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91.
Based on the survey of community plots, a quantitative analysis of endangered Acanthopanax senti-cosus communities in Dongling Mountain was performed with two way indicator species analysis (TWINSPAN), detrended correspondence analysis (DCA) and canonical correspondence analysis (CCA). The communities of A. senticosus were classified into 9 types by TWINSPAN and the results were validated by DCA. On the DCA graph, the first axis reflected the gradient of altitude and the second axis reflected the aspect and slope. Most of A. senticosus were distributed in the thick forests at a high altitude with little light. With the exception of being a dominant species of shrub layers in a few communities, A. senticosus has a relatively scarce distribution. In accordance with DCA, the results of CCA also show the trend that the distribution of A. senticosus communities varied along with the gradient change of environmental factors. Altitude and light are the main factors affecting A. senticosus growth. 相似文献
92.
Min Lin Ying Zhao ShuQi Wang Ming Liu ZhenFeng Duan YongMei Chen Fei Li Feng Xu TianJian Lu 《Biotechnology advances》2012
Lanthanide (Ln)-doped upconversion nanoparticles (UCNPs) with appropriate surface modification can be used for a wide range of biomedical applications such as bio-detection, cancer therapy, bio-labeling, fluorescence imaging, magnetic resonance imaging and drug delivery. The upconversion phenomenon exhibited by Ln-doped UCNPs renders them tremendous advantages in biological applications over other types of fluorescent materials (e.g., organic dyes, fluorescent proteins, gold nanoparticles, quantum dots, and luminescent transition metal complexes) for: (i) enhanced tissue penetration depths achieved by near-infrared (NIR) excitation; (ii) improved stability against photobleaching, photoblinking and photochemical degradation; (iii) non-photodamaging to DNA/RNA due to lower excitation light energy; (iv) lower cytotoxicity; and (v) higher detection sensitivity. Ln-doped UCNPs are therefore attracting increasing attentions in recent years. In this review, we present recent advances in the synthesis of Ln-doped UCNPs and their surface modification, as well as their emerging applications in biomedicine. The future prospects of Ln-doped UCNPs for biomedical applications are also discussed. 相似文献
93.
Previously we found that the organic components in scallop shell promote lipolysis in differentiated 3T3-L1 and C3H10T1/2 adipocyte cells, and that incorporating scallop shell powder into the diet of rats reduced the amount of white adipose tissue. In this study, we used RT-PCR to investigate the effect of ingesting scallop shell powder on the gene expression profile of uncoupling proteins (UCPs) regulating energy metabolism in rats.Feeding of scallop shell powder increased mRNA levels of UCP1 and UCP2 in white adipose tissue. By contrast, scallop shell powder had no effect on the expression of UCP1 in brown adipose tissue, although the expression level of UCP2 mRNA decreased significantly. These results suggest that feeding scallop shell powder increases gene expression of UCP1 that may regulate energy metabolism in white adipose tissue, resulting in the observed reduction in weight of white adipose tissue. 相似文献
94.
95.
家蚕线粒体ND2、COⅠ和若干tRNA基因的克隆及序列分析 总被引:5,自引:0,他引:5
克隆并测定了家蚕(Bombyx mori)线粒体基因组3468bp的EcoRⅠ和HindⅢ双酶一段序列,根据序列同源性比较,该DNA片段包括3个蛋白质编码基因:ND2基因、COⅠ基因和COⅡ基因5′端399bp的序列,以及6个tRNA基因和一个尚待确定的tRNA^Met基因。家蚕与果蝇的ND2基因序列同源性约69.7%,COⅠ基因的同源性约83.8%,COⅡ基因5′端的同源性约80%,这表明细胞色素氧化酶基因在物种间比烟酰胺腺漂呤二核苷酸脱氢酶基因保守,6个推定的tRNA基因序列与果蝇相应tRNA基因序列差异较大,另外,除tRNA^Chn基因的二级结构相似外,其它tRNA基因的二级结构与果蝇相应tRNA基因的二级结构也有较大差异。 相似文献
96.
