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501.
502.
Ken-ichiro Inoue Hirohisa Takano Rie Yanagisawa Miho Sakurai Takamichi Ichinose Kaori Sadakane Toshikazu Yoshikawa 《Respiratory research》2005,6(1):106
Background
Particulate matter (PM) can exacerbate allergic airway diseases. Although health effects of PM with a diameter of less than 100 nm have been focused, few studies have elucidated the correlation between the sizes of particles and aggravation of allergic diseases. We investigated the effects of nano particles with a diameter of 14 nm or 56 nm on antigen-related airway inflammation.Methods
ICR mice were divided into six experimental groups. Vehicle, two sizes of carbon nano particles, ovalbumin (OVA), and OVA + nano particles were administered intratracheally. Cellular profile of bronchoalveolar lavage (BAL) fluid, lung histology, expression of cytokines, chemokines, and 8-hydroxy-2''-deoxyguanosine (8-OHdG), and immunoglobulin production were studied.Results
Nano particles with a diameter of 14 nm or 56 nm aggravated antigen-related airway inflammation characterized by infiltration of eosinophils, neutrophils, and mononuclear cells, and by an increase in the number of goblet cells in the bronchial epithelium. Nano particles with antigen increased protein levels of interleukin (IL)-5, IL-6, and IL-13, eotaxin, macrophage chemoattractant protein (MCP)-1, and regulated on activation and normal T cells expressed and secreted (RANTES) in the lung as compared with antigen alone. The formation of 8-OHdG, a proper marker of oxidative stress, was moderately induced by nano particles or antigen alone, and was markedly enhanced by antigen plus nano particles as compared with nano particles or antigen alone. The aggravation was more prominent with 14 nm of nano particles than with 56 nm of particles in overall trend. Particles with a diameter of 14 nm exhibited adjuvant activity for total IgE and antigen-specific IgG1 and IgE.Conclusion
Nano particles can aggravate antigen-related airway inflammation and immunoglobulin production, which is more prominent with smaller particles. The enhancement may be mediated, at least partly, by the increased local expression of IL-5 and eotaxin, and also by the modulated expression of IL-13, RANTES, MCP-1, and IL-6. 相似文献503.
Inoue K Takano H Yanagisawa R Sakurai M Ichinose T Sadakane K Hiyoshi K Sato M Shimada A Inoue M Yoshikawa T 《Experimental biology and medicine (Maywood, N.J.)》2005,230(1):75-81
Metallothionein (MT) is a protein that can be induced by inflammatory mediators and participates in cytoprotection. However, its role in antigen-related inflammation remains to be established. We determined whether intrinsic MT protects against antigen-related airway inflammation induced by ovalbumin (OVA) in MT-I/II null (MT [-/-]) mice and in corresponding wild-type (WT) mice. MT (-/-) mice and WT mice were intratracheally challenged with OVA (1 mug per body) biweekly four times. Twenty-four hours after the last OVA challenge, significant increases were shown in the numbers of total cells, eosinophils, and neutrophils in bronchoalveolar lavage fluid from MT (-/-) mice than in those from WT mice. The protein level of interleukin-1beta (IL-1beta) was significantly greater in MT (-/-) mice than in WT mice after OVA challenge. Immunohistochemical analysis showed that the formations of 8-oxy-deoxyguanosine and nitrotyrosine in the lung were more intense in MT (-/-) mice than in WT mice after OVA challenge. These results indicate that endogenous MT is a protective molecule against antigen-related airway inflammation induced by OVA, at least partly, via the suppression of enhanced lung expression of IL-1beta and via the antioxidative properties. Our findings suggest that MT may be a therapeutic target for the treatment of antigen-related airway inflammatory diseases such as bronchial asthma. 相似文献
504.
Mutation Detection in DNA Oligonucleotides Based on a Guanine Quenching Method Coupled with Enzymatic Digestion of Single-Stranded DNA 总被引:1,自引:0,他引:1
Maruyama T Shinohara T Ichinose H Kitaoka M Okamura N Kamiya N Goto M 《Biotechnology letters》2005,27(18):1349-1354
Fluorescence quenching by guanine allows DNA hybridization to be monitored and any point mutations in oligonucleotides to
be detected. However, fluorescence quenching is often affected by untargeted guanine located in a protruding end (single-strand
DNA) of the probe-target DNA duplex resulting in an unsatisfactory sensitivity. In the present study, we used enzymatic digestion
of the protruding end of a probe-target DNA duplex to avoid interference by untargeted guanine on fluorescence quenching for
detection of a nucleobase mutation. Enzymatic digestion of the protruding end of the DNA duplex fully prevented interference
by untargeted guanine, and produced a marked difference in the quenching ratios (36% for wild-type, and 0% for mutant). 相似文献
505.
Akgul G Erturk A Turkoz M Turan T Ichinose A Nagatake T Ahmed K 《Microbiology and immunology》2005,49(10):931-935
The goal of this study was to determine the role of lipooligosaccharide in the attachment of Moraxella catarrhalis to human pharyngeal epithelial cells. Strain 2951 and its P(k) mutant strain 2951 galE were used in this study. This study suggests that the P(k) epitope of LOS is not an adhesin for M. catarrhalis, but plays a crucial role by its surface charge in the initial stage of attachment. 相似文献
506.
In vitro cartilage formation of composites of synovium-derived mesenchymal stem cells with collagen gel 总被引:2,自引:0,他引:2
Yokoyama A Sekiya I Miyazaki K Ichinose S Hata Y Muneta T 《Cell and tissue research》2005,322(2):289-298
Graft implantation is one of the more popular procedures for repairing cartilage defects; however, sacrifices of the donor
site have been an issue. Mesenchymal stem cells (MSCs) are a fascinating source for regenerative medicine because they can
be harvested in a less invasive manner and are easily isolated and expanded, with multipotentiality including chondrogenesis.
