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991.
Francesca ParatiRoberto Altieri Alessandro EspositoArianna Lobianco Milva PepiLorenzo Montesi Tan Nair 《International biodeterioration & biodegradation》2011,65(1):160-163
The production of a substrate containing destoned olive mill solid waste for the cultivation of Agaricus bisporus (Lange) Imbach on an industrial scale was studied. A standard mushroom compost (C) mainly made from straw and poultry manure was compared with the experimental compost (EC) containing the same ingredients as (C) but with added olive mill solid waste (10.6% w/w). Microbial indicators such as counts of heterotrophic microbes and actinomycetes were higher in EC than in C. In addition, compost selectivity as indicated by higher mushroom yield and biological efficiency of EC was higher than that of C. Market quality of the mushrooms produced in both C and EC were comparable. These findings support our work that olive mill solid waste can be used safely in thermal composting process to produce a selective substrate for industrial-scale cultivation of A. bisporus. This study also demonstrates an environmentally sustainable system to manage solid waste from olive oil extraction processes thus overcoming environmental pollution brought about by irrational disposal of the waste on farm lands. 相似文献
992.
Chen SB Tan JH Ou TM Huang SL An LK Luo HB Li D Gu LQ Huang ZS 《Bioorganic & medicinal chemistry letters》2011,21(3):1004-1009
Discovery of potent and selective ligands for telomeric G-quadruplex DNA is a challenging work. Through a combination approach of pharmacophore model construction, model validation, database virtual screening, chemical synthesis and interaction evaluation, we discovered and confirmed triaryl-substituted imidazole TSIZ01 to be a new telomeric G-quadruplex ligand with potent binding and stabilizing activity to G-quadruplex DNA, as well as a 8.7-fold selectivity towards telomeric G-quadruplex DNA over duplex DNA. 相似文献
993.
Chlorophyll a and primary production were studied in northern South China Sea during summer from 2007 to 2008. Microplankton dominated total phytoplankton biomass in the coast, while picoplankton dominated in the offshore. Algae bloom caused by Thalassionema nitzschioides was found at the subsurface of upwelling regions (D2, C2) in 2008, and maximum of phytoplankton abundance reached 1.58 × 106 ind L?1. Integrated primary production ranged from 189.3 to 976.2 mg m?2 d?1 in 2007, and ranged from 652.1 to 6601 mg m?2 d?1 in 2008. PP showed positive relationship with IPP (p < 0.01) and negative relationship with SST (p < 0.05). Coastal upwelling and Pearl River discharge sustained high PP, and played important role in regulating the phytoplankton biomass and production. 相似文献
994.
Chen SY Chen Y Li YP Chen SH Tan JH Ou TM Gu LQ Huang ZS 《Bioorganic & medicinal chemistry》2011,19(18):5596-5604
A series of novel curcumin analogues were designed, synthesized, and evaluated as potential multifunctional agents for the treatment of AD. The in vitro studies showed that these compounds had better inhibitory properties against Aβ aggregation than curcumin. Superior anti-oxidant properties (better than the reference compound Trolox) of these compounds were observed by the oxygen radical absorbance capacity (ORAC) method and a cell-based assay using DCFH-DA as a probe. In addition they were able to chelate metals such as iron and copper and decrease metal-induced Aβ aggregation. The structure-activity relationships were discussed. The results suggested that our curcumin analogues could be selected as multifunctional agents for further investigation of AD treatment. 相似文献
995.
Li Y Tan C Gao C Zhang C Luan X Chen X Liu H Chen Y Jiang Y 《Bioorganic & medicinal chemistry》2011,19(15):4529-4535
Multi-target EGFR, VEGFR-2 and PDGFR inhibitors are highly useful anticancer agents with improved therapeutic efficacies. In this work, we used two virtual screening methods, support vector machines (SVM) and molecular docking, to identify a novel series of benzimidazole derivatives, 2-aryl benzimidazole compounds, as multi-target EGFR, VEGFR-2 and PDGFR inhibitors. 2-Aryl benzimidazole compounds were synthesized and their biological activities against a tumor cell line HepG-2 and specific kinases were evaluated. Among these compounds, compounds 5a and 5e exhibited high cytotoxicity against HepG-2 cells with IC?? values at ~2 μM. Further kinase assay study showed that compound 5a have good EGFR inhibitory activity and moderate VEGFR-2 and PDGFR inhibitory activities, while 5e have moderate EGFR inhibitory activity and slightly weaker VEGFR-2 and PDGFR inhibitory activities. Molecular docking analysis suggested that compound 5a more tightly interacts with EGFR and PDGFR than compound 5e. Our study discovered a novel series of benzimidazole derivatives as multi-target EGFR, VEGFR-2 and PDGFR kinases inhibitors. 相似文献
996.