目的:研究条斑紫菜多糖体外抗肿瘤的生物学活性。方法:应用生化技术分离和纯化条斑紫菜多糖,获得条斑紫菜多糖的2个组分,分别为PY-D1和PY-D2;在体外培养条件下分别用不同浓度的条斑紫菜多糖PY-D2处理4种人肿瘤细胞,通过MTT法观察条斑紫菜多糖PY-D2对4种人肿瘤细胞生长的影响;采用流式细胞仪检测肿瘤细胞的细胞周期变化。结果:条斑紫菜多糖PY-D2诱导HO-8910、MCF-7、K562和7721肿瘤细胞72h后,对其生长有明显的抑制作用,呈剂量依赖效应,500mg/LPY-D2的抑制率分别为21.2%、23.6%、19.8%和21%(P<0.001)。流式细胞仪检测表明PY-D2可以阻滞肿瘤细胞的细胞周期于G0/G1期或G2/M期。结论:条斑紫菜多糖PY-D2具有抑制肿瘤细胞HO-8910、MCF-7、K562和7721生长的作用,其有关的分子生物学作用机理值得进一步研究。 相似文献
97.
98.
Tet1 and Tet2 regulate 5-hydroxymethylcytosine production and cell lineage specification in mouse embryonic stem cells 总被引:2,自引:0,他引:2
Koh KP Yabuuchi A Rao S Huang Y Cunniff K Nardone J Laiho A Tahiliani M Sommer CA Mostoslavsky G Lahesmaa R Orkin SH Rodig SJ Daley GQ Rao A 《Cell Stem Cell》2011,8(2):200-213
TET family enzymes convert 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) in DNA. Here, we show that Tet1 and Tet2 are Oct4-regulated enzymes that together sustain 5hmC in mouse embryonic stem cells (ESCs) and are induced concomitantly with 5hmC during reprogramming of fibroblasts to induced pluripotent stem cells. ESCs depleted of Tet1 by RNAi show diminished expression of the Nodal antagonist Lefty1 and display hyperactive Nodal signaling and skewed differentiation into the endoderm-mesoderm lineage in embryoid bodies in?vitro. In Fgf4- and heparin-supplemented culture conditions, Tet1-depleted ESCs activate the trophoblast stem cell lineage determinant Elf5 and can colonize the placenta in midgestation embryo chimeras. Consistent with these findings, Tet1-depleted ESCs?form aggressive hemorrhagic teratomas with increased endoderm, reduced neuroectoderm, and ectopic appearance of trophoblastic giant cells. Thus, 5hmC is an epigenetic modification associated with the pluripotent state, and Tet1 functions to regulate the lineage differentiation potential of ESCs. 相似文献
99.
Yanming Wu Xiao Chen Qian Zhou Qizhi He Jiuhong Kang Jing Zheng Kai Wang Tao Duan 《PloS one》2014,9(1)
Vascular remodeling in the placenta is essential for normal fetal development. The previous studies have demonstrated that in utero exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, an environmental toxicant) induces the intrauterine fetal death in many species via the activation of aryl hydrocarbon receptor (AhR). In the current study, we compared the effects of 2-(1′H-indole-3′-carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE) and TCDD on the vascular remodeling of rat placentas. Pregnant rats on gestational day (GD) 15 were randomly assigned into 5 groups, and were exposed to a single dose of 1.6 and 8.0 mg/kg body weight (bw) ITE, 1.6 and 8.0 µg/kg bw TCDD, or an equivalent volume of the vehicle, respectively. The dams were sacrificed on GD20 and the placental tissues were gathered. The intrauterine fetal death was observed only in 8.0 µg/kg bw TCDD-exposed group and no significant difference was seen in either the placental weight or the fetal weight among all these groups. The immunohistochemical and histological analyses revealed that as compared with the vehicle-control, TCDD, but not ITE, suppressed the placental vascular remodeling, including reduced the ratio of the placental labyrinth zone to the basal zone thickness (at least 0.71 fold of control), inhibited the maternal sinusoids dilation and thickened the trophoblastic septa. However, no marked difference was observed in the density of fetal capillaries in the labyrinth zone among these groups, although significant differences were detected in the expression of angiogenic growth factors between ITE and TCDD-exposed groups, especially Angiopoietin-2 (Ang-2), Endoglin, Interferon-γ (IFN-γ) and placenta growth factor (PIGF). These results suggest ITE and TCDD differentially regulate the vascular remodeling of rat placentas, as well as the expression of angiogenic factors and their receptors, which in turn may alter the blood flow in the late gestation and partially resulted in intrauterine fetal death. 相似文献
100.
采用紫外光谱法和荧光光谱法研究了茶碱与胃蛋白酶的结合作用。观测到茶碱使胃蛋白酶的紫外吸收峰增强,特征荧光峰淬灭。Stern-Volmer淬灭曲线显示,茶碱对胃蛋白酶的荧光淬灭很可能是一个单一的静态淬灭过程。 相似文献