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排序方式: 共有231条查询结果,搜索用时 15 毫秒
101.
102.
Jun-ichi Kato Yukinobu Nishimura Hideho Suzuki 《Molecular & general genetics : MGG》1989,217(1):178-181
Summary A thermosensitive (ts) parA mutant, MFT110, of Escherichia coli carried at least two ts mutations. The major ts defect, resulting from a mutation mapped originally at 95 min and complemented by pLC8-47, was most probably due to psd. A plasmid carrying the 1.6 kb BamHI-PvuII fragment recloned from pLC8-47 complemented the major ts mutation in MFT110 and psd(ts) in two mutants, but did not correct the Par phenotype of MFT110. The second ts mutation was salt-repairable and mapped at 83 min close to recF and tnaA. This mutation was linked with the Par phenotype as shown unambiguously by 4,6-diamidino-2-phenylindole stained nucleoids in parA mutant cells with the W3110 genetic background. Both salt-repairable ts and Par traits were corrected concomitantly by a plasmid carrying the chromosomal region solely for the gyrB gene. This strongly suggests that parA is an allele of gyrB. 相似文献
103.
Summary The activities of chymotrypsin-like esteroproteases in the mouse submandibular gland were additively induced by 5-dihydrotestosterone (DHT) and triiodothyronine (T3). Zymograms showed that there are many isozymes whose activities are regulated by DHT and/or T3. Some isozymes seemed to be hormone-independent. Histochemical studies revealed that all these isozymes are localized in the granular convoluted tubules. 相似文献
104.
105.
Aortic glycosaminoglycans in atheroma and alloxan diabetes 总被引:2,自引:0,他引:2
106.
The age-related development of GABABreceptors and their coupling to adenylate cyclase were studied in the brains of spontaneously hypertensive (SHR) and normotensive Wistar-Kyoto (WKY) rats. Compared with WKY rats, the specific [3H]GABA binding to GABABreceptors showed a significant decrease not only in the posterior hypothalamus, midbrain, hippocampus and striatum of eleven-week-old SHR, which maintain a hypertensive state, but also in the posterior hypothalamus of four-week-old normotensive SHR. Similarly, the GABABreceptor agonists (baclofen and DN-2327)-induced suppression of adenylate cyclase activity showed a decrease in the posterior hypothalamus of four-week-old SHR as well as in the posterior hypothalamus and striatum of eleven-week-old SHR. These results suggest that the functions of the GABABreceptor in the brain of SHR may be decreased independently from the occurrence of blood pressure elevation and that such changes may even be involved in the pathogenesis of SHR. 相似文献
107.
Miki Miyagiwa Takafumi Ichida Takayoshi Tokiwa Jiro Sato Hiroshi Sasaki 《In vitro cellular & developmental biology. Plant》1989,25(6):503-510
Summary A human cholangiocellular carcinoma cell line, HuCC-T1, was established in vitro from the malignant cells of ascites of a
56-yr-old patient. Histologic findings of the primary liver tumor revealed a moderately differentiated adenocarcinoma. Tumor
cells from the ascites have been cultured with RPMI 1640 medium containing 0.2% lactalbumin hydrolysate and the cultured cells
grew as monolayers with a population doubling time of 74 h during exponential growth at Passage 25. They had an epithelial-like
morphology and were positive for mucine staining. Ultrastructural studies revealed the presence of microvilli on the cell
surface and poorly developed organelles in the cytoplasm. The HuCC-T1 cell was tumorigenic in nude mice. The number of chromosomes
in HuCC-T1 ranged from 61 to 80. These human cholangiocellular carcinoma cells in serum-free medium secreted several tumor
markers, including carbohydrate antigen 19/9, carbohydrate antigen 125, carcinoembryonic antigen, and tissue polypeptide antigen.
The carbohydrate antigen 19/9 secretion level of HuCC-T1 cells cultured in PRMI 1640 medium with 1% fetal bovine serum was
sixfold higher than that with 0.2% lactalbumin hydrolysate. These findings suggest that HuCC-T1 will provide useful information
to clarify the mechanism of tumor marker secretion and tumor cell growth in the human cholangiocellular carcinoma. 相似文献
108.
Kazuhiro Hirakura Makoto Morita Kaoru Nakajima Yukinobu Ikeya Hiroshi Mitsuhashi 《Phytochemistry》1991,30(12):4053-4055
Three new acetylated polyacetylenes named ginsenoynes F, G and H were isolated from the hexane extract of the roots of Panax ginseng. The structures were determinated by spectral and chemical methods. 相似文献
109.
The structure and absolute configuration of calliterpenone has been established as 3-oxo-13β-kaurane-16α,17-diol. This conclusion confirms that proposed by Ahmad and Zaman, and the formula suggested previously by Chatterjee et al. is revised. 相似文献
110.
Masayuki Sakiyama Hirotaka Matsuo Seiko Shimizu Toshinori Chiba Akiyoshi Nakayama Yuzo Takada Takahiro Nakamura Tappei Takada Emi Morita Mariko Naito Kenji Wakai Hiroki Inoue Seishiro Tatsukawa Junki Sato Kazumi Shimono Toshiaki Makino Takahiro Satoh Hiroshi Suzuki Yoshikatsu Kanai Nobuyuki Hamajima Yutaka Sakurai Kimiyoshi Ichida Toru Shimizu Nariyoshi Shinomiya 《Human cell》2014,27(1):1-4
Gout is a common disease resulting from hyperuricemia which causes acute arthritis. Recently, genome-wide association studies revealed an association between serum uric acid levels and a common variant of leucine-rich repeat-containing 16A (LRRC16A) gene. However, it remains to be clarified whether LRRC16A contributes to the susceptibility to gout. In this study, we investigated the relationship between rs742132 in LRRC16A and gout. A total of 545 Japanese male gout cases and 1,115 male individuals as a control group were genotyped. rs742132 A/A genotype significantly increased the risk of gout, conferring an odds ratio of 1.30 (95 % CI 1.05–1.60; p = 0.015). LRRC16A encodes a protein called capping protein ARP2/3 and myosin-I linker (CARMIL), which serves as an inhibitor of the actin capping protein (CP). CP is an essential element of the actin cytoskeleton, which binds to the barbed end of the actin filament and regulates its polymerization. In the apical membrane of proximal tubular cells in the human kidney, the urate-transporting multimolecular complex (urate transportsome) is proposed to consist of several urate transporters and scaffolding proteins, which interact with the actin cytoskeleton. Thus, if there is a CARMIL dysfunction and regulatory disability in actin polymerization, urate transportsome may be unable to operate appropriately. We have shown for the first time that CARMIL/LRRC16A was associated with gout, which could be due to urate transportsome failure. 相似文献