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691.
Objectives: Technical limitations and evolution of therapeutic applications for cell culture-derived products have accelerated elimination of animal-derived constituents from such products to minimize inadvertent introduction of microbial contaminants, such as fungi, bacteria or viruses. The study described here was conducted to investigate the proliferative effect of vegetable peptone on adult stem cells in the absence of serum, and its possible mechanisms of action.
Materials and methods: Cell viability and proliferation were determined using the MTT assay and Click-iT™ EdU flow cytometry, respectively. In addition, changes in expression of cytokine genes were analysed using MILLIPLEX™ human cytokine enzyme-linked immunosorbent assay kit.
Results: Viability of cord blood-derived mesenchymal stem cells (CB-MSC) and adipose tissue-derived stem cells (ADSC) increased significantly when treated with the peptone. In addition, median value of the group treated with peptone shifted to the right when compared to the untreated control group. Furthermore, quantitative analysis of the cytokines revealed that production of vascular endothelial growth factor (VEGF), transforming growth factor-beta1 (TGF-β1), and interleukin-6 (IL-6) increased significantly in response to treatment with our vegetable peptone in both CB-MSCs and ADSCs.
Conclusions: Our findings revealed that the vegetable peptone promotes proliferation of CB-MSCs and ADSCs. In addition, results of this study suggest that induction of stem cell proliferation by vegetable peptone is likely to be related to its induction of VEGF, TGF-β1, and IL-6 expression. 相似文献
Materials and methods: Cell viability and proliferation were determined using the MTT assay and Click-iT™ EdU flow cytometry, respectively. In addition, changes in expression of cytokine genes were analysed using MILLIPLEX™ human cytokine enzyme-linked immunosorbent assay kit.
Results: Viability of cord blood-derived mesenchymal stem cells (CB-MSC) and adipose tissue-derived stem cells (ADSC) increased significantly when treated with the peptone. In addition, median value of the group treated with peptone shifted to the right when compared to the untreated control group. Furthermore, quantitative analysis of the cytokines revealed that production of vascular endothelial growth factor (VEGF), transforming growth factor-beta1 (TGF-β1), and interleukin-6 (IL-6) increased significantly in response to treatment with our vegetable peptone in both CB-MSCs and ADSCs.
Conclusions: Our findings revealed that the vegetable peptone promotes proliferation of CB-MSCs and ADSCs. In addition, results of this study suggest that induction of stem cell proliferation by vegetable peptone is likely to be related to its induction of VEGF, TGF-β1, and IL-6 expression. 相似文献
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Yong Chan Kim Hye Won Jeong Dong-Min Kim Kyungmin Huh Sang-Ho Choi Hee Young Lee Yunjung Jung Yeol Jung Seong Eun Jin Kim Young Hwa Choi Jung Yeon Heo 《PLoS neglected tropical diseases》2021,15(6)
BackgroundIn South Korea, the number of Q fever cases has rapidly increased since 2015. Therefore, this study aimed to characterize the epidemiological and clinical features of Q fever in South Korea between 2011 and 2017.Methods/Principal findingsWe analyzed the epidemiological investigations and reviewed the medical records from all hospitals that had reported at least one case of Q fever from 2011 to 2017. We also conducted an online survey to investigate physicians’ awareness regarding how to appropriately diagnose and manage Q fever. The nationwide incidence rate of Q fever was annually 0.07 cases per 100,000 persons. However, there has been a sharp increase in its incidence, reaching up to 0.19 cases per 100,000 persons in 2017. Q fever sporadically occurred across the country, with the highest incidences in Chungbuk (0.53 cases per 100,000 persons per year) and Chungnam (0.27 cases per 100,000 persons per year) areas. Patients with acute Q fever primarily presented with mild illnesses such as hepatitis (64.5%) and isolated febrile illness (24.0%), whereas those with chronic Q fever were likely to undergo surgery (41.2%) and had a high mortality rate (23.5%). Follow-up for 6 months after acute Q fever was performed by 24.0% of the physician respondents, and only 22.3% of them reported that clinical and serological evaluations were required after acute Q fever diagnosis.ConclusionsQ fever is becoming an endemic disease in the midwestern area of South Korea. Given the clinical severity and mortality of chronic Q fever, physicians should be made aware of appropriate diagnosis and management strategies for Q fever. 相似文献
694.