MSCs can be isolated from various adult mesenchymal tissues including synovium. Here, we attempted to form cartilage from
the composites of synovium-derived MSCs with collagen gel in vitro. After 21 days of culture, the composites had increased
their cartilage matrix, as demonstrated by toluidine blue staining and immunohistochemistry for type II collagen. The composites
consisting of 5×107 and 108 cells/ml in gel were richer in proteoglycans than those consisting of lower cell densities. After 1 day, MSCs/gel composites
contracted and the diameter decreased by 30%; however, they were stable thereafter. Round cells with short processes producing
collagen fibrils showing a similar morphology to that of chondrocytes were seen in the composites by transmission electron
microscopy. During composite culture, chondroitin sulfate and mRNA expression for cartilage-related genes increased, demonstrating
cartilage maturation. Using an optimized method, we obtained cartilage discs with a diameter of 7 mm and a thickness of 500 μm.
Our procedure should thus make it possible to produce a large cartilage matrix in vitro. The tissue engineering of autologous
cartilage from the composites of synovium-derived MSCs with collagen gel in vitro for transplantation may be a future alternative
to graft implantation for patients with cartilage defects.
This study is supported in part by grants from the Japanese Society for the Promotion of Science (16591478), the Japanese
Orthopaedics and Traumatology Foundation, and the Nakatomi Foundation to I.S., and the Japanese Society for the Promotion
of Science (16591477), the Japanese Sports Medicine Foundation, the Japanese Latest Osteoarthritis Society, and the Center
of Excellence Program for Frontier Research on Molecular Destruction and Reconstruction of Tooth and Bone in Tokyo Medical
and Dental University to T.M. 相似文献
507.
Ohtsuki M Shiraishi H Kato T Kuroda R Tazawa M Sumi-Ichinose C Tada S Udagawa Y Itoh M Hishida H Ichinose H Nagatsu T Hagino Y Nomura T 《Life sciences》2002,70(18):2187-2198
We studied the effects of cAMP on cytokine (interferon-gamma plus tumor necrosis factor-alpha)-induced stimulation of tetrahydrobiopterin (BH4) synthesis in human umbilical vein endothelial cells (HUVEC). The cytokine mixture caused a marked increase in the biosynthesis and release of BH4 by HUVEC. Dibutyryl-cAMP produced a dose-dependent inhibition of this cytokine-induced stimulation of synthesis and release of BH4 by these cells. 8-Bromo-cAMP also caused a significant inhibition, although the effects were less marked than those of dibutyryl-cAMP. Both forskolin and the stable analog of prostacyclin, iloprost, caused cAMP accumulation and a concomitant diminution of the cytokine-induced BH4 synthesis in HUVEC. Dibutyryl-cAMP and iloprost also significantly inhibited the cytokine-induced stimulation of GTP cyclohydrolase I (GCHI) activity and mRNA production. We concluded that the suppression by the cAMP messenger system of cytokine-induced stimulation of synthesis and release of BH4 by HUVEC can be attributed to the inhibition of the activity of GCHI, the rate-limiting enzyme in BH4 biosynthetic pathway, in HUVEC. The data also suggest that the cAMP-mediated reduction in the GCHI mRNA level may at least partially explain the decline in GCHI activity. It is reasoned that under inflammatory conditions, cAMP-elevating agents such as prostacyclin exert regulatory effects on circulation by inhibiting cytokine-induced synthesis and release of BH4 by HUVEC. 相似文献
508.
Ito M Okui H Nakagawa H Mio S Kinoshita A Obayashi T Miura T Nagai J Yokoi S Ichinose R Tanaka K Kodama S Iwasaki T Miyake T Takashio M Iwabuchi J 《Bioscience, biotechnology, and biochemistry》2002,66(11):2406-2414
A new series of N-oxydihydropyrrole derivatives was synthesized and evaluated for insecticidal activity against Nilaparvata lugens and Myzus persicae. Various substituents were introduced to the 1-position of the dihydropyrrole ring, and the derivatives obtained exhibited systemic and/or contact insecticidal activity. The structure-activity relationship revealed that small alkyoxy and alkoxyalkoxy groups were more favorable than alkylcarbonyloxy, alkoxycarbonyloxy, or sulfonyloxy groups as substituents at the 1-position. 相似文献
509.
510.
Magari H Shimizu Y Inada K Enomoto S Tomeki T Yanaoka K Tamai H Arii K Nakata H Oka M Utsunomiya H Tsutsumi Y Tsukamoto T Tatematsu M Ichinose M 《Biochemical and biophysical research communications》2005,334(2):606-612
The effect of the selective COX-2 inhibitor, etodolac, on Helicobacter pylori (Hp)-associated stomach carcinogenesis was investigated in Mongolian gerbils (MGs). Hp-infected MGs were fed for 23 weeks with drinking water containing 10 ppm N-methyl-N-nitrosourea. They were then switched to distilled water and placed on a diet containing 5-30 mg/kg/day etodolac for 30 weeks. We found that etodolac dose-dependently inhibited the development of gastric cancer, and no cancer was detected at a dose of 30 mg/kg/day. Etodolac did not affect the extent of inflammatory cell infiltration or oxidative DNA damage, but it significantly inhibited mucosal cell proliferation and dose-dependently repressed the development of intestinal metaplasia in the stomachs of Hp-infected MGs. These results suggest that COX-2 is a key molecule in inflammation-mediated stomach carcinogenesis and that chemoprevention of stomach cancer should be possible by controlling COX-2 expression or activity. 相似文献