全文获取类型
收费全文 | 673篇 |
免费 | 31篇 |
专业分类
704篇 |
出版年
2023年 | 1篇 |
2022年 | 11篇 |
2021年 | 19篇 |
2020年 | 5篇 |
2019年 | 4篇 |
2018年 | 7篇 |
2017年 | 7篇 |
2016年 | 10篇 |
2015年 | 42篇 |
2014年 | 45篇 |
2013年 | 25篇 |
2012年 | 40篇 |
2011年 | 49篇 |
2010年 | 23篇 |
2009年 | 25篇 |
2008年 | 40篇 |
2007年 | 26篇 |
2006年 | 38篇 |
2005年 | 39篇 |
2004年 | 46篇 |
2003年 | 32篇 |
2002年 | 31篇 |
2001年 | 27篇 |
2000年 | 29篇 |
1999年 | 28篇 |
1998年 | 7篇 |
1997年 | 4篇 |
1996年 | 5篇 |
1995年 | 4篇 |
1994年 | 1篇 |
1993年 | 2篇 |
1992年 | 3篇 |
1991年 | 4篇 |
1990年 | 6篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1982年 | 3篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1972年 | 2篇 |
1970年 | 2篇 |
排序方式: 共有704条查询结果,搜索用时 15 毫秒
681.
Samples of lambdaphage DNA exposed to short pulses of microwave irradiation were subjected to restriction fragmentation by Eco RI and Bam HI. Eco RI digests of microwaved DNA samples yielded three additional fragments ranging in base pair lengths between 24,226 and 7,421 besides the six expected fragments. While Bam HI digests of the microwaved samples did not yield any additional fragments, mobilities of the Bam HI fragments from the microwaved DNA samples were slower and the bands were broader in comparison to those from native samples. We attribute these altered restriction patterns to the conformational anomolies in DNA resulting from single strand breaks and localized strand separations induced by microwave irradiation. 相似文献
682.
Chiral analogues of acetylcholine and AF64A were prepared from I-glutamic acid via the central intermediate, (S)-3-acetoxypiperidine. 相似文献
683.
J R Smith H F Flanigin D W King B B Gallagher A M Murro G L Holmes K Huh 《Applied neurophysiology》1987,50(1-6):380-385
Chronically implanted depth and subdural electrodes have both been shown to be satisfactory means of localizing epileptogenic foci. Utilizing bilateral mesial temporal depth electrodes, we have localized mesial temporal foci in a large percentage of patients. Depth electrode investigation of a more limited number of patients with suspected extramesial temporal foci has not been as reliable in giving localizing information. We have more recently used subdural electrodes to investigate this latter category of patients, and preliminary findings suggest that this technique may be of localizing value in several subcategories. 相似文献
684.
Ki Ju Choi Mi Jeong Kim A Reum Je Sangmi Jun Chulhyun Lee Eunji Lee Mijung Jo Yang Hoon Huh Hee-Seok Kweon 《Journal of biosciences》2014,39(1):97-105
Alzheimer’s disease (AD) is a progressive neurodegenerative disorder. The deterioration of subcellular organelles, including the mitochondria, is another major ultrastructural characteristic of AD pathogenesis, in addition to amyloid plaque deposition. However, the three-dimensional (3-D) study of mitochondrial structural alteration in AD remains poorly understood. Therefore, ultrastructural analysis, 3-D electron tomography, and immunogold electron microscopy were performed in the present study to clarify the abnormal structural alterations in mitochondria caused by the progression of AD in APP/PSEN1 transgenic mice, expressing human amyloid precursor protein, as a model for AD. Amyloid β (Aβ) plaques accumulated and dystrophic neurites (DN) developed in the hippocampus of transgenic AD mouse brains. We also identified the loss of peroxiredoxin 3, an endogenous cytoprotective antioxidant enzyme and the accumulation of Aβ in the hippocampal mitochondria of transgenic mice, which differs from those in age-matched wild-type mice. The mitochondria in Aβ plaque-detected regions were severely disrupted, and the patterns of ultrastructural abnormalities were classified into three groups: disappearance of cristae, swelling of cristae, and bulging of the outer membrane. These results demonstrated that morpho-functional alterations of mitochondria and AD progression are closely associated and may be beneficial in investigating the function of mitochondria in AD pathogenesis. 相似文献
685.
686.
687.
688.
689.
Seo Jin Lee Alla Sreenivasa Rao Youn Ho Shin Hyung-Joo Chung Youngbuhm Huh Kyo Han Ahn Junyang Jung 《Journal of molecular histology》2013,44(2):241-247
In in vitro and in vivo systems, understanding localization and the functional role of ATP is essential, but effective methods to monitor ATP in cells and tissues are limited. Although quinacrine dihydrochloride is a well-known fluorescent dye used to detect ATP, it is limited in its use because it shows non-specific nuclear staining both in vitro and in vivo. A commercial luciferin-luciferase bioluminescence assay has also been used to detect ATP, but it can not be easily used in vivo. Thus, to effectively monitor ATP in vivo, we employed a novel two-photon ATP fluorescent probe, acedan-based Zn(DPA). Using the acedan-based Zn(DPA) probe, we show that this probe produces high quality images of ATP in lung, spleen, liver and spinal cord tissues. 相似文献
690.