-3-ol, yielding diastereomeric mixtures of esters, which were easily separated by HPLC on silica gel. To determine the absolute configurations of the first-eluted diastereomeric esters by the (1)H NMR anisotropy method, the general scheme was proposed. Separated esters were reduced with LiAlH(4) or hydrolyzed with KOH/EtOH to recover enantiopure alcohols.     

Mechanism of NO-mediated oxidative and nitrative DNA damage in hamsters infected with Opisthorchis viverrini: a model of inflammation-mediated carcinogenesis.

Inflammation mediated by infection is an important factor causing carcinogenesis. Opisthorchis viverrini (OV) infection is a risk factor of cholangiocarcinoma (CHCA), probably through chronic inflammation. Formation of 8-nitroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), and expression of inducible nitric oxide synthase (iNOS) and heme oxygenase-1 (HO-1) were assessed in the liver of hamsters infected with OV. We newly produced specific anti-8-nitroguanine antibody without cross-reaction. Double immunofluorescence staining revealed that 8-oxodG and 8-nitroguanine were formed mainly in the same inflammatory cells and epithelium of bile ducts from day 7 and showed the strongest immunoreactivity on days 21 and 30, respectively. It is noteworthy that 8-oxodG and 8-nitroguanine still remained in epithelium of bile ducts on day 180, although amount of alanine aminotransferase activity returned to normal level. A time course of 8-nitroguanine was associated with iNOS expression. Furthermore, this study demonstrated that HO-1 expression and subsequent iron accumulation may be involved in enhancement of oxidative DNA damage in epithelium of small bile ducts. In conclusion, nitrative and oxidative DNA damage via iNOS expression in hamsters infected with OV may participate in CHCA carcinogenesis.