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531.
Sachiyo Nagi Evans A. Chadeka Toshihiko Sunahara Faith Mutungi Yombo K. Dan Justin Satoshi Kaneko Yoshio Ichinose Sohkichi Matsumoto Sammy M. Njenga Masahiro Hashizume Masaaki Shimada Shinjiro Hamano 《PLoS neglected tropical diseases》2014,8(7)
Background
An increasing risk of Schistosoma mansoni infection has been observed around Lake Victoria, western Kenya since the 1970s. Understanding local transmission dynamics of schistosomiasis is crucial in curtailing increased risk of infection.Methodology/Principal Findings
We carried out a cross sectional study on a population of 310 children from eight primary schools. Overall, a total of 238 (76.8%) children were infected with S. mansoni, while seven (2.3%) had S. haematobium. The prevalence of hookworm, Trichuris trichiura and Ascaris lumbricoides were 6.1%, 5.2% and 2.3%, respectively. Plasmodium falciparum was the only malaria parasite detected (12.0%). High local population density within a 1 km radius around houses was identified as a major independent risk factor of S. mansoni infection. A spatial cluster of high infection risk was detected around the Mbita causeway following adjustment for population density and other potential risk factors.Conclusions/Significance
Population density was shown to be a major factor fuelling schistosome infection while individual socio-economic factors appeared not to affect the infection risk. The high-risk cluster around the Mbita causeway may be explained by the construction of an artificial pathway that may cause increased numbers of S. mansoni host snails through obstruction of the waterway. This construction may have, therefore, a significant negative impact on the health of the local population, especially school-aged children who frequently come in contact with lake water. 相似文献532.
Kazumi Funane Hitomi Ichinose Motomi Araki Ryuichiro Suzuki Keitarou Kimura Zui Fujimoto Mikihiko Kobayashi Atsuo Kimura 《Applied microbiology and biotechnology》2014,98(9):3947-3954
Bacillus circulans T-3040 produces cycloisomaltooligosaccharide glucanotransferase (CITase) and cycloisomaltooligosaccharides (cyclodextrans, CIs) when it is grown in media containing dextran as the carbon source. To investigate the effects of carbon sources on CITase activity, B. circulans T-3040 was cultured with glucose; sucrose; a mixture of isomaltose, isomaltotriose, and panose (IMOs); a mixture of maltohexaose and maltoheptaose (G67); dextrin (average degree of polymerization?=?36); dextran 40; and soluble starch. In addition to dextran 40, CIs were produced when the T-3040 strain was grown in media containing soluble starch as the sole carbon source. CITase production was induced by dextran 40, IMOs, and soluble starch but not by G67 or dextrin, which suggests that α-1,6 glucosidic linkages are required for CITase induction. Although CITase was induced by IMOs, no CIs were produced in the culture. CI-producing activity in the presence of soluble starch as the substrate (SS-CITase activity) was observed only in cultures containing dextran 40 or soluble starch. The production of CITase was significantly unaffected by glucose addition, but SS-CITase activity almost completely disappeared after glucose addition. A 135-kDa protein was found to contribute to CI formation from starch in the presence of CITase. This protein had a disproportionation activity with maltooligosaccharides, and its induction and inhibition system may be different from those of CITase. 相似文献
533.
The moss Physcomitrella patens has two RNA editing sites in the chloroplasts. Here we identified a novel DYW-subclass pentatricopeptide repeat (PPR) protein, PpPPR_45, as a chloroplast RNA editing factor in P. patens. Knockdown of the PpPPR_45 gene reduced the extent of RNA editing at the chloroplast rps14-C2 site, whereas over-expression of PpPPR_45 increased the levels of RNA editing at both the rps14-C2 site and its neighboring C site. This indicates that the expression level of PpPPR_45 affects the extent of RNA editing at the two neighboring sites. 相似文献
534.
Feng Xu Yusuke Sudo Sho Sanechika Junpei Yamashita Sho Shimaguchi Shun-ichiro Honda Chiho Sumi-Ichinose Masayo Mori-Kojima Rieko Nakata Tadaomi Furuta Minoru Sakurai Masahiro Sugimoto Tomoyoshi Soga Kazunao Kondo Hiroshi Ichinose 《FEBS letters》2014
Quinonoid dihydropteridine reductase (QDPR) catalyzes the regeneration of tetrahydrobiopterin (BH4), a cofactor for monoamine synthesis, phenylalanine hydroxylation and nitric oxide production. Here, we produced and analyzed a transgenic Qdpr−/− mouse model. Unexpectedly, the BH4 contents in the Qdpr−/− mice were not decreased and even increased in some tissues, whereas those of the oxidized form dihydrobiopterin (BH2) were significantly increased. We demonstrated that unlike the wild-type mice, dihydrofolate reductase regenerated BH4 from BH2 in the mutants. Furthermore, we revealed wide alterations in folate-associated metabolism in the Qdpr−/− mice, which suggests an interconnection between folate and biopterin metabolism in the transgenic mouse model. 相似文献
535.