HCoV-NL63是新近发现的人冠状病毒,对其外膜糖蛋白-棘突蛋白的表达及功能的研究仍有待深入。本研究利用天坛株痘苗病毒载体,克隆构建可表达HCoV-NL63棘突蛋白四个片段(N端棘突蛋白:S1;C端棘突蛋白:S2;受体结合区大片段:RL;受体结合区小片段:RS)的重组痘苗病毒(vJSC1175-S1;vJSC1175-S2;vJSC1175-RL;vJSC1175-RS),酶切测序证实表达载体构建正确,免疫荧光分析(IFA)各重组痘苗病毒中棘突蛋白不同片段的表达与定位,Western-Blot分析表明各种重组蛋白表达正确。分析结果显示:4种重组蛋白均能有效表达,S1、RL及RS蛋白的荧光主要分布在细胞膜上,而S2蛋白的荧光则主要分布于细胞浆,各个片段的分子量大小与文献报道相同,并可进行正确的翻译修饰(糖基化)。本研究首次采用痘苗病毒天坛株载体构建制备了表达HCoV-NL63棘突蛋白不同片段的重组痘苗病毒,为进一步分析人冠状病毒HCoV-NL63棘突蛋白的结构功能及探索其抗原性和免疫原性奠定了基础。 相似文献
997.
Because of the recent observation of the toxic side effects of Gd(III) based MRI contrast agents in patients with impaired renal function, there is strong interest on developing alternative contrast agents for MRI. In this study, macrocyclic Mn(II) chelates were conjugated to nanoglobular carriers, lysine dendrimers with a silsesquioxane core, to synthesize non-Gd(III) based MRI contrast agents. A generation 3 nanoglobular conjugate of Mn(II)-1,4,7-triaazacyclononane-1,4,7-triacetate-GA amide (G3-NOTA-Mn) was also synthesized and evaluated. The per ion T(1) and T(2) relaxivities of G2, G3, G4 nanoglobular Mn(II)-DOTA monoamide conjugates decreased with increasing generation of the carriers. The T(1) relaxivities of G2, G3, and G4 nanoglobular Mn(II)-DOTA conjugates were 3.3, 2.8, and 2.4 mM(-1) s(-1) per Mn(II) chelate at 3 T, respectively. The T(1) relaxivity of G3-NOTA-Mn was 3.80 mM(-1) s(-1) per Mn(II) chelate at 3 T. The nanoglobular macrocyclic Mn(II) chelate conjugates showed good in vivo stability and were readily excreted via renal filtration. The conjugates resulted in much less nonspecific liver enhancement than MnCl(2) and were effective for contrast-enhanced tumor imaging in nude mice bearing MDA-MB-231 breast tumor xenografts at a dose of 0.03 mmol Mn/kg. The nanoglobular macrocyclic Mn(II) chelate conjugates are promising nongadolinium based MRI contrast agents. 相似文献
998.
RICE MORPHOLOGY DETERMINANT encodes the type II formin FH5 and regulates rice morphogenesis 总被引:1,自引:0,他引:1
Zhang Z Zhang Y Tan H Wang Y Li G Liang W Yuan Z Hu J Ren H Zhang D 《The Plant cell》2011,23(2):681-700
Multicellular organisms contain a large number of formins; however, their physiological roles in plants remain poorly understood. Here, we reveal that formin homology 5 (FH5), a type II formin mutated in rice morphology determinant (rmd), plays a crucial role in determining rice (Oryza sativa) morphology. FH5/RMD encodes a formin-like protein consisting of an N-terminal phosphatase tensin (PTEN)-like domain, an FH1 domain, and an FH2 domain. The rmd mutants display a bending growth pattern in seedlings, are stunted as adult plants, and have aberrant inflorescence (panicle) and seed shape. Cytological analysis showed that rmd mutants have severe cell elongation defects and abnormal microtubule and microfilament arrays. FH5/RMD is ubiquitously expressed in rice tissues, and its protein localization to the chloroplast surface is mediated by the PTEN domain. Biochemical assays demonstrated that recombinant FH5 protein can nucleate actin polymerization from monomeric G-actin or actin/profilin complexes, cap the barbed end of actin filaments, and bundle actin filaments in vitro. Moreover, FH5 can directly bind to and bundle microtubules through its FH2 domain in vitro. Our findings suggest that the rice formin protein FH5 plays a critical role in determining plant morphology by regulating actin dynamics and proper spatial organization of microtubules and microfilaments. 相似文献
999.
The interactions of complex [Ru(bpy)(2)(hnip)](2+) (1) {bpy?=?2,2'-bipyridine, hnip?=?2-(2-hydroxy-1-naphthyl)imidazo[4,5-f][1,10]phenanthroline} with calf thymus DNA and yeast tRNA were investigated by UV-vis spectroscopy, fluorescence spectroscopy, viscosity, equilibrium dialysis, and circular dichroism. In addition, the antitumor activities of complex 1 were evaluated with MTT method. These results indicate that the structures of DNA and RNA have significant effects on the binding behaviors of complex 1. Further, complex 1 demonstrates different antitumor activities against selected cancer cell lines in vitro. 相似文献
1000.
Hum Yan Chai Lai Khin Wee Tan Tian Swee Sh-Hussain Salleh Lim Yee Chea 《Biomedical engineering online》2011,10(1):1-22