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871.
872.
T. L. Yurkshtovich N. V. Golub N. K. Yurkshtovich P. M. Bychkovskii R. I. Kosterova V. A. Alinovskaya 《Applied Biochemistry and Microbiology》2017,53(8):814-822
Gel-forming starch phosphates with a content of acidic phosphate groups ranging from 2.1 to 3.8 mmol/g were obtained in a phosphoric acid-urea system. The rates of starch phosphate degradation in buffer solution in the absence of amylase and in the presence of this enzyme were investigated in vitro. Starch phosphate gels were shown to be prone to enzymatic degradation. However, the rate of biodegradation decreased gradually as the content of phosphate groups increased. The use of microgels with average particle sizes in the range of 7.8–60.1 μm for the production of controlled-release preparations of interferon-alpha 2b has been demonstrated to be possible in principle. 相似文献
873.
Growth and characterization of epithelial cells from normal human uterine ectocervix and endocervix 总被引:3,自引:0,他引:3
M. E. Turyk T. R. Golub N. B. Wood J. L. Hawkins G. D. Wilbanks 《In vitro cellular & developmental biology. Plant》1989,25(6):544-556
Summary The human uterine cervix consists of an endocervical canal lined with a single layer of columnar mucus-secreting cells and
an outer ectocervix covered by a stratified squamous epithelium. We report here the culture of human endocervical epithelial
cells (HEnE) and human ectocervical epithelial cells (HEcE) in serum-free medium (KGM). Both HEnE and HEcE cultures were composed
of keratinocytelike cells which formed desmosomal contacts and stratified in the presence of high concentrations of calcium
ions. Cells with a pleomorphic epithelial morphology were observed in HEnE cultures, but not in HEcE cultures. Keratin 18,
which is characteristic of endocervix in vivo, was detected by indirect immunofluorescent staining in all HEnE cells but was
never detected in cultured HEcE. HEcE expressed keratin 13 which is characteristic of ectocervix in vivo. Although keratin
13 was never detected in primary HEnE cultures, it was expressed in passaged HEnE cultures grown in medium with high concentrations
of calcium and in late passage HEnE cultures. HEnE underwent an average of 15.1 population doublings during serial culture.
Mean colony-forming efficiency during Passages 2 to 3 was 14.7% and mean population doubling time was 17.8 h. HEcE cultures
underwent significantly more population doublings (29.0) than HEnE cultures, whereas colony-forming efficiencies and doubling
times were similar to those determined for HEnE. HEnE and HEcE cells may be useful in developing in vitro models of cervical
squamous metaplasia and for exploring the interactions between target cell differentiation, carcinogens, and papillomaviruses
in the development of cervical neoplasia.
This study was supported in part by the Rush University Committee on Research and by the Lester B. and Francis Knight and
the S. Charles and Marsha Papageorge research funds. 相似文献
874.