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31.
Innate and acquired immunity in atherogenesis 总被引:33,自引:0,他引:33
Binder CJ Chang MK Shaw PX Miller YI Hartvigsen K Dewan A Witztum JL 《Nature medicine》2002,8(11):1218-1226
32.
Karpenko IL Jasko MV Andronova VL Ivanov AV Kukhanova MK Galegov GA Skoblov YS 《Nucleosides, nucleotides & nucleic acids》2003,22(3):319-328
Phosphonate derivatives of acyclovir containing phosphorous acid and ethoxycarbonylphosphonic acid residues as well as their isopropyl esters were prepared. They selectively inhibited the herpes simplex virus 1 reproduction in Vero cell culture, the efficacy of esters being 3-4 times higher than that of ACV. The hydrolysis of the synthesized compounds was studied in the PBS buffer and human blood serum. 相似文献
33.
Shimansky YP 《Biological cybernetics》2000,83(4):379-389
The existence and utilization of an internal representation of the controlled object is one of the most important features
of the functioning of neural motor control systems. This study demonstrates that this property already exists at the level
of the spinal motor control system (SMCS), which is capable of generating motor patterns for reflex rhythmic movements, such
as locomotion and scratching, without the aid of the peripheral afferent feedback, but substantially modifies the generated
activity in response to peripheral afferent stimuli. The SMCS is presented as an optimal control system whose optimality requires
that it incorporate an internal model (IM) of the controlled object's dynamics. A novel functional mechanism for the integration
of peripheral sensory signals with the corresponding predictive output from the IM, the summation of information precision
(SIP) is proposed. In contrast to other models in which the correction of the internal representation of the controlled object's
state is based on the calculation of a mismatch between the internal and external information sources, the SIP mechanism merges
the information from these sources in order to optimize the precision of the controlled object's state estimate. It is demonstrated,
based on scratching in decerebrate cats as an example of the spinal control of goal-directed movements, that the results of
computer modeling agree with the experimental observations related to the SMCS's reactions to phasic and tonic peripheral
afferent stimuli. It is also shown that the functional requirements imposed by the mathematical model of the SMCS comply with
the current knowledge about the related properties of spinal neuronal circuitry. The crucial role of the spinal presynaptic
inhibition mechanism in the neuronal implementation of SIP is elucidated. Important differences between the IM and a state
predictor employed for compensating for a neural reflex time delay are discussed.
Received: 8 February 2000 / Accepted: 24 March 2000 相似文献
34.
Yury V. Matsuka Deborah A. Dilts Susan Hoiseth Rasappa Arumugham 《The protein journal》1998,17(7):719-728
An outer membrane PIA protein fromNeisseria gonorrhoeae strain FA19 was expressed inEscherichia coli and refoldedin vitro in the presence of zwitterionic detergent. Its proper folding and subunit organization was confirmed by comparison with the
native counterpart. The unfolding of PIA has been investigated using fluorescence spectroscopy and analytical size-exclusion
chromatography methods. Analysis of the denaturation pathway of the PIA revealed that it forms an unusually labile quaternary
structure. In the presence of 1 M guanidinium chloride (GdmCl) or upon heating up to 50°C, dissociation of the PIA oligomer
was observed resulting in the formation of folded monomeric intermediates. Unfolding of monomers occurs at 80°C or in the
presence of 4.3 M GdmCl, indicating high intrinsic stability toward both GdmCl and elevated temperatures. Both oligomeric
and monomeric forms of PIA exhibited affinity to the hydrophobic probe 1-anilinonaphthalene-8-sulfonic acid (ANS) and bind
withK
d=80 and 130 μM, respectively. Denaturation of the PIA completely abolished affinity to ANS, suggesting that hydrophobicity
is a property of the folded state of the porin. 相似文献
35.
Flexible Electronics: Natural Fiber Welded Electrode Yarns for Knittable Textile Supercapacitors (Adv. Energy Mater. 4/2015)
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36.
37.
38.
Alexander Belyy Irina Tabakova Alexander E. Lang Thomas Jank Yury Belyi Klaus Aktories 《PloS one》2015,10(12)
Clostridium perfringens iota toxin is a binary toxin composed of the enzymatically active component Ia and receptor binding component Ib. Ia is an ADP-ribosyltransferase, which modifies Arg177 of actin. The previously determined crystal structure of the actin-Ia complex suggested involvement of Asp179 of actin in the ADP-ribosylation reaction. To gain more insights into the structural requirements of actin to serve as a substrate for toxin-catalyzed ADP-ribosylation, we engineered Saccharomyces cerevisiae strains, in which wild type actin was replaced by actin variants with substitutions in residues located on the Ia-actin interface. Expression of the actin mutant Arg177Lys resulted in complete resistance towards Ia. Actin mutation of Asp179 did not change Ia-induced ADP-ribosylation and growth inhibition of S. cerevisiae. By contrast, substitution of Glu270 of actin inhibited the toxic action of Ia and the ADP-ribosylation of actin. In vitro transcribed/translated human β-actin confirmed the crucial role of Glu270 in ADP-ribosylation of actin by Ia. 相似文献
39.
Michael Groessl Yury O. Tsybin Christian G. Hartinger Bernhard K. Keppler Paul J. Dyson 《Journal of biological inorganic chemistry》2010,15(5):677-688
The binding of the ruthenium-based anticancer drug candidates KP1019, NAMI-A and RAPTA-T towards different double-stranded
oligonucleotides was probed by electrospray ionisation mass spectrometry and compared with that of the widely used platinum-based
chemotherapeutics cisplatin, carboplatin and oxaliplatin. It was found that the extent of adduct formation decreased in the
following order: cisplatin > oxaliplatin > NAMI-A > RAPTA-T > carboplatin > KP1019. In addition to the characterisation of
the adducts formed with the DNA models, the binding sites of the metallodrugs on the oligonucleotides were elucidated employing
top-down tandem mass spectrometry and were found to be similar for all the metallodrugs studied, irrespective of the sequence
of the oligonucleotide. A strong preference for guanine residues was established. 相似文献
40.
Irina B. Magorivska Rostyslav O. Bilyy Anna M. Havrylyuk Valentyna V. Chop'yak Rostyslav S. Stoika Yury Y. Kit 《Journal of molecular recognition : JMR》2010,23(5):495-502
Novel hydrolytic activity of the anti‐histone H1 antibodies (Ab) toward histone H1 and myelin basic protein (MBP) was shown. Blood serum of ten patients with clinically diagnosed systemic lupus erythematosus (SLE), and nine healthy donors (control) were screened for the anti‐histone H1 antibody‐ and anti‐MBP antibody‐mediated specific proteolytic activity. IgGs were isolated by chromatography on Protein G‐Sepharose, and four of ten SLE patients appeared to possess IgGs that were capable of cleaving both histone H1 and MBP. Such activity was confirmed to be an intrinsic property of the IgG molecule, since it was preserved at gel filtration at alkaline and acidic pH. At the same time, proteolytic activity was absent in the sera‐derived Ab of all healthy donors under control. Anti‐histone IgGs were purified by the affinity chromatography on histone H1‐Sepharose. Their cross‐reactivity toward cationic proteins (histones, lysozyme, and MBP) and their capability of hydrolyzing histone H1 and MBP were detected. However, these IgGs were not cleaving core histones, lysozyme, or albumin. Capability of cleaving histone H1 and MBP was preserved after additional purification of anti‐histone H1 IgGs by the HPLC gel filtration. The protease activity of anti‐histone H1 IgG Ab was inhibited by serine protease inhibitors. Copyright © 2010 John Wiley & Sons, Ltd. 相似文献