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71.
Balance between site fidelity and habitat preferences in colony site selection by herons and egrets 下载免费PDF全文
Habitat selection in avian species is a hierarchical process driven by different factors acting at multiple scales. Habitat preferences and site fidelity are two main factors affecting how colonial birds choose their breeding locations. Although these two factors affect how colonial species choose their habitats, previous studies have only focused on one factor at a time to explain the distribution of species at regional scales. Here we used 28 yr of colony location data of herons and egrets around Ibaraki prefecture in Japan in order to analyze the relative importance of habitat preferences and colony site fidelity for selecting breeding locations. We used Landsat satellite images together with a ground survey‐based map to create land‐use maps for past years and determine the habitats surrounding the herons and egrets colonies. Combining the estimated colony site fidelity with the habitat data, we used a random forest algorithm to create habitat selection models, which allowed us to analyze the changes in the importance of those factors over the years. We found high levels of colony site fidelity for each year of study, with its relative importance as a predictor for explaining colony distribution increasing drastically in the most recent five years. The increase in collective site fidelity could have been caused by recent changes in the population size of grey herons Ardea cinerea, a key species for colony establishment. We observed a balance between habitat preferences and colony site fidelity: habitat preferences were a more powerful predictor of colony distribution until 2008, when colony site fidelity levels were lower. Considering changes in the relative importance of these factors can lead to a better understanding of the habitat selection process and help to analyze bird species’ responses to environmental changes. 相似文献
72.
Yasuhiro Yamasaki Daisuke Katsuo Seiichiro Nakayasu Cristina Salati JingJing Duan Yanan Zou Yukihiko Matsuyama Kenichi Yamaguchi Tatsuya Oda 《Journal of biochemical and molecular toxicology》2008,22(6):405-415
Our recent studies have demonstrated that the aqueous extract prepared from Alexandrium tamarense, a harmful red tide phytoplankton, showed cytotoxicity on Vero cells. In this study, the toxic substance was purified from the culture supernatant of A. tamarense. Based on the gel‐filtration profile, the molecular mass of a purified toxin was estimated to be about 1,000 kDa. On sodium dodecylsulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) analysis, a main band with molecular mass of 1,000 kDa was detected with periodic acid‐Schiff (PAS) staining, but no protein bands were detected by Coomassie brilliant blue (CBB) protein staining. Sugar composition analysis of the toxin suggested that the toxin contains galactose, fucose, mannose, N‐acetylglucosamine, xylose, and other minor saccharides, whereas no significant levels of amino acids were detected by amino acid analysis. These results suggest that the toxin is a polysaccharide‐based compound. The toxin showed cytotoxic effects on various cell lines in a concentration‐dependent manner. Among the cell lines tested, U937 cells were the most susceptible to the toxin. In U937 cells treated with the toxin, a typical apoptotic nuclear morphological change and DNA fragmentation were observed. This is the first report demonstrating that a polysaccharide‐based toxin isolated from red tide phytoplankton can induce apoptotic cell death. © 2008 Wiley Periodicals, Inc. J Biochem Mol Toxicol 22:405–415, 2008; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/jbt.20253 相似文献
73.
Yukihiko Hayashi Moto Kitayaki Kayoko Takezaki Hidekazu Ikeuchi Shigeru Kunugi 《Biocatalysis and Biotransformation》2003,21(1):25-31
The contribution of three exoglucanases from a commercial Trichoderma viride cellulase to transcellobiosylation, and the tolerance of these enzymes to acetonitrile co-solvent were studied. The enzymatic reactions were performed with p-nitrophenyl-β-d-cellobioside as the starting substrate. Among these enzymes, the least anionic exoglucanase (Exo I) showed the highest transcellobiosylation activity and acetonitrile tolerance. Exo I retained considerable activity even in 30% MeCN/water and produced p-nitrophenyl-β-d-cellotetraoside at about 1.5% conversion from the initial substrate in 30% MeCN/water. The residual activity of Exo I after incubation in MeCN/water mixture was almost identical to that of the crude cellulase and a considerable amount of the transcellobiosylation properties of the crude cellulase seemed to be attributable to this Exo I component. 相似文献
74.
