全文获取类型
收费全文 | 22557篇 |
免费 | 1994篇 |
国内免费 | 2334篇 |
出版年
2024年 | 31篇 |
2023年 | 193篇 |
2022年 | 426篇 |
2021年 | 726篇 |
2020年 | 611篇 |
2019年 | 699篇 |
2018年 | 703篇 |
2017年 | 592篇 |
2016年 | 792篇 |
2015年 | 1283篇 |
2014年 | 1479篇 |
2013年 | 1638篇 |
2012年 | 2074篇 |
2011年 | 1788篇 |
2010年 | 1275篇 |
2009年 | 1172篇 |
2008年 | 1450篇 |
2007年 | 1317篇 |
2006年 | 1236篇 |
2005年 | 1143篇 |
2004年 | 1046篇 |
2003年 | 956篇 |
2002年 | 803篇 |
2001年 | 557篇 |
2000年 | 468篇 |
1999年 | 431篇 |
1998年 | 279篇 |
1997年 | 214篇 |
1996年 | 196篇 |
1995年 | 175篇 |
1994年 | 152篇 |
1993年 | 112篇 |
1992年 | 140篇 |
1991年 | 109篇 |
1990年 | 91篇 |
1989年 | 89篇 |
1988年 | 59篇 |
1987年 | 70篇 |
1986年 | 53篇 |
1985年 | 28篇 |
1984年 | 18篇 |
1983年 | 35篇 |
1982年 | 16篇 |
1981年 | 17篇 |
1979年 | 17篇 |
1978年 | 14篇 |
1977年 | 13篇 |
1975年 | 10篇 |
1972年 | 12篇 |
1970年 | 11篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
911.
Lian Liu Kwang Seok Ahn Muthu K Shanmugam Hong Wang Hongyuan Shen Frank Arfuso Arunachalam Chinnathambi Sulaiman Ali Alharbi Yung Chang Gautam Sethi Feng Ru Tang 《Journal of cellular biochemistry》2019,120(3):4504-4513
Oleuropein is one of the most abundant phenolic compounds found in olives. Epidemiological studies have indicated that an increasing intake of olive oil can significantly reduce the risk of breast cancer. However, the potential effect(s) of oleuropein on estrogen receptor (ER)-negative breast cancer is not fully understood. This study aims to understand the anticancer effects and underlying mechanism(s) of oleuropein on ER-negative breast cancer cells in vitro. The effect of oleuropein on the viability of breast cancer cell lines was examined by mitochondrial dye-uptake assay, apoptosis by flow cytometric analysis, nuclear factor-κB (NF-κB) activation by DNA binding/reporter assays and protein expression by Western blot analysis. In the present report, thiazolyl blue tetrazolium bromide assay results indicated that oleuropein inhibited the viability of breast cancer cells, and its effects were more pronounced on MDA-MB-231 as compared with MCF-7 cells. It was further found that oleuropein increased the level of reactive oxygen species and also significantly inhibited cellular migration and invasion. In addition, the activation of NF-κB was abrogated as demonstrated by Western blot analysis, NF-κB-DNA binding, and luciferase assays. Overall, the data indicates that oleuropein can induce substantial apoptosis via modulating NF-κB activation cascade in breast cancer cells. 相似文献
912.
913.
914.
Guoxi Li Yinli Zhao Yuanfang Li Yi Chen Wenjiao Jin Guirong Sun Ruili Han Yadong Tian Hong Li Xiangtao Kang 《Journal of cellular biochemistry》2019,120(8):13625-13639
915.
916.
917.
918.
Guo-Hui Huang Xiang-Yuan Cao Yuan-Yuan Li Cheng-Cheng Zhou Lei Li Ke Wang Hong Li Peng Yu Yi Jin Liang Gao 《Journal of cellular biochemistry》2019,120(9):15776-15789
Traumatic brain injury (TBI) is a serious public health problem as well as a leading cause of severe posttraumatic disability. Numerous studies indicate that the differentially expressed genes (DEGs) of neural signaling pathways are strongly correlated with brain injury. To further analyze the roles of the DGEs in the central nervous system, here we systematically investigated TBI on the hippocampus and its injury mechanism at the whole genome level. On the basis of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes Analyses, we revealed that the DEGs were involved in many signaling pathways related to the nervous system, especially neuronal survival-related pathways. Finally, we verified the microarray results and detected the gene expression of neuronal survival-related genes in the hippocampus by using real-time quantitative polymerase chain reaction. With Western blot and axon growth assay, the expression of P2rx3 was upregulated in rats subjected to TBI, and overexpression of P2rx3 promoted neurite growth of NG108 cells. Our results suggested that the DEGs (especially P2rx3) and several signaling pathways might play a pivotal role in TBI. We also provided several targeted genes related to TBI for future investigation. 相似文献
919.
920.
Fei Long Hong Jiang Hongli Yi Lili Su Jian Sun 《Journal of cellular biochemistry》2019,120(3):3294-3305
The impact of particulate matter 2.5 (PM2.5) on the respiratory system is a worldwide concern. However, the mechanisms by which PM2.5 causes disease are still unclear. In this study, we investigated the effect of PM2.5 on autophagy and studied the effect of PM2.5-induced autophagy and 5′-adenosine monophosphate-activated protein kinase (AMPK) on cell proliferation, cell cycle, apoptosis, reactive oxygen species (ROS), and airway inflammation using human bronchial epithelial cells 16HBE140 cells. Results showed that exposure of cells to PM2.5 at a concentration of 100 μg/mL for 24 hours was most effective for inhibiting cell viability. PM2.5 induced cell arrest in the G0/G1 phase and increased mitochondrial membrane potential, ROS, and cell apoptosis with increasing concentration. PM2.5 downregulated cyclin D and matrix metallopeptidase-9 (MMP-9) expression but upregulated tissue inhibitor of metalloproteinases-1 (TIMP-1) expression, significantly promoted interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) production, and enhanced the level and activation of AMPK. The levels of autophagy-related protein 5 (ATG5), Beclin-1, and LC3II/I were significantly increased by PM2.5. The activation of Unc-51-like autophagy activating kinase 1 was significantly inhibited by PM2.5. Moreover, ATG5 knockdown inhibited PM2.5-induced autophagy, ROS, and cell apoptosis significantly. The expression of cyclin D, MMP-9, and TIMP-1 was reversed by ATG5 suppression. PM2.5-induction of IL-6 and TNF-α was significantly inhibited by knockdown of ATG5. Thus, inhibition of autophagy protected the cells from PM2.5-induced injury. PM2.5 induced injury in human bronchial epithelial cells via activation of AMPK-mediated autophagy, suggesting possible therapeutic targets for the treatment of respiratory diseases. 相似文献