The feeding habits of Helicolenus hilgendorfii were studied using 93 specimens (35–135 mm in standard length) collected from the southeastern coast of Korea during the summer season (June–August) from 2005 to 2007. Helicolenus hilgendorfii is a carnivore that mainly consumes shrimps and fishes. Its diet also includes small quantities of mysids, amphipods, euphausiids, copepods, and isopods. Our graphical method for feeding habits revealed that H. hilgendorfii is an opportunistic and specialized predator on shrimps, especially Crangon hakodatei, although H. hilgendorfii showed an ontogenetic dietary shift, the proportion of shrimp decreasing and the consumption of fishes gradually increasing with the increase of fish size. 相似文献
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696.
Length–weight and length–length relationships for six commercial fishes from southern Korean waters
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Presented are the relationships between total length and weight, and between total length and standard length for six commercial fishes collected twice a month from local fish markets in southern Korea between 2005–2006 for Acanthopagrus schlegelii (Bleeker, 1854), Hexagrammos agrammus (Temminck & Schlegel, 1843) and Hexagrammos otakii (Jordan & Starks, 1895), and monthly in 2004 for Dentex tumifrons (Temminck & Schlegel, 1843), Doederleinia berycoides (Hilgendorf, 1879) and Scomberomorus niphonius (Cuvier, 1832). The LWR for D. tumifrons is estimated for the first time, and a new maximum length was recorded for S. niphonius. All total length and weight relationships were significant (all r2 > .953). The values of exponent b, estimated using simple linear least squares of log‐transformed weight and length data, ranged from 2.945 to 3.317. 相似文献
697.
Ho Yul Choo Harry K. Kaya Jin Huh Dong Woon Lee Hyeong Hwan Kim Sang Myeong Lee Young Moo Choo 《BioControl》2002,47(2):177-192
Three species of entomopathogenicnematodes, a combination of two nematodespecies, an entomopathogenic fungal species,and a combination of a nematode and fungalspecies were evaluated against the white grubsEctinohoplia rufipes and Exomalaorientalis (Coleoptera: Scarabaeidae) in the field. The nematodes were acommercial formulation of Steinernemacarpocapsae (BioSafe) and S. glaseri from Dongrae and from Hanrim, and Heterorhabditis bacteriophora from Hamyang,Republic of Korea. The entomopathogenic funguswas Beauveria brongniartii, produced onSabouraud maltose agar plus 1% yeast (SMAY),rice bran, or compost. The combinationtreatment was S. carpocapsae with H.bacteriophora or B. brongniartii. Fieldapplications were made in August or Septemberagainst third instars at a golf course infestedwith E. rufipes in Gyeongnam Province in1991, and one in Pusan with E. orientalisin 1992 and 1993. In 1991, a significantreduction of 70.2 to 79.4% of E. rufipeslarvae was observed in the nematode, fungal andchemical (fenitrothion) treatments comparedwith a 15.7% reduction in the control. In1992, the E. orientalis larval populationwas reduced between 62.7 and 82.8% in thetreatments compared to 10.7% in the control.In 1993, larval reductions in plots treatedwith nematodes (78.3 to 97%) and B.brongniartii propagated on rice bran (84.5%)were significantly better than in plots treatedwith B. brongniartii propagated on SMAY(63.6%) or compost (59.6%). Combining twonematode species did not enhance the efficacycompared to treatments with one nematodespecies alone, but combining S.carpocapsae with B. brongniartiiproduced on SMAY resulted in a significantincrease in grub mortality over the applicationof the fungus alone produced on SMAY orcompost. The high efficacy of the nematode andmost fungal treatments was attributed to theclose proximity of the white grubs to the soilsurface which allowed for excellentpathogen-host contact and to favorable soiltemperatures, sandy soil, post irrigationapplication and/or rain and a minimal thatchlayer in the turfgrass. 相似文献
698.
699.
S H Lee J Y Chai S T Hong W M Sohn S Huh E H Cheong S B Kang 《The Korean journal of parasitology》1989,27(3):213-216
Seven cases of Diphyllobothrium latum infection were proved by collection of worms after praziquantel treatment between October, 1986 and July, 1987. The patients were all males aged 20-44 years residing in Seoul or Ulungdo, Kyungpook Province. All of them had the history of eating several kinds of raw marine fishes, and they had never been to abroad. One of them experienced abdominal pain and 6 experienced natural discharge of a chain of worm segments, but none revealed any sign of anemia. Total 12 worms (1-3/patient) were collected after praziquantel treatment. The worms were 85-423 cm in length, and revealed the characteristic rosette-shape uterus in their gravid proglottides. The average egg size varied 61.0-65.3 x 41.7-46.1 microns. The eggs were yellowish-brown, and ovoid to elliptical. Including the present 7 cases, the total number of human D. latum infections proven by worms in Korea becomes 28 cases. 相似文献
700.