全文获取类型
收费全文 | 620篇 |
免费 | 35篇 |
国内免费 | 2篇 |
出版年
2023年 | 5篇 |
2022年 | 19篇 |
2021年 | 34篇 |
2020年 | 19篇 |
2019年 | 25篇 |
2018年 | 27篇 |
2017年 | 13篇 |
2016年 | 24篇 |
2015年 | 33篇 |
2014年 | 33篇 |
2013年 | 56篇 |
2012年 | 60篇 |
2011年 | 47篇 |
2010年 | 24篇 |
2009年 | 29篇 |
2008年 | 40篇 |
2007年 | 35篇 |
2006年 | 23篇 |
2005年 | 29篇 |
2004年 | 28篇 |
2003年 | 17篇 |
2002年 | 18篇 |
2001年 | 2篇 |
2000年 | 2篇 |
1999年 | 3篇 |
1998年 | 2篇 |
1997年 | 1篇 |
1995年 | 1篇 |
1993年 | 2篇 |
1990年 | 1篇 |
1981年 | 1篇 |
1980年 | 2篇 |
1979年 | 2篇 |
排序方式: 共有657条查询结果,搜索用时 421 毫秒
651.
David C. Aldridge Isobel S. Ollard Yulia V. Bespalaya Ivan N. Bolotov Karel Douda Juergen Geist Wendell R. Haag Michael W. Klunzinger Manuel Lopes-Lima Musa C. Mlambo Nicoletta Riccardi Ronaldo Sousa David L. Strayer Santiago H. Torres Caryn C. Vaughn Tadeusz Zając Alexandra Zieritz 《Global Change Biology》2023,29(3):575-589
We identified 14 emerging and poorly understood threats and opportunities for addressing the global conservation of freshwater mussels over the next decade. A panel of 17 researchers and stakeholders from six continents submitted a total of 56 topics that were ranked and prioritized using a consensus-building Delphi technique. Our 14 priority topics fell into five broad themes (autecology, population dynamics, global stressors, global diversity, and ecosystem services) and included understanding diets throughout mussel life history; identifying the drivers of population declines; defining metrics for quantifying mussel health; assessing the role of predators, parasites, and disease; informed guidance on the risks and opportunities for captive breeding and translocations; the loss of mussel–fish co-evolutionary relationships; assessing the effects of increasing surface water changes; understanding the effects of sand and aggregate mining; understanding the effects of drug pollution and other emerging contaminants such as nanomaterials; appreciating the threats and opportunities arising from river restoration; conserving understudied hotspots by building local capacity through the principles of decolonization; identifying appropriate taxonomic units for conservation; improved quantification of the ecosystem services provided by mussels; and understanding how many mussels are enough to provide these services. Solutions for addressing the topics ranged from ecological studies to technological advances and socio-political engagement. Prioritization of our topics can help to drive a proactive approach to the conservation of this declining group which provides a multitude of important ecosystem services. 相似文献
652.
Kvichansky Alexey A. Tret’yakova Liya V. Volobueva Maria N. Manolova Anna O. Stepanichev Mikhail Yu. Onufriev Mikhail V. Moiseeva Yulia V. Lazareva Natalia A. Bolshakov Alexey P. Gulyaeva Natalia V. 《Biochemistry. Biokhimii?a》2021,86(6):693-703
Biochemistry (Moscow) - Differential effect of the neonatal proinflammatory stress (NPS) on the development of neuroinflammation in the hippocampus and induction of the depressive-like behavior in... 相似文献
653.
Abdelazeez Walla Mohamed Abdelmaksood Anatolievna Kostyukova Yulia Zavdetovna Khusnetdinova Landysh Damirovna Alekbaeva Gulnaz Abou El-Dis Gamil Rayan Arnoldovna Timofeeva Olga 《In vitro cellular & developmental biology. Plant》2022,58(4):593-605
In Vitro Cellular & Developmental Biology - Plant - For the first time, suspension cultures produced tropane alkaloids and were obtained from root explants of Hyoscyamus muticus L. It was shown... 相似文献
654.
Maria S Fadeeva Cinthia Nú?ez Yulia V Bertsova Guadalupe Espín Alexander V Bogachev 《FEMS microbiology letters》2008,279(1):116-123
The catalytic properties of sodium-translocating NADH:quinone oxidoreductases (Na+-NQRs) from the marine bacterium Vibrio harveyi, the enterobacterium Klebsiella pneumoniae, and the soil microorganism Azotobacter vinelandii have been comparatively analyzed. It is shown that these enzymes drastically differ in their affinity to sodium ions. The enzymes also possess different sensitivity to inhibitors. Na+-NQR from A. vinelandii is not sensitive to low 2-n-heptyl-4-hydroxyquinoline N-oxide (HQNO) concentrations, while Na+-NQR from K. pneumoniae is fully resistant to either Ag+ or N-ethylmaleimide. All the Na+-NQR-type enzymes are sensitive to diphenyliodonium, which is shown to modify the noncovalently bound FAD of the enzyme. 相似文献
655.
Kinetochore individualization in meiosis I is required for centromeric cohesin removal in meiosis II
Yulia Gryaznova Leonor Keating Sandra A Touati Damien Cladire Warif El Yakoubi Eulalie Buffin Katja Wassmann 《The EMBO journal》2021,40(7)
Partitioning of the genome in meiosis occurs through two highly specialized cell divisions, named meiosis I and meiosis II. Step‐wise cohesin removal is required for chromosome segregation in meiosis I, and sister chromatid segregation in meiosis II. In meiosis I, mono‐oriented sister kinetochores appear as fused together when examined by high‐resolution confocal microscopy, whereas they are clearly separated in meiosis II, when attachments are bipolar. It has been proposed that bipolar tension applied by the spindle is responsible for the physical separation of sister kinetochores, removal of cohesin protection, and chromatid separation in meiosis II. We show here that this is not the case, and initial separation of sister kinetochores occurs already in anaphase I independently of bipolar spindle forces applied on sister kinetochores, in mouse oocytes. This kinetochore individualization depends on separase cleavage activity. Crucially, without kinetochore individualization in meiosis I, bivalents when present in meiosis II oocytes separate into chromosomes and not sister chromatids. This shows that whether centromeric cohesin is removed or not is determined by the kinetochore structure prior to meiosis II. 相似文献
656.
657.
Alla O. Badyakina Natalia V. Vasil’eva Yulia A. Koryakina Elena V. Anisimova Natalia E. Suzina Marina A. Nesmeyanova 《Central European Journal of Biology》2007,2(3):385-404
Secretion of periplasmic alkaline phosphatase (PhoA) encoded by the gene constituent of plasmids and the peculiar properties
of cell envelope biogenesis in Escherichia coli strains with controlled synthesis of individual membrane phospholipids have been studied. Alkaline phosphatase secretion
across the cytoplasmic membrane declines, while secretion into the culture medium intensifies under changed metabolism. The
composition of anionic membrane phospholipids changes due to inactivation of the pgsA gene or regulation of its expression by environmental factor, as well as in the absence of the pssA gene which is responsible for the synthesis of the precursor for zwitter-ionic phospholipid — phosphatidylethanolamine. This
correlates with intensified secretion of exopolysaccharides and lower content of lipopolysaccharide and lipoprotein which
are responsible for barrier properties of the outer membrane. The results suggest a possible coupling of protein secretion
with biogenesis of cell envelope components at a level of phospholipid metabolism. 相似文献