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81.
Tissue plasminogen activator was partially purified from the inferior turbinate and nasal polyp, and its biochemical properties were investigated. Similar TPA peak positions were seen in the gel filtration chromatography of both tissues, and the molecular weight was approximately 65,000, which was comparable to TPA of pig heart (55,000-60,000). Activity of TPA from inferior turbinate was higher than that from nasal polyp. TPA from both tissues was completely inhibited by trans-aminomethyl cyclohexane carboxylic acid, dithiothreitol, and diisopropylfluorophosphate and had similar inhibition profiles to TPA from pig heart. All these findings indicate that TPA from both tissues is undoubtedly a plasminogen-activating enzyme and serine-type protease and would be biochemically identical. 相似文献
82.
83.
Redox imbalance in cystine/glutamate transporter-deficient mice 总被引:1,自引:0,他引:1
Sato H Shiiya A Kimata M Maebara K Tamba M Sakakura Y Makino N Sugiyama F Yagami K Moriguchi T Takahashi S Bannai S 《The Journal of biological chemistry》2005,280(45):37423-37429
Cystine/glutamate transporter, designated as system x(-)(c), mediates cystine entry in exchange for intracellular glutamate in mammalian cells. This transporter consists of two protein components, xCT and 4F2 heavy chain, and the former is predicted to mediate the transport activity. This transporter plays a pivotal role for maintaining the intracellular GSH levels and extracellular cystine/cysteine redox balance in cultured cells. To clarify the physiological roles of this transporter in vivo, we generated and characterized mice lacking xCT. The xCT(-/-) mice were healthy in appearance and fertile. However, cystine concentration in plasma was significantly higher in these mice, compared with that in the littermate xCT(-/-) mice, while there was no significant difference in plasma cysteine concentration. Plasma GSH level in xCT(-/-) mice was lower than that in the xCT(-/-) mice. The embryonic fibroblasts derived from xCT(-/-) mice failed to survive in routine culture medium, and 2-mercaptoethanol was required for survival and growth. When 2-mercaptoethanol was removed from the culture medium, cysteine and GSH in these cells dramatically decreased, and cells started to die within 24 h. N-Acetyl cysteine also rescued xCT(-/-)-derived cells and permitted growth. These results demonstrate that system x(-)(c) contributes to maintaining the plasma redox balance in vivo but is dispensable in mammalian development, although it is vitally important to cells in vitro. 相似文献
84.
Cytoplasmic Sequestration of the Polyomavirus Enhancer Binding Protein 2 (PEBP2)/Core Binding Factor α (CBFα) Subunit by the Leukemia-Related PEBP2/CBFβ-SMMHC Fusion Protein Inhibits PEBP2/CBF-Mediated Transactivation
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Yuka Kanno Tomohiko Kanno Chohei Sakakura Suk-Chul Bae Yoshiaki Ito 《Molecular and cellular biology》1998,18(7):4252-4261
85.
Haeyoung Na Ki Woo Kim Yurina Kwack Sung Kyeom Kim Changhoo Chun 《Journal of Plant Biology》2007,50(3):344-350
Anatomical differences between embryogenic and non-embryogenic calli ofPimpinella brachycarpa were investigated by light microscopy and electron microscopy. Initial callus tissue emerged from expiants after 14 d of
culturing. The embryogenie calli (EC) were firm, rather opaque, and light yellow in color. The cells usually formed small,
compact clusters. Nonembryogenic calli (NEC), however, were friable, semitransparent, and yellow or gray. These formed relatively
larger and loosely held clusters. Scanning electron microscopy showed that EC were composed of individual compact and spherical
cells that were rather regular in size and approximately 20 μm long. All were tightly held together and appeared to organize
globular embryos. In contrast, the NEC comprised elongated and loosely held cells that were approximately 50 μm long. Tubular
and u-shaped NEC cells protruded irregularly, and were of varying heights along the cell aggregates. Transmission electron
microscopy of the EC revealed typical eukaryotic cytoplasmic components, including nuclei, mitochondria, and vacuoles in the
cytoplasm enclosed by an electron-transparent cell wall. Based on the numerous ribosomes within the cytoplasm, these cells
appeared to be well-organized and metabolically active. The NEC cells were much larger and more highly vacuolated than those
of the EC. In ultrathin sections, the former seemed to be almost devoid of other cellular contents except for plastids and
nuclei. Furthermore, EC and NEC showed different regeneration capacities in their somatic embryo formation. Most EC produced
hyperhydric somatic embryos, followed by normal somatic embryos; whereas only a few shooted or rooted somatic embryos arose
from the NEC. 相似文献
86.