Tomoko Maehara Zui Fujimoto Hitomi Ichinose Mari Michikawa Koichi Harazono Satoshi Kaneko 《The Journal of biological chemistry》2014,289(11):7962-7972
α-l-Arabinofuranosidase, which belongs to the glycoside hydrolase family 62 (GH62), hydrolyzes arabinoxylan but not arabinan or arabinogalactan. The crystal structures of several α-l-arabinofuranosidases have been determined, although the structures, catalytic mechanisms, and substrate specificities of GH62 enzymes remain unclear. To evaluate the substrate specificity of a GH62 enzyme, we determined the crystal structure of α-l-arabinofuranosidase, which comprises a carbohydrate-binding module family 13 domain at its N terminus and a catalytic domain at its C terminus, from Streptomyces coelicolor. The catalytic domain was a five-bladed β-propeller consisting of five radially oriented anti-parallel β-sheets. Sugar complex structures with l-arabinose, xylotriose, and xylohexaose revealed five subsites in the catalytic cleft and an l-arabinose-binding pocket at the bottom of the cleft. The entire structure of this GH62 family enzyme was very similar to that of glycoside hydrolase 43 family enzymes, and the catalytically important acidic residues found in family 43 enzymes were conserved in GH62. Mutagenesis studies revealed that Asp202 and Glu361 were catalytic residues, and Trp270, Tyr461, and Asn462 were involved in the substrate-binding site for discriminating the substrate structures. In particular, hydrogen bonding between Asn462 and xylose at the nonreducing end subsite +2 was important for the higher activity of substituted arabinofuranosyl residues than that for terminal arabinofuranoses. 相似文献
536.
The present study reports the successful and effective degradation of the persistent herbicide dymron catalyzed by the oxidative enzyme laccase in the presence of a reaction mediator (a laccase/mediator system). Using 2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as the mediator, over 90% of dymron was degraded within 24 h, while the half-life of dymron is 50 days in soil. The results suggested that oxidation of dymron resulted in the production of decomposed compounds with a single aromatic ring. We also found that edible surfactants and a dishwashing detergent were useful to solubilize dymron in an aqueous solution and did not inhibit the oxidative degradation. Degradation proceeded at acidic pH and in a broad range of temperatures (303-353 K). The use of natural mediators also allowed the oxidative degradation of dymron to some extent. In conclusion, we propose the possible use of a laccase/mediator system for the treatment of soils and drainwater contaminated with herbicides. 相似文献
537.
538.
Sachi Kashihara Takafumi Nishimura Yoshiteru Noutoshi Mikihiro Yamamoto Kazuhiro Toyoda Yuki Ichinose Hidenori Matsui 《Molecular Plant Pathology》2022,23(6):885
Pseudomonas amygdali pv. tabaci (formerly Pseudomonas syringae pv. tabaci; Pta) is a gram‐negative bacterium that causes bacterial wildfire disease in Nicotiana tabacum. The pathogen establishes infections by using a type III secretion system to inject type III effector proteins (T3Es) into cells, thereby interfering with the host__s immune system. To counteract the effectors, plants have evolved disease‐resistance genes and mechanisms to induce strong resistance on effector recognition. By screening a series of Pta T3E‐deficient mutants, we have identified HopAZ1 as the T3E that induces disease resistance in N. tabacum ‘N509’. Inoculation with the Pta ∆hopAZ1 mutant did not induce resistance to Pta in N509. We also found that the Pta ∆hopAZ1 mutant did not induce a hypersensitive response and promoted severe disease symptoms in N509. Furthermore, a C‐terminal truncated HopAZ1 abolished HopAZ1‐dependent cell death in N509. These results indicate that HopAZ1 is the avirulence factor that induces resistance to Pta by N509. 相似文献
539.
N Hara M Ichinose M Sawada T Maeno 《Comparative biochemistry and physiology. A, Comparative physiology》1990,97(3):417-421
1. Using the perforated patch recording, the effects of ATP on membrane current were investigated in mouse peritoneal macrophages. 2. Extracellularly applied ATP induced a biphasic current consisting of a initial inward current [Ii(ATP)] followed by an outward current [Io(ATP)]. These currents were associated with a marked increase in conductance at their peaks. 3. Ii(ATP) reversed close to 0 mV and was attenuated by removal of external Na+. 4. Io(ATP) reversed near -80 mV and was increased by decreasing the external concentration of K+. 5. Io(ATP) was completely abolished by removal of external Ca2+, treatment with an intracellular Ca2+ chelator, the acetoxymethyl ester of 1,2-bis (2-aminophenoxy) ethane-N,N,N',N'-tetra acetic acid (BAPTA-AM) and bath applied quinidine but not tetraethylammonium (TEA) or apamin. 6. These results suggest that Ii(ATP) and Io(ATP) are due to an activation of nonspecific cationic and Ca2(+)-dependent K+ conductances, respectively, and raise the possibility that the putative ATP receptor may be important in regulating macrophage functions, motility, phagocytosis and cytokines secretion. 相似文献
540.
Since workers of the ant Aphaenogaster senilis can lay male eggs, reproductive conflict may occur between these workers. We examined the occurrence of worker conflicts
in groups of workers either with or without the queen. Intranidal aggression was observed in each nest for 10 min each day,
and the immatures produced were counted once a week for two months. Pairs of workers involved in aggression were taken regularly
from each nest and used for chemical, morphological and anatomical analyses. The attacker and the attacked workers differed
in their cuticular hydrocarbon profiles. The attacker and the attacked ants were at the same middle-aged fertile stage. The
attacker ant was significantly larger and more fertile than the attacked ant, and more mature physiologically (poison gland
was darker). There was apparently no stable hierarchy between laying workers. In the first weeks under queenless conditions,
most eggs and larvae were destroyed, but they were later reared to obtain males. The intranidal worker aggression in this
highly evolved ant is discussed in relation to dominance and worker policing.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献