Morphological variations of Caragana microphylla populations in the Xilingol steppe and their relationship with environmental factors

The morphological variations of branches, leaves, flowers, fruits and seeds of Caragana microphylla and their correlations with environmental factors were analyzed based on five natural populations in the Xilingol steppe, Inner Mongolia, China. Nested variation analysis showed that there were significant differences in all of the 28 morphological characters within and among populations in 16 morphological characters, and the variations within populations were much greater than those among populations in most morphological characters. Cluster analysis showed that the five populations of C. microphylla could be divided into two groups in morphological characters, and there was significant positive correlation between the divergence of morphological characters matrix and geographical distance matrix. Principal component analysis showed that leaflet length, area of a compound leaf, fresh-mass of a compound leaf, and shoot fresh-mass were main morphological characters that varied among C. microphylla populations. Spearman correlation analysis showed that there were significant correlations between some morphological characters and soil factors. Soil N:P ratio, available N content, total N content, organic matter content and soil water content were main factors that were associated with morphological characters of C. microphylla.     

mRNA的序列、结构以及翻译速率与蛋白质结构的关系

mRNA所包含的核苷酸序列通过三联体密码子决定了蛋白质的氨基酸序列。但是, 由于对氨基酸同义密码使用频率上的差异, 密码子与反密码子相互作用效率上的不同, 以及密码子上下文关系和mRNA 不同区域二级结构上的差异, 造成了核糖体对mRNA 不同区域翻译速度上的差异, 加之共翻译折叠的作用, 使得mRNA 的序列和结构影响着蛋白质空间结构的形成。     

南亚热带河流底栖硅藻β多样性及其对空间距离与环境梯度的响应——基于广义非相似性模拟的分析

β多样性指不同生境间群落物种组成的差异,其空间格局及影响因素是生物多样性维持研究的重要内容。以典型的南亚热带中小型河流—广州流溪河为对象,在对底栖硅藻进行季节调查的基础上,采用Baselga对β多样性的分解框架,基于S?rensen相异性系数将底栖硅藻的β多样性分解为周转和嵌套两个组分,运用广义非相似性模拟(Generalized Dissimilarity Modelling, GDM)分析了空间与环境因子对β多样性及其组分的影响。结果表明：底栖硅藻β多样性、物种周转和嵌套组分无明显季节差异,物种周转是流溪河底栖硅藻β多样性的主要组分(>75%);环境与空间过程共同影响流溪河底栖硅藻β多样性和物种周转组分格局,但环境选择是主要的驱动因子;与枯水期相比,丰水期的空间因素对β多样性和物种周转组分的影响程度降低。作为一种非线性距离回归方法,GDM能较好地识别底栖硅藻β多样性及其组分对环境梯度和空间距离的响应。     

Dynamic genome plasticity during unisexual reproduction in the human fungal pathogen Cryptococcus deneoformans

Genome copy number variation occurs during each mitotic and meiotic cycle and it is crucial for organisms to maintain their natural ploidy. Defects in ploidy transitions can lead to chromosome instability, which is a hallmark of cancer. Ploidy in the haploid human fungal pathogen Cryptococcus neoformans is exquisitely orchestrated and ranges from haploid to polyploid during sexual development and under various environmental and host conditions. However, the mechanisms controlling these ploidy transitions are largely unknown. During C. deneoformans (formerly C. neoformans var. neoformans, serotype D) unisexual reproduction, ploidy increases prior to the onset of meiosis, can be independent from cell-cell fusion and nuclear fusion, and likely occurs through an endoreplication pathway. To elucidate the molecular mechanisms underlying this ploidy transition, we identified twenty cell cycle-regulating genes encoding cyclins, cyclin-dependent kinases (CDK), and CDK regulators. We characterized four cyclin genes and two CDK regulator genes that were differentially expressed during unisexual reproduction and contributed to diploidization. To detect ploidy transition events, we generated a ploidy reporter, called NURAT, which can detect copy number increases via double selection for nourseothricin-resistant, uracil-prototrophic cells. Utilizing this ploidy reporter, we showed that ploidy transition from haploid to diploid can be detected during the early phases of unisexual reproduction. Interestingly, selection for the NURAT reporter revealed several instances of segmental aneuploidy of multiple chromosomes, which conferred azole resistance in some isolates. These findings provide further evidence of ploidy plasticity in fungi with significant biological and public health implications.     

Somatic Embryogenesis in Cell Suspension Culture of Saposhnikovia divaricata

Calli of Saposhnikovia divaricata (Turcz.) Schischk. were induced from the roots of test tube seedlings cultured on MS medium containing 0.5 mg/L 2,4-D. Cell suspension was established by shaking the caul in liquid medium of the same components supplemented with 10% coconut milk. After the formation of embryogenic clusters, 2,4-D was omitted promoting the transformation of the embryogenic clusters to somatic embryos. Micro and submicroscopic: structural changes during the single cell to globular embryonic stage were observed. It was noticed that cortical endoplasmic reticulum appeared in the cells at the stage of embryogenic clump formation but was absent in other stages. Perhaps this was related to the metabolic specification leading to embryo formation. Spherosomes were observed of embryogenesis but remarkably increased in number at proglobular embryo stage. Meanwhile, the central electric dense matrix became progressively smaller and paler, while the outer part became enlarged and more transparent. This implied that the spherosomes took part in the storage of proteins or lipidproteins in the early stages of embryogenesis and transformation to lipid dror)s when the proteins were exhausted in the development of embryo, vacuolar protein bodies could be risualized in many cells in the proglobular embryo stage. This together with the existance and changes of spherosomes was similar to that observed in Peucedanum terebmthaceum. Further studies are meritted to approach, whether these are general phenomena in Umbelliferae. This work also revealed that the aggregation of single cells in suspension culture stimulated the initiation of embryogenesis.     