The distribution of immuno-reactive tenascin in the epithelial-mesenchymal junctional areas of benign and malignant squamous epithelia 总被引:4,自引:0,他引:4
R. Anbazhagan T. Sakakura B. A. Gusterson 《Virchows Archiv. B, Cell pathology including molecular pathology》1990,59(1):59-63
Tenascin is an extra cellular matrix glycoprotein which is distributed in the mesenchyme surrounding various organs during embryogenesis. It has also been demonstrated in some normal adult tissues and in the matrix of human tumours. The present study has been carried out to analyse the distribution of tenascin in non malignant and malignant skin disorders, in squamous cell carcinomas of the head and neck, in squamous cell carcinoma xenografts and in a squamous cell carcinoma cell line grown on collagen gel. Immunohistochemical localisation of tenascin was performed, using a monoclonal antibody specific for tenascin, by the indirect immunoperoxidase method with silver enhancement. Tenascin was heterogeneously distributed in the extra cellular matrix of squamous cell carcinomas and in squamous cell carcinoma xenografts. It was absent in basal cell carcinoma and in the squamous cell carcinoma cell line grown on collagen gel. The distribution of tenascin in squamous cell carcinoma and basal cell carcinoma is discussed in relation to tumour invasion and differentiation. 相似文献
87.
Immunofluorescent localization of tenascin during development of the mouse urogenital sinus: Possible involvement in genital duct morphogenesis 总被引:1,自引:0,他引:1
Hiroyuki Takeda Yasuteru Oike Teruyo Sakakura 《Differentiation; research in biological diversity》1988,39(2):131-138
Tenascin is a compound of the mesenchymal extracellular matrix and has been proposed as a possible mediator in epithelial-mesenchymal interactions, because of its characteristic distribution in tissues during fetal development. In the present study, we have investigated by immunofluorescence the changes in the distribution of tenascin during development of the mouse urogenital sinus, a process in which tissue interactions were found to be essential. Tenascin first appears in dorsal mesenchyme on days 13-15 of gestation, coinciding with morphological changes of the epithelium. During male development, tenascin accumulates in the dorsal mesenchyme around the junction of Wolffian ducts, but not in the ventral mesenchyme, into which prostatic buds (prostate gland anlagen) project from the sinus epithelium. During female development, the mesenchyme that participates in the downgrowth of the vagina (derived from Müllerian ducts) stains intensively for tenascin. In both of these tenascin-positive areas, the epithelium undergoes conspicuous morphogenetic changes. The results suggest that mesenchymal tenascin could be involved in the epithelial morphogenesis of the sinus, especially in the morphogenesis of the genital ducts. 相似文献
88.
Summary Merkel cells in the lower labial mucosa of adult rabbits were studied electron microscopically, 9, 21, 28, and 50 days after resection of the mental nerves. By day 9, nerve fibers were completely retracted from the epithelial layer of the mucosa. On and after day 21, Merkel cells were located not only in the basal layer but also in the prickle or more superficial cell layers. The ultrastructure of the migrating Merkel cells was unchanged, both as to the amount and location of the specific cored granules in the cytoplasm, until the cells reached the granular cell layer. The position of the migrating Merkel cells differed from cell to cell, and migration continued for at least 50 days. A remarkably large number of immature Merkel cells was observed in the basal and suprabasal cell layers of the denervated epithelium even by day 50. Therefore, the possibility of the reproduction of Merkel cells exists. The migrating Merkel cells, as well as the keratinocytes in the same cell layer, had degenerated drastically in the parakeratinized cell layer. This seems to indicate that the Merkel cells belong to the line of keratinocytes. 相似文献
89.
90.