Asexual life history by quadriflagellate swarmers of Ulva spinulosa (Ulvales, Ulvophyceae) 总被引:1,自引:0,他引:1
Masanori Hiraoka Satoshi Shimada Masao Ohno Yukihiko Serisawa 《Phycological Research》2003,51(1):29-34
This is the first report of a Ulva species reproducing asexually solely by quadriflagellate swarmers. Ulva spinulosa Okamura et Segawa specimens were collected from Ukibuchi on the Pacific coast of Kochi Prefecture, southern Japan. Quadriflagellate swarmers were released from these specimens. The swarmers showed negative phototaxis before settlement. Thalli cultured from these swarmers also released quadriflagellate swarmers in culture. Microspectrophotometric studies demonstrated equivalent DNA in nuclei of vegetative cells in thalli of U. spinulosa and in sporo‐phytes of the other Ulva species with sexual life history (U. fasciata Delile). Furthermore, the quadriflagellate swarmers of U. spinulosa had the same DNA value, demonstrating that the quadriflagellate swarmers are produced without meiosis. 相似文献
75.
76.
Shuya Yano Yong Zhang Ming Zhao Yukihiko Hiroshima Shinji Miwa Fuminari Uehara 《Cell cycle (Georgetown, Tex.)》2014,13(24):3958-3963
Quiescent cancer cells are resistant to cytotoxic agents which target only proliferating cancer cells. Time-lapse imaging demonstrated that tumor-targeting Salmonella typhimurium A1-R (A1-R) decoyed cancer cells in monolayer culture and in tumor spheres to cycle from G0/G1 to S/G2/M, as demonstrated by fluorescence ubiquitination-based cell cycle indicator (FUCCI) imaging. A1-R infection of FUCCI-expressing subcutaneous tumors growing in nude mice also decoyed quiescent cancer cells, which were the majority of the cells in the tumors, to cycle from G0/G1 to S/G2/M, thereby making them sensitive to cytotoxic agents. The combination of A1-R and cisplatinum or paclitaxel reduced tumor size compared with A1-R monotherapy or cisplatinum or paclitaxel alone. The results of this study demonstrate that A1-R can decoy quiescent cancer cells to cycle to S/G2/M and sensitize them to cytotoxic chemotherapy. These results suggest a new paradigm of bacterial-decoy chemotherapy of cancer. 相似文献
77.
Atushi Ushimaru Satoshi Kikuchi Ryuji Yonekura Atsushi Maruyama Nao Yanagisawa Maiko Kagami Michiko Nakagawa Suzuki Mahoro Yukihiko Kohmatsu Aya Hatada Shumpei Kitamura Kensuke Nakata 《Nordic Journal of Botany》2004,24(5):593-598
We compared the amount of variation in flower size between autogamous and insect-pollinated species to examine the hypothesis that pollinator-mediated selection stabilizes flower size in plant populations. One would expect the flower size variation to be larger in selfing species that are less affected by pollinator-mediated stabilizing selection than in insect-pollinated species. The results of phylogenetic comparisons between autogamous and insect-pollinated flowers supported the pollinator-mediated stabilizing selection hypothesis, although the non-phylogenetic comparison did not. According to our results, we discuss the factors influencing the flower size variation. 相似文献
78.
Atsushi Sato Nobuo Ida Mari Ishikawa Kazuhiro Tanahashi Haruji Nakamura Yukihiko Sho Terukatsu Arima Tetsunosuke Kunitomo 《Microbiology and immunology》1993,37(4):295-304
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of anti-HCV antibody. We assayed for antibodies against either oligopeptide (S29-1) deduced from the nucleocapsid gene or the product of nonstructural region (NS3) synthesized in a recombinant Escherichia coli (S4). To reduce false-positive results induced by non-specific binding of antibodies with a carrier protein and to increase the sensitivity of an immunoassay, non-fused S4 peptide was prepared by the recombinant DNA technique and site-specific proteolysis (by factor Xa). In 71 non-A, non-B hepatitis patients with chronic liver disease, 70 (98.5%) were positive by S29-1/S4 ELISA as well as by a second-generation test (Abbott II). On the other hand, of 40 serum samples from blood donors, in which anti-N14 (core) and C100-3 antibodies were not detected but hepatitis C virus (HCV) RNA was detectable by polymerase chain reaction (PCR), 24 (60%) were positive by S29-1/S4 ELISA, whereas only 18 (45%) were diagnosed by Abbott II. In addition, based on results in a small group of 92 blood donors, detection of anti-S29-1/S4 antibody correlated well with HCV viremia as confirmed by PCR. These results indicated that the preparation of non-fused protein (S4) by recombinant DNA technique and a combination of S29-1 and S4 as immobilized antigens in an ELISA provide a sensitive and specific diagnosis for HCV infection with good correlation with the presence of viral RNA as confirmed by PCR. 相似文献
79.