Urinary 8-isoprostaglandin F(2alpha) level in Behçet's disease

Although its etiology remains unknown, the increased production of reactive oxygen species in Beh?et's disease (BD) have been reported. Furthermore, it has been suggested that vascular and endothelial tissue damage seen in BD is related to elevated reactive oxygen species generated by activated neutrophils from BD patients. To investigate the formation of lipid peroxidation in BD patients in vivo, urinary level of 8-isoprostaglandin F(2alpha) was quantitated by enzyme immunoassay after solid phase extraction in different clinical forms of BD patients. There was no difference in urinary level of 8-isoprostaglandin F(2alpha) between BD patient and healthy control group. There was also no difference in urinary levels of 8-isoprostaglandin F(2alpha) in subgroup analyses of BD patients, i.e. in mucocutaneous and vascular type BD patients; active and inactive BD patients. Contrary to the findings in literature, we found no difference in urinary level of 8-isoprostaglandin F(2alpha) between patients with systemic lupus erythematosus and healthy control group. These findings show no increase in lipid peroxidation despite the augmented formation of reactive oxygen species in BD patients. It may be interesting to assess formation of urinary level of 8-isoprostaglandin F(2alpha) in BD patients who do not take any medication.     

Priming integrin α5 promotes human dental pulp stem cells odontogenic differentiation due to extracellular matrix deposition and amplified extracellular matrix-receptor activity

Our previous study showed that knocking down integrin α5 (ITGA5) expression by using a lentiviral vector in human dental pulp stem cells (DPSCs) led to weakening proliferation and migration capacity while enhanced odontogenic differentiation. To seek for possible clinical application, we investigated the effect of the ITGA5 priming synthetic cyclic peptide (SCP; GA-CRRETAWAC-GA) on proliferation, migration, and the odontogenic differentiation of DPSCs. Remarkably, the involved mechanism was explored by isobaric tag for relative and absolute quantitation proteomic technique, and the in vivo effect of ITGA5 was investigated by nude mice subcutaneous transplantation of cell and hydroxyapatite/β-tricalcium phosphate complex. Results showed that SCP weakened the proliferation and migration capacity while enhanced odontogenic differentiation of DPSCs as lentivirus. The phosphorylation of FAK, PI3K/AKT, and MEK1/2/ERK1/2, along with IGF2/IGFBP2 and Wnt/β-catenin signaling pathway play an important role in this process. Proteomic Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed the key role of extracellular matrix (ECM) and ECM-receptor activity pathway were involved. ECM constituents, secreted protein acidic and cysteine-rich (SPARC), lumican, vitronectin, prolargin, decorin, collagen type VI α1 chain (COL6A1), COL6A2, COL14A1, and COL5A1 were upregulated in the ITGA5-silenced group. Inhibited expression of ITGA5 in DPSCs increased osteoid tissue formation and stronger related genes expression in vivo. In conclusion, the ITGA5 priming peptide could promote DPSCs odontogenic differentiation as lentivirus. Proteomics and bioinformatic analysis revealed that this may be due to the deposition of ECM and amplified ECM-receptor activity, which could fuel the application process of utilizing priming ITGA5 on dental clinical practice.     

Dynamics and stability of polymorphic human telomeric G-quadruplex under tension

As critical DNA structures capping the human chromosome ends, the stability and structural polymorphism of human telomeric G-quadruplex (G4) have drawn increasing attention in recent years. This work characterizes the equilibrium transitions of single-molecule telomeric G4 at physiological K⁺ concentration. We report three folded states of telomeric G4 with markedly different lifetime and mechanical stability. Our results show that the kinetically favored folding pathway is through a short-lived intermediate state to a longer-lived state. By examining the force dependence of transition rates, the force-dependent transition free energy landscape for this pathway is determined. In addition, an ultra-long-lived form of telomeric G4 structure with a much stronger mechanical stability is identified.     

The Influence of Intersections on Fuel Consumption in Urban Arterial Road Traffic: A Single Vehicle Test in Harbin,China

The calculating method for fuel consumption (FC) was put forward and calibrated and the characteristics of the fuel consumption on intersections were analyzed based on 18 groups of vehicular operating data which were acquired from the test experiment of a single vehicle along the urban arterial roads in Harbin, China. The results obtained show that 50.36% of the fuel consumption for the test vehicle was used at the area of intersections compared with 28.9% of the influencing distance and 68.5% of the running time; and 78.4% of them was burnt at the stages of acceleration and idling. Meanwhile, the type (c) of the vehicular operating status was illustrated to be the worst way of reducing fuel consumption, the causes were analyzed and four improvement strategies were put forward.