Shingo Nakada Masayoshi Sakakura Hideo Takahashi Suguru Okuda Hajime Tokuda Ichio Shimada 《The Journal of biological chemistry》2009,284(36):24634-24643
Lipoproteins that play critical roles in various cellular functions of Gram-negative bacteria are localized in the cells inner and outer membranes. Lol proteins (LolA, LolB, LolC, LolD, and LolE) are involved in the transportation of outer membrane-directed lipoproteins from the inner to the outer membrane. LolA is a periplasmic chaperone that transports lipoproteins, and LolB is an outer membrane receptor that accepts lipoproteins. To clarify the structural basis for the lipoprotein transfer from LolA to LolB, we examined the interaction between LolA and mLolB, a soluble mutant of LolB, using solution NMR spectroscopy. We determined the interaction mode between LolA and mLolB with conformational changes of LolA. Based upon the observations, we propose that the LolA·LolB complex forms a tunnel-like structure, where the hydrophobic insides of LolA and LolB are connected, which enables lipoproteins to transfer from LolA to LolB.Gram-negative bacteria express lipid-modified proteins, lipoproteins, which are anchored to the cellular membrane via acyl chains attached to N-terminal cysteine residues of the lipoproteins. Putative lipoproteins have been found in various bacteria. For example, Escherichia coli has at least 90 types of lipoproteins (1), and the Lyme disease spirochete Borrelia burgdorferi has 105 putative lipoproteins (2). Although little is known about the functions of the majority of lipoproteins, some of the lipoproteins play essential roles in various cellular functions of Gram-negative bacteria, such as cell surface structure stabilization, cell shape maintenance, substrate transport, cell growth, and cell signaling (3).Lipoproteins are located at three cellular membrane sites; they are the periplasmic side of the inner membrane, the periplasmic side of the outer membrane, and the outside of the outer membrane (4). In E. coli most of the lipoproteins are anchored to the periplasmic side of the outer membrane, whereas others are anchored to that of the inner membrane (1). Therefore, the transportation of the lipoproteins to the outer membrane is essential for E. coli.Five Lol proteins, LolA, LolB, LolC, LolD, and LolE, play central roles in the outer membrane-directed lipoprotein localization. The Lol·CDE complex, which is anchored to the inner membrane, transfers the lipoproteins from the membrane to a soluble monomer periplasmic protein, LolA (182 amino acids) in an ATP-dependent manner (5–7). LolA transports the lipoproteins from the inner membrane through the periplasmic space to the outer membrane and transfers them to an outer membrane lipoprotein, LolB (186 amino acids). LolB is anchored to the membrane by acyl chains attached to its N-terminal cysteine, and it finally inserts the lipoproteins into the outer membrane (8–10).Among the Lol proteins the crystal structures of LolA and LolB have been solved. As for LolB, the soluble mutant of LolB, mLolB, in which the N-terminal cysteine residue was replaced with an alanine residue, was used for the structural analysis. Although LolA and mLolB share only 8% primary sequence identity, their tertiary structures are similar to each other (11). The structures of both LolA and mLolB resemble an open β-barrel with a lid. The convex side of the β-barrel is fully solvent-exposed, whereas the concave side is partly exposed (supplemental Fig. S1).The open β-barrels of LolA and LolB comprise 11 antiparallel β-strands (β1–β11) and an extra β-strand, β12 for LolA and β11′ for LolB. The lid is composed of three α-helices (α1–α3) and is embedded in the concave side of the β-barrel. The concave sides of LolA and LolB contain many hydrophobic residues. Therefore, this concave side of the proteins is speculated to be the binding site for the hydrophobic acyl chains of lipoproteins. Interestingly, one of the crystal structures of LolB accommodated a molecule of polyethylene glycol 2000 monomethyl ether, PEGMME2000, on the hydrophobic surface of the concave side (supplemental Fig. S1).The specific interaction between LolA and LolB is a decisive step in correctly sorting lipoproteins from LolA via LolB to the outer membrane. However, the structural aspects of the interaction, which would clarify how LolA transfers lipoproteins to LolB, remain unknown. To address this issue, we focused on the interaction between LolA and LolB.Here we investigated the interaction of LolA with LolB by NMR spectroscopy. We used LolA with a His6 tag and mLolB, which retain the biological activities similar to those of the wild type protein (8, 12). By exploiting the cross-saturation and paramagnetic relaxation enhancement (PRE)2 techniques, we successfully determined the interfacial residues of LolA and mLolB and the relative orientation of the two molecules in the complex. In addition, we identified the binding sites of an acyl chain analogue, decanoate, on LolA and mLolB. The results obtained from the present study not only explain how LolA might achieve lipoprotein transfer to LolB but also may provide new insights into the structural and functional aspects of other fatty acid-binding proteins. 相